S-nitrosothiols regulate nitric oxide production and storage in plants
... Author contributions: L.F. performed the enzymatic assays, determination of nitrate, amino acid content and NO emission, analysis of growth vigor, and wrote the manuscript. M.J.S. was under supervision of G.J.L. and performed biotin switch technique and western blot assays together with L.F. Both L. ...
... Author contributions: L.F. performed the enzymatic assays, determination of nitrate, amino acid content and NO emission, analysis of growth vigor, and wrote the manuscript. M.J.S. was under supervision of G.J.L. and performed biotin switch technique and western blot assays together with L.F. Both L. ...
Serine racemases from barley, Hordeum vulgare L., and other plant
... mammalian serine racemases belong to the fold type II PLP enzymes and that Lys62 which binds to PLP, Phe61 and Gly241, which sandwich the PLP ring, Ser315, the side-chain of which is hydrogen-bonded to the pyridium nitrogen of PLP, and Asn89, which stabilizes the 3’ oxygen of PLP by a hydrogen bond ...
... mammalian serine racemases belong to the fold type II PLP enzymes and that Lys62 which binds to PLP, Phe61 and Gly241, which sandwich the PLP ring, Ser315, the side-chain of which is hydrogen-bonded to the pyridium nitrogen of PLP, and Asn89, which stabilizes the 3’ oxygen of PLP by a hydrogen bond ...
Regulation of thiamine synthesis in Saccharomyces cerevisiae for
... cassette consisting of loxP–kanMX–loxP. The resulting PCR product was composed of the kanMX gene, loxP sites and homologous regions for the target gene in FMME-002. The PCR products were transformed into yeast by the LiAc/SS Carrier DNA/PEG method. Homologous recombination in yeast led to the deleti ...
... cassette consisting of loxP–kanMX–loxP. The resulting PCR product was composed of the kanMX gene, loxP sites and homologous regions for the target gene in FMME-002. The PCR products were transformed into yeast by the LiAc/SS Carrier DNA/PEG method. Homologous recombination in yeast led to the deleti ...
The relevance of carbon dioxide metabolism in
... have demonstrated that CO2 and sodium bicarbonate stimulate growth and exert a marked influence on the metabolic activities of S. thermophilus. In analyses of the bacterial genome of two strains (Bolotin et al., 2004; Hols et al., 2005), there has been observation of two main anaplerotic pathways th ...
... have demonstrated that CO2 and sodium bicarbonate stimulate growth and exert a marked influence on the metabolic activities of S. thermophilus. In analyses of the bacterial genome of two strains (Bolotin et al., 2004; Hols et al., 2005), there has been observation of two main anaplerotic pathways th ...
Interrogating the Function of Metazoan Histones using
... for the maintenance of Polycomb target gene repression, demonstrating that histone residues can perform an essential function in gene regulation. These results underscore the essential roles played by these two histone residues in gene expression and animal development. Finally, in contrast to curre ...
... for the maintenance of Polycomb target gene repression, demonstrating that histone residues can perform an essential function in gene regulation. These results underscore the essential roles played by these two histone residues in gene expression and animal development. Finally, in contrast to curre ...
Unveiling the physiological and molecular basis of Mycobacterium
... triggered by cell-density dependent Quorum-Sensing (QS) events, exhibit heterogeneous and stress-tolerant populations that thrive under inhospitable niches and display recalcitrant characters, such as increased xenobiotic tolerance and resistance to host’s immune response. M. tuberculosis infections ...
... triggered by cell-density dependent Quorum-Sensing (QS) events, exhibit heterogeneous and stress-tolerant populations that thrive under inhospitable niches and display recalcitrant characters, such as increased xenobiotic tolerance and resistance to host’s immune response. M. tuberculosis infections ...
Kinetic Role of Helix Caps in Protein Folding Is Context
... conformation with good H-bonds and no steric clashes. If that was not possible without significant backbone motion (e.g., when trying to provide a cap-box H-bond for the N-cap of helix 4), the variant was not tried experimentally. RESULTS The sequence substitutions used in this study were made in on ...
... conformation with good H-bonds and no steric clashes. If that was not possible without significant backbone motion (e.g., when trying to provide a cap-box H-bond for the N-cap of helix 4), the variant was not tried experimentally. RESULTS The sequence substitutions used in this study were made in on ...
