Download BIOCHEMISTRY 4.1 HOMEWORK

BIOCHEMISTRY 4.1 HOMEWORK
NAME____________________________
USING YOUR TEXT, DEFINE THE FOLLOWING TERMS:
Cloning:___________________________________________________________________________________________________
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Vector: __________________________________________________________________________________________________
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Recombinant DNA__________________________________________________________________________________________
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DNA Ligase: _______________________________________________________________________________________________
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Plasmid: __________________________________________________________________________________________________
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Genomics: ________________________________________________________________________________________________
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Complementary DNA: ______________________________________________________________________________________
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Polymerase Chain Reaction: _________________________________________________________________________________
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DNA Fingerprinting_________________________________________________________________________________________
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Southern Blot_____________________________________________________________________________________________
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DNA Microarray____________________________________________________________________________________________
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Transgenic________________________________________________________________________________________________
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1. CLONING. When joining 2 or more DNA fragments, a researcher can adjest the sequence at the junction in a variety of
subtle ways, as seen in the following exercises.
a. Draw the structure of each of a linear DNA fragment produced by an EcoRI restriction digest (include those
sequences remaining from the EcoRI recognition sequence).
b. Draw the structures resulting from the reaction of this end sequence DNA polymerase I and four deoxynucloside
triphosphases (see Fig 8-36 in the book!)
2. Selecting for recombinant plasmids
When cloning a foreign DNA fragment into a plasmid, it is often useful to
insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of pBR322).
The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign
DNA. With a bacteriophage vector, it is not necessary to do this, yet one can easily distinguish vectors that incorporate
large foreign DNA fragments from those that do not. How are these recombinant vectors identified?
3
Suggest how the researcher might use the PCR to detect the presence of the circular form of the deleted DNA in an extract from
the protist.