A natural chimeric yeast containing genetic material from three species
... and 5h-CGGCTCTAGAGACCACGATATG CACCAGGCAG-3h, which possess terminal XbaI restriction sites in addition to four arbitrary bases, thus allowing restriction digestion. Genomic DNA was prepared from liquid yeast ...
... and 5h-CGGCTCTAGAGACCACGATATG CACCAGGCAG-3h, which possess terminal XbaI restriction sites in addition to four arbitrary bases, thus allowing restriction digestion. Genomic DNA was prepared from liquid yeast ...
Bacteriophage A cloning system for the construction of
... phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containing single recognition sequences for the restriction enzymes B ...
... phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containing single recognition sequences for the restriction enzymes B ...
Analysis and nucleotide sequence of an origin of DNA replication in
... Restreaking on minimal plates with Ap yielded 16 candidates. From these the plasmid DNA was prepared from 3 ml overnight cultures and retransformed to A. calcoaceticus BD413 trpE. The transformants were plated on LB-Ap plates and minimal medium Ap plates. Four plasmids yielded roughly the same numbe ...
... Restreaking on minimal plates with Ap yielded 16 candidates. From these the plasmid DNA was prepared from 3 ml overnight cultures and retransformed to A. calcoaceticus BD413 trpE. The transformants were plated on LB-Ap plates and minimal medium Ap plates. Four plasmids yielded roughly the same numbe ...
Gene Section MYB (v-myb myeloblastosis viral oncogene homolog (avian))
... progenitor cells. Knockdown of MYB in leukaemic cells and estrogen receptor positive breast cancer cells, where it is highly expressed, significantly slows down cell proliferation. Overexpressed or activated MYB suppresses normal differentiation and promotes leukaemic transformation. However, MYB al ...
... progenitor cells. Knockdown of MYB in leukaemic cells and estrogen receptor positive breast cancer cells, where it is highly expressed, significantly slows down cell proliferation. Overexpressed or activated MYB suppresses normal differentiation and promotes leukaemic transformation. However, MYB al ...
Slides
... CNVs in human genomes by Prof. Chris Ponting The Future of CNVs: Sequence base resolution and links to human disea Professor Evan Eichler – University of Washington • You will need the Stanford name and password (stanford, member) in order to watch this course off campus. ...
... CNVs in human genomes by Prof. Chris Ponting The Future of CNVs: Sequence base resolution and links to human disea Professor Evan Eichler – University of Washington • You will need the Stanford name and password (stanford, member) in order to watch this course off campus. ...
TCF11/Nrf1 overexpression increases the intracellular
... the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transfected using calcium phosphate and including CAT as intern ...
... the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transfected using calcium phosphate and including CAT as intern ...
Point Mutation in Intron Sequence Causes Altered Carboxyl
... strain has a point mutation (alanine to valine) in its ligand binding domain in codon 375 resulting in markedly reduced binding affinity for TCDD (Ema et al., 1994; Poland et al., 1994); the apparent number of binding sites is also lower than that in C57BL/6 mice (Okey et al., 1989). The situation i ...
... strain has a point mutation (alanine to valine) in its ligand binding domain in codon 375 resulting in markedly reduced binding affinity for TCDD (Ema et al., 1994; Poland et al., 1994); the apparent number of binding sites is also lower than that in C57BL/6 mice (Okey et al., 1989). The situation i ...
letters - Centre for Social Evolution
... sex-determining function by RNAi knockdown experiments. Only csd and the new fem gene, located 12 kb upstream of csd, have sex determination function (Fig. 1b). RNAi-induced knockdowns of fem in females result in a developmental switch to entire male head differentiation (Fig. 1b), whereas knockdown ...
... sex-determining function by RNAi knockdown experiments. Only csd and the new fem gene, located 12 kb upstream of csd, have sex determination function (Fig. 1b). RNAi-induced knockdowns of fem in females result in a developmental switch to entire male head differentiation (Fig. 1b), whereas knockdown ...
Analysis of clones carrying repeated DNA sequences in two YAC
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
... coordinates of the YAC clones which hybridized to chloroplast DNA with the coordinates of clones which had been mapped previously on to chromosome 4 and the top half of chromosome 5 using RFLP markers as probes (Hwang eta/., 1991; Schmidt etal., unpublished results). One-hundred-and-ninety-five clon ...