Iron mediated methylthiolation of tRNA as a regulator of operon
... When the S30 extracts from broth-grown E. coli MRE 600 was programmed with MS2 RNA , 36 pp moles valine were incorporated per 150 pi reaction mixture after 30 min. The S30 extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles ...
... When the S30 extracts from broth-grown E. coli MRE 600 was programmed with MS2 RNA , 36 pp moles valine were incorporated per 150 pi reaction mixture after 30 min. The S30 extract from the bacteria grown in broth containing ovotransferrin, however, incorporated nearly twice this amount, 70 pp moles ...
Aspects of Reductive Explanation in Biological Science: Intrinsicality
... the increased rhythmic contraction of the heart by the cellular mechanisms that process adrenergic hormones, then the explanation requires (at least implicitly) an earlier time at which hormones are interacting with cellular receptors and a later time when the altered rhythmic contraction obtains. T ...
... the increased rhythmic contraction of the heart by the cellular mechanisms that process adrenergic hormones, then the explanation requires (at least implicitly) an earlier time at which hormones are interacting with cellular receptors and a later time when the altered rhythmic contraction obtains. T ...
MPN+, a putative catalytic motif found in a subset of MPN domain
... fact that invariably all observed orthologs of MPN+ proteins also belong to the MPN+ class. For instance, the proteasome lid complex from all eukaryotes contains one MPN+ protein (Rpn11/S13) and one plain MPN protein (Rpn8/S12). The same is true for the analogous CSN complex in multicellular eukaryo ...
... fact that invariably all observed orthologs of MPN+ proteins also belong to the MPN+ class. For instance, the proteasome lid complex from all eukaryotes contains one MPN+ protein (Rpn11/S13) and one plain MPN protein (Rpn8/S12). The same is true for the analogous CSN complex in multicellular eukaryo ...
Synonymous codons are not the same with
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
... result to appreciate this notion of translation speed difference among the synonymous codons 8. So the difference among synonymous codons with regard to translational speed was not conclusive from any of the previously performed experiments. In this context, Yu et al. 9 have recently provided conclu ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... the most thoroughly studied class of nonhistone chromosomal proteins. This family of relatively low molecular weight proteins (mol wt <30,000) can be extracted from chromatin by low salt or with dilute acids, and characteristically contain a large number of basic and acidic amino acids distributed i ...
... the most thoroughly studied class of nonhistone chromosomal proteins. This family of relatively low molecular weight proteins (mol wt <30,000) can be extracted from chromatin by low salt or with dilute acids, and characteristically contain a large number of basic and acidic amino acids distributed i ...
Sequence and evolutionary analysis of the human trypsin subfamily
... substrate, a peptide bond, but their specificities vary. The most widely used system for classification of peptidases is the MEROPS Clan System, where enzymes are first sorted into ‘‘clans’’ (sometimes referred to as superfamilies) based on evidence of evolutionary relationship [1,7,8]. Evidence for ...
... substrate, a peptide bond, but their specificities vary. The most widely used system for classification of peptidases is the MEROPS Clan System, where enzymes are first sorted into ‘‘clans’’ (sometimes referred to as superfamilies) based on evidence of evolutionary relationship [1,7,8]. Evidence for ...
Functional genomics analysis of the effects of co- decarboxylase/ornithine decarboxylase
... intervention, polyamine biosynthesis was completely restrained via the inhibition of both catalytic sites of PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by vari ...
... intervention, polyamine biosynthesis was completely restrained via the inhibition of both catalytic sites of PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by vari ...
Landick R, Yanofsky C. 1987. Transcription
... transcribe the remainder of the operon. Alternatively, if there is an adequate level of charged tRNATrp, the ribosome translating the leader peptide coding region would continue beyond the Trp codons to the end of the coding region and then either remain at the translation stop codon (as shown in Fi ...
... transcribe the remainder of the operon. Alternatively, if there is an adequate level of charged tRNATrp, the ribosome translating the leader peptide coding region would continue beyond the Trp codons to the end of the coding region and then either remain at the translation stop codon (as shown in Fi ...
