Download Course Outline - Pima Community College

Course Content Form
PIMA COMMUNITY COLLEGE
Effective Term: Fall 2009
BIO 206
Credit Hours:
Biotechnology Instrumentation I
4.0
Lecture Periods:
3.0
Lab Periods:
3.0
Description:
Principles and methodologies of recombinant DNA technology. Includes preparation of solutions and growth
Media in a laboratory setting, and genetic analyses.
Course Learning Outcomes:
Upon completion of the course, the student will be able to do the following:
1. Implement the scientific method to answer specific questions.
2. Calibrate and use a variety of common and basic types of lab equipment.
3. Read and follow an instruction manual for lab equipment and lab procedures.
4. Calculate and prepare solutions in various concentrations.
5. Handle, store and dispose of commonly used chemicals and biohazardous materials.
6. Maintain a lab notebook documenting lab procedures, problem solving episodes, data collection, graphing
and analysis, and future recommendations.
7. Assess personal compliance with lab safety procedures.
8. Design an original experiment, assemble the necessary components, collect data, and evaluate the protocol
and outcome.
9. Perform a variety of laboratory techniques routinely used in biotechnology laboratories related to
recombinant DNA technology.
Course Outline:
I.
II.
General Laboratory Skills
A. Operation and Maintenance of Basic Equipment
B. Preparation of Reagents, Media, and Maintenance of Microbial Cultures
C. Good Lab Procedures (GLP)
D. Aseptic Processing
E. Documentation of Lab Work
F. Assay Development and Validation
Genetic Analyses
A. Structure and Function of DNA
B. Transformation and Phage Packaging
C. Genomic DNA and Plasmid DNA Isolation
D. Restriction Digestion, Subcloning, and Ligations
E. Agarose Gel Electrophoresis
F. Southern and Northern Blots
G. RNA Isolation and Reverse Transcription into cDNA
H. DNA Amplification (Polymerase Chain Reaction) and Sequencing