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1.16891 / 1.16886 Fractogel® EMD COO- (S), (M) Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and chemical stability. Since the functional ion exchanger groups are bonded via linear polymer chains, the ionic groups are accessible for proteins. Fractogel® EMD COO- is a chromatographic support for the purification of basic and neutral proteins and peptides. Due to the titration behaviour the ion exchange capacity can be used from pH 6 up to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- when the pH of the buffer is about 1 unit below their isoelectric points (pl). The strength of the binding depends on: the buffer system pH value of the buffer which determines the surface charge of the protein the degree of the ionisation of the functional groups of the exchanger the concentration of the counter ions the charge density on the support (protein binding capacity) • • • • • Properties of the tentacle ion exchanger Cat. No. 1.16891, S-Type 1.16886, M-Type Bulk material 100 ml, 500 ml, 5 l (S) 100 ml, 500 ml, 5 l (M) Particle size 20 – 40 µm (S) 40 – 90 µm (M) Type of chromatography Weak cation exchange chromatography Functional group Carboxy ethyl group Protein binding capacity 100 mg lysozyme/ml of gel pK value about 4.5 pH stability range pH 1 up to pH 12 Elution conditions High salt concentrations Pressure limit 8 bar Operating temperature 4 °C to room temperature Storage, preservative 20 % ethanol, 150 mmol/l NaCl Regeneration 1 – 2 M NaCl Sanitization 0.1 – 0.5 M NaOH, sodium lauroyl sarcosinate Linear flow rate Up to 360 cm/h (S-type); up to 400 cm/h (M-type) Merck KGaA · Life Science Products · Darmstadt · Germany [email protected] · www.merck-lsp.de 1/1