PowerPoint Slides - Evo-Ed
... • Promoters: DNA sequences that bind RNA polymerase and transcription factors. Promoters initiate transcription (turn on genes) for production of mRNA; usually located upstream of the gene it controls. • Operators: regions of DNA associated with promoters that bind regulatory proteins to either prom ...
... • Promoters: DNA sequences that bind RNA polymerase and transcription factors. Promoters initiate transcription (turn on genes) for production of mRNA; usually located upstream of the gene it controls. • Operators: regions of DNA associated with promoters that bind regulatory proteins to either prom ...
Repair of Site-Specific DNA Double-Strand Breaks in
... together by cohesins or actively find each other via the SMC5/6 complex (Watanabe et al., 2009). HR is initiated by the 59 to 39 resection of DNA ends at the DSB to produce single-stranded overhanging 39 ends that invade a DNA duplex containing a homologous sequence (Symington and Gautier, 2011). Usi ...
... together by cohesins or actively find each other via the SMC5/6 complex (Watanabe et al., 2009). HR is initiated by the 59 to 39 resection of DNA ends at the DSB to produce single-stranded overhanging 39 ends that invade a DNA duplex containing a homologous sequence (Symington and Gautier, 2011). Usi ...
Rosalind Elsie Franklin Physicist www.AssignmentPoint.com
... Wilkins penned the following: "I think you're a couple of old rogues, but you may well have something". ...
... Wilkins penned the following: "I think you're a couple of old rogues, but you may well have something". ...
Julio`s MCB - Digital Access to Scholarship at Harvard
... the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediated transcription of Mfrn1. Furthermore, ChIP assays demonstrate switching from GATA-2 to GATA-1 at these eleme ...
... the expression of Mfrn1. Mutagenesis of individual GATA-1 binding cis elements (GBE) demonstrated that at least two of the three GBE within this CRM are functionally required for GATA-mediated transcription of Mfrn1. Furthermore, ChIP assays demonstrate switching from GATA-2 to GATA-1 at these eleme ...
bacterial plasmids - Acta Medica Medianae
... canal, formed in this way, allows DNA transfer, or through the pilus itself, which is wide enough to enable passing of single chain DNA, or we have fusion of cell membranes at the contact spot which leads to creation of transmembrane pore as a passage for DNA. Initiation is catalyzed most frequently ...
... canal, formed in this way, allows DNA transfer, or through the pilus itself, which is wide enough to enable passing of single chain DNA, or we have fusion of cell membranes at the contact spot which leads to creation of transmembrane pore as a passage for DNA. Initiation is catalyzed most frequently ...
Identification of genes that are associated with DNA repeats in
... may lead to phenotypic differences due to differential gene transcription or translation. These SSRs are often part of open reading frames or promoter regions, and variation in the number of repeat units may lead to the switch of expression of surface-exposed components (Dybvig, 1993; Belland et al. ...
... may lead to phenotypic differences due to differential gene transcription or translation. These SSRs are often part of open reading frames or promoter regions, and variation in the number of repeat units may lead to the switch of expression of surface-exposed components (Dybvig, 1993; Belland et al. ...
Distortion of quantitative genomic and expression
... to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 237– 43 964 330) consistently exhibited a small decrease in hybridization intensity with addition of Cot-1 DNA (Table 1, reactions 7–12) with a 0.84- to 0.92-fold decrease in hybridizati ...
... to the composition of repetitive sequences surrounding these sc intervals. An sc probe from HOXB1 (chr 17: 43 964 237– 43 964 330) consistently exhibited a small decrease in hybridization intensity with addition of Cot-1 DNA (Table 1, reactions 7–12) with a 0.84- to 0.92-fold decrease in hybridizati ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects them from exonuclease resection, and facilitates end-to-end interaction (Van Dyck et al., 1999). Furthermore, capture of the second DNA end in homo ...