4. characterisation of novel proteins
... Monsanto Company has developed a genetically modified (GM) soybean, line MON87769, which produces stearidonic acid (SDA), an omega-3 fatty acid. The modification was achieved through the introduction of genes encoding two enzymes involved in fatty acid metabolism, delta-6 desaturase from the plant P ...
... Monsanto Company has developed a genetically modified (GM) soybean, line MON87769, which produces stearidonic acid (SDA), an omega-3 fatty acid. The modification was achieved through the introduction of genes encoding two enzymes involved in fatty acid metabolism, delta-6 desaturase from the plant P ...
Production of long chain alcohols and alkanes upon coexpression of
... While acyl-ACPs inhibit several enzymes in type-II FAS pathways,20,21 they may not inhibit type-I FAS enzymes where the reaction center is contained in a single multi-domain polypeptide,5 although acyl-CoAs then may become the potential repressors.22 Most recently, when this manuscript is under revi ...
... While acyl-ACPs inhibit several enzymes in type-II FAS pathways,20,21 they may not inhibit type-I FAS enzymes where the reaction center is contained in a single multi-domain polypeptide,5 although acyl-CoAs then may become the potential repressors.22 Most recently, when this manuscript is under revi ...
148 - Blue Ridge Institute for Medical Research
... one of the most important drug targets over the past two decades. Imatinib was approved by the United States FDA for the treatment of chronic myelogenous leukemia in 2001; this small molecule inhibits the BCR-Abl protein kinase oncoprotein that results from the formation of the Philadelphia chromoso ...
... one of the most important drug targets over the past two decades. Imatinib was approved by the United States FDA for the treatment of chronic myelogenous leukemia in 2001; this small molecule inhibits the BCR-Abl protein kinase oncoprotein that results from the formation of the Philadelphia chromoso ...
Protein Similarity Score - Santa Clara Law Digital Commons
... However, in many cases substantial variations between sequences are tolerated without giving rise to any substantial difference in threedimensional conformation or function.1 2 In naturally-occurring proteins, for instance, sequence similarity between two proteins can be used to infer an evolutionar ...
... However, in many cases substantial variations between sequences are tolerated without giving rise to any substantial difference in threedimensional conformation or function.1 2 In naturally-occurring proteins, for instance, sequence similarity between two proteins can be used to infer an evolutionar ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... Asp. niger3) were cloned and it was discovered that their structures were the same. Glucoamylases are especially useful in saccharifying starch in commercial alcohol production. It is unfortunate for this commer- ...
... Asp. niger3) were cloned and it was discovered that their structures were the same. Glucoamylases are especially useful in saccharifying starch in commercial alcohol production. It is unfortunate for this commer- ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... explanation for many of the protein’s properties. Although many thousands of homologs of AraC exist and regulate many diverse operons in response to many different inducers or physiological states, homologs that regulate arabinosecatabolizing genes in response to arabinose were identified. The seque ...
... explanation for many of the protein’s properties. Although many thousands of homologs of AraC exist and regulate many diverse operons in response to many different inducers or physiological states, homologs that regulate arabinosecatabolizing genes in response to arabinose were identified. The seque ...
An Investigation of Acetobacter aceti N5
... mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surve ...
... mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was found to be significantly more thermostable than EcPurE over the entire pH range surve ...
Nitrate reductase activity in chicory roots
... When the nitrate assimilatory pathway occurs in the roots, high amounts of photosynthates must be imported and oxidized to provide the required reductants, energy and carbon skeletons. It has been assumed that the mechanisms involved in the control of nitrate reduction were similar in leaves and roo ...
... When the nitrate assimilatory pathway occurs in the roots, high amounts of photosynthates must be imported and oxidized to provide the required reductants, energy and carbon skeletons. It has been assumed that the mechanisms involved in the control of nitrate reduction were similar in leaves and roo ...
Protein Structure Prediction Based on Neural Networks
... has several advantages over the experimental methods, its accuracy is templateintensive. This method assumes that the assignments from the chemical properties to the spatial regions between the query protein and the template protein are the same. However, this is not the case in reality, especially ...
... has several advantages over the experimental methods, its accuracy is templateintensive. This method assumes that the assignments from the chemical properties to the spatial regions between the query protein and the template protein are the same. However, this is not the case in reality, especially ...
Research in Microbiology
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...