Bacterial plasmids - Micro-Rao
... Mobilizable plasmids are those plasmids that lack genes to initiate self transfer but do encode the functions needed specifically for transfer of their own DNA. The initiation function is provided by other conjugative plasmid present in the same cell. Suicide plasmids are referred to those plasmids ...
... Mobilizable plasmids are those plasmids that lack genes to initiate self transfer but do encode the functions needed specifically for transfer of their own DNA. The initiation function is provided by other conjugative plasmid present in the same cell. Suicide plasmids are referred to those plasmids ...
PHANTASTICA Regulates Development of the Adaxial Mesophyll in
... is shown in Figure 1 with identical residues shaded. All have a highly conserved MYB DNA binding domain at the N terminus, consisting of two imperfect repeats (55 and 51 residues), both of which are essential for sequence-specific DNA binding based on structural analysis of c-MYB (Jin and Martin, 19 ...
... is shown in Figure 1 with identical residues shaded. All have a highly conserved MYB DNA binding domain at the N terminus, consisting of two imperfect repeats (55 and 51 residues), both of which are essential for sequence-specific DNA binding based on structural analysis of c-MYB (Jin and Martin, 19 ...
Equilibrium Statistics of Channel-confined DNA
... and visualising single DNA molecules have been developed by biologists. Similarly, the development of clean room patterning techniques allows for the manufacturing of well-defined geometries down to the nanometre scale [2, 5, 17]. This has enabled the testing of old polymer theory predictions [18–23 ...
... and visualising single DNA molecules have been developed by biologists. Similarly, the development of clean room patterning techniques allows for the manufacturing of well-defined geometries down to the nanometre scale [2, 5, 17]. This has enabled the testing of old polymer theory predictions [18–23 ...
Heredity & Human Affairs LAB PRACTICAL #1 REVIEW
... The process in which RNA is synthesized from a template strand of DNA is called _______________ and occurs in the ______________ part of cell. ...
... The process in which RNA is synthesized from a template strand of DNA is called _______________ and occurs in the ______________ part of cell. ...
Sequence requirements for function of the
... re-introduced into the Drosophila genome via P elementmediated germline transformation, and these transgenic constructs can amplify with the correct tissue and temporal specificity (deCicco and Spradling, 1984). However, amplification is highly sensitive to chromosomal position effects and only ~1/3 ...
... re-introduced into the Drosophila genome via P elementmediated germline transformation, and these transgenic constructs can amplify with the correct tissue and temporal specificity (deCicco and Spradling, 1984). However, amplification is highly sensitive to chromosomal position effects and only ~1/3 ...
The Johns Hopkins University - American University of Beirut
... • Servers have the records you want • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. ...
... • Servers have the records you want • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. ...
DNA Genetics
... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. ____ 21. Why is it possible for an amino acid to be specified by more than one kind of codon? a. Some codons have ...
... a. each with two new strands. b. one with two new strands and the other with two original strands. c. each with one new strand and one original strand. d. each with two original strands. ____ 21. Why is it possible for an amino acid to be specified by more than one kind of codon? a. Some codons have ...
... The Atlas of Genetics and Cytogenetics in Oncology and Haematology is a peer reviewed on-line journal in open access, devoted to genes, cytogenetics, and clinical entities in cancer, and cancer-prone diseases. It presents structured review articles (“cards”) on genes, leukaemias, solid tumours, canc ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
... lower than the example shown above? What would be the melting temperature of the 18 base pair sequence if it was made up of only G-C pairs? Only A-T pairs? ...
how function during Drosophila metamorphosis
... crooked leg phenotype. This leg defect is associated with inappropriate orientation of leg imaginal discs and the presence of peripodial epithelium that appears to impede leg disc eversion and leg extension, suggesting that how plays a role in interactions between imaginal epithelium, peripodial epi ...
... crooked leg phenotype. This leg defect is associated with inappropriate orientation of leg imaginal discs and the presence of peripodial epithelium that appears to impede leg disc eversion and leg extension, suggesting that how plays a role in interactions between imaginal epithelium, peripodial epi ...
Genetic Polymorphism of Human CYP2E1
... cells transfected with CYP2E1*1 cDNA (Fig. 4C). When 6-hydroxylation of chlorzoxazone was measured in the cell homogenates, a similar decrease was seen in activities as monitored on the protein level. By contrast, cells transfected with CYP2E1*3 cDNA had apoprotein levels and rates of chlorzoxazone ...
... cells transfected with CYP2E1*1 cDNA (Fig. 4C). When 6-hydroxylation of chlorzoxazone was measured in the cell homogenates, a similar decrease was seen in activities as monitored on the protein level. By contrast, cells transfected with CYP2E1*3 cDNA had apoprotein levels and rates of chlorzoxazone ...
Characterization of chaperonin 10 (Cpn10)
... the E. histolytica gene encoding Cpn10 Searches of preliminary data generated by the E. histolytica genome project at the Sanger Institute revealed several clones with sequence similarity to the human Cpn10 protein sequence. PCR primers based on these clones, when used in PCR reactions on E. histoly ...
... the E. histolytica gene encoding Cpn10 Searches of preliminary data generated by the E. histolytica genome project at the Sanger Institute revealed several clones with sequence similarity to the human Cpn10 protein sequence. PCR primers based on these clones, when used in PCR reactions on E. histoly ...
Genetic Codes with No Dedicated Stop Codon: Context
... here. Surprisingly, in two of these species, we find efficient translation of all 64 codons as standard amino acids and recognition of either one or all three stop codons. How, therefore, does the translation machinery interpret a ‘‘stop’’ codon? We provide evidence, based on ribosomal profiling and ...