... important for the binding with ssDNA by RAD52 (Hays et al., 1998; Shinohara et al., 1998; Jackson et al., 2002). hRAD52 binds directly to DSBs, protects them from exonuclease resection, and facilitates end-to-end interaction (Van Dyck et al., 1999). Furthermore, capture of the second DNA end in homo ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... activity at VYCV target sites (>20% indel for 25/37 (68%) of sites) (Fig. 2h), although this activity was somewhat lower than at TYCV sites, consistent with the in vitro cleavage assay. We also tested the RR variant in murine Neuro2a cells (Supplementary Fig. 1d) and observed high rates of editing ( ...
... activity at VYCV target sites (>20% indel for 25/37 (68%) of sites) (Fig. 2h), although this activity was somewhat lower than at TYCV sites, consistent with the in vitro cleavage assay. We also tested the RR variant in murine Neuro2a cells (Supplementary Fig. 1d) and observed high rates of editing ( ...
reproductive cell fate transition in plants - Development
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
... at 18-20°C in a plant growth chamber or greenhouse, except for the mutants ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP ...
Transposon stability and a role for conjugational transfer in adaptive mutability
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
... (16–21). Moreover, even lacI33 Lac⫺ cells, products of conjugal transfer to scavenger, frequently had become TetS. About 10% of the late-appearing Lac⫹ revertants that are TetS have lost the transposon DNA, most often precisely, and an equal number show evidence of amplification of the Lac兾Tn geneti ...
Variant Effect Predictor Web interface Documentation
... Uniprot - add identifiers for translated protein products from three UniProt -related databases (SWISSPROT, TREMBL and UniParc). Equivalent to --uniprot HGVS - generate HGVS identifiers for your input variants relative to the transcript coding sequence (HGVSc) and the protein sequence (HGVSp). Equiv ...
... Uniprot - add identifiers for translated protein products from three UniProt -related databases (SWISSPROT, TREMBL and UniParc). Equivalent to --uniprot HGVS - generate HGVS identifiers for your input variants relative to the transcript coding sequence (HGVSc) and the protein sequence (HGVSp). Equiv ...
Role of virulence factors, cell components and adhesion in
... wild-type and mutant strains were equally potent iNOS inducers as only minor di¡erences were observed (Fig. 2). Thus, by use of these mutant strains we have for the ¢rst time clearly shown that iNOS induction in J774A.1 is independent of the cag PAI. Our result is supported by other investigators wh ...
... wild-type and mutant strains were equally potent iNOS inducers as only minor di¡erences were observed (Fig. 2). Thus, by use of these mutant strains we have for the ¢rst time clearly shown that iNOS induction in J774A.1 is independent of the cag PAI. Our result is supported by other investigators wh ...
M.Sc., Biotechnology - Alagappa University
... of diseases and in designing appropriate therapies. Currently the smart use of various biochemical laboratory tests have become an integral part of disease diagnosis and monitoring the treatment. A high understanding of biochemistry and of other related basic disciplines such as cell biology, molecu ...
... of diseases and in designing appropriate therapies. Currently the smart use of various biochemical laboratory tests have become an integral part of disease diagnosis and monitoring the treatment. A high understanding of biochemistry and of other related basic disciplines such as cell biology, molecu ...
FLUORESCENT PROTEIN IN Escherichia coli
... The mode of action of antibacterial activity of AMPs have mainly been studied through interactions between AMPs and model membrane systems, but studies have also been conducted using microbial cells with membrane potential sensitive dyes and flurescently labeled peptides. These experiments have made ...
... The mode of action of antibacterial activity of AMPs have mainly been studied through interactions between AMPs and model membrane systems, but studies have also been conducted using microbial cells with membrane potential sensitive dyes and flurescently labeled peptides. These experiments have made ...
Pleiotropic effects of the mouse lethal yellow (Ay) mutation
... We have previously described the isolation and structure of cosmid clones that constitute a 60 kb contig containing agouti exons 1B, 1C, 2, 3 and 4 (Miller et al., 1993) (The agouti exon nomenclature used in the present work differs from that used previously; the rationale is summarized in Fig. 1). ...
... We have previously described the isolation and structure of cosmid clones that constitute a 60 kb contig containing agouti exons 1B, 1C, 2, 3 and 4 (Miller et al., 1993) (The agouti exon nomenclature used in the present work differs from that used previously; the rationale is summarized in Fig. 1). ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.