... here. Surprisingly, in two of these species, we find efficient translation of all 64 codons as standard amino acids and recognition of either one or all three stop codons. How, therefore, does the translation machinery interpret a ‘‘stop’’ codon? We provide evidence, based on ribosomal profiling and ...
of Lactobacillus pentosus
... While most research is focused on the regulation of the metabolizing genes in these organisms, not much is known about the transport of xylose into the celi. Hastrup (1988) showed that beside the xylR, xylA and xylB genes, two additional genes belonged to the 5. subtilis xylose regulon, the xyn5 and ...
... While most research is focused on the regulation of the metabolizing genes in these organisms, not much is known about the transport of xylose into the celi. Hastrup (1988) showed that beside the xylR, xylA and xylB genes, two additional genes belonged to the 5. subtilis xylose regulon, the xyn5 and ...
Gene Name
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
... frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleas ...
Finishing the Human Genome
... •Need to know at least the beginning and end of DNA sequence •These flanking regions have to be unique to strand interested in amplifying •Region of interest can be present in as little as one copy •Enough DNA in 0.1 microliter of human saliva to use PCR ...
... •Need to know at least the beginning and end of DNA sequence •These flanking regions have to be unique to strand interested in amplifying •Region of interest can be present in as little as one copy •Enough DNA in 0.1 microliter of human saliva to use PCR ...
WHITE PANICLE1, a Val-tRNA Synthetase
... and 4.5S rRNA and displayed a virescent seedling phenotype. RH22 indirectly affected ribosome assembly due to its role in rRNA metabolism (Chi et al., 2012). The null mutant of AtObgC (a protein that could coprecipitate with 23S rRNA) resulted in embryo lethality, and knocking down AtObgC with RNAi ...
... and 4.5S rRNA and displayed a virescent seedling phenotype. RH22 indirectly affected ribosome assembly due to its role in rRNA metabolism (Chi et al., 2012). The null mutant of AtObgC (a protein that could coprecipitate with 23S rRNA) resulted in embryo lethality, and knocking down AtObgC with RNAi ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... 1983). Since the xylE gene comes from the centre of an operon it lacks its own promoter and so to achieve efficient expression, heterologous promoters tac and trp from E. coli, and a S P 0 2 Bacillus phage promoter, all of which are known to function in B. subtilis (Penschke et al., 1-985;Williams e ...
... 1983). Since the xylE gene comes from the centre of an operon it lacks its own promoter and so to achieve efficient expression, heterologous promoters tac and trp from E. coli, and a S P 0 2 Bacillus phage promoter, all of which are known to function in B. subtilis (Penschke et al., 1-985;Williams e ...
Complete
... to manipulate and probe biological molecules. In this thesis, we first discuss how microfabricated Brownian ratchets are used for separation of molecules, including nucleic acids. More specifically, our contribution to this field includes clarifying the role of particle size in the ratchet array ope ...
... to manipulate and probe biological molecules. In this thesis, we first discuss how microfabricated Brownian ratchets are used for separation of molecules, including nucleic acids. More specifically, our contribution to this field includes clarifying the role of particle size in the ratchet array ope ...
... Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder influenced by interactions between genetic and environmental factors. Epigenetics conveys specific environmental influences into phenotypic traits through a variety of mechanisms that are often installed in early life, then persist in differenti ...
Gene Detection Systems Catalog
... the applications of our products. We routinely assist our customers regarding the design of their experiments and the solving of their application problems to help researchers reach their goals faster and with greater success. Gene Link customers are Universities, Research Institutes, Pharmaceutical ...
... the applications of our products. We routinely assist our customers regarding the design of their experiments and the solving of their application problems to help researchers reach their goals faster and with greater success. Gene Link customers are Universities, Research Institutes, Pharmaceutical ...
Comparative sequence analysis of the long repeat regions and
... DNA sequence determination. Four plasmid-cloned fragments of HSV-2 strain HG52 DNA were used for sequence determination: BamHIf(cloned in pAT153; Whitton et al., 1983), BamH1 g (cloned in pAT153, from A. J. Davison) and BamHI p and BamHl c (cloned in pUCI8 for this study). HSV-2 inserts were recover ...
... DNA sequence determination. Four plasmid-cloned fragments of HSV-2 strain HG52 DNA were used for sequence determination: BamHIf(cloned in pAT153; Whitton et al., 1983), BamH1 g (cloned in pAT153, from A. J. Davison) and BamHI p and BamHl c (cloned in pUCI8 for this study). HSV-2 inserts were recover ...
Perspectives in Diabetes Glucokinase Gene Structure
... blot-transfer analysis of RNA from liver and insulinomatissue (25). Our experiment indicated that the glucokinase mRNA in the p-cell is -200 nucleotides longer than that in the liver. A glucokinase cDNA from a rat insulinoma cDNA library was then isolated, and its sequence was compared with the sequ ...
... blot-transfer analysis of RNA from liver and insulinomatissue (25). Our experiment indicated that the glucokinase mRNA in the p-cell is -200 nucleotides longer than that in the liver. A glucokinase cDNA from a rat insulinoma cDNA library was then isolated, and its sequence was compared with the sequ ...
DNA cytosine methylation in plant development
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... In Figure 2(a), a set of 12 nucleotides ‘ACGCTTACGCAT’ is a sample sequence. The subsequence ‘ACGCTT’ shown between 1 and 6 indicates the first six bases of the sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line l ...
... In Figure 2(a), a set of 12 nucleotides ‘ACGCTTACGCAT’ is a sample sequence. The subsequence ‘ACGCTT’ shown between 1 and 6 indicates the first six bases of the sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line l ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.