6 - rguhs
... 1. Stosz. et.al., (1998) have shown that production of the enzyme glucose oxidase was involved in the biological control of Verticillium wilt by Talaromyces flavus. In this study, the location of glucose antibody by immunostaining revealed that glucose oxidase was both intracellular and extracellula ...
... 1. Stosz. et.al., (1998) have shown that production of the enzyme glucose oxidase was involved in the biological control of Verticillium wilt by Talaromyces flavus. In this study, the location of glucose antibody by immunostaining revealed that glucose oxidase was both intracellular and extracellula ...
The SRA gen - uri=dna.kdna.ucla
... primers specific to ESAG 7/6, which allowed the discrimination between the different ES transcripts (data not shown). Overall these results suggested that activation of the ETat 1.10 ES is required to generate R clones and that in the absence of NHS the ETat 1.10 ES is counterselected. In These Clon ...
... primers specific to ESAG 7/6, which allowed the discrimination between the different ES transcripts (data not shown). Overall these results suggested that activation of the ETat 1.10 ES is required to generate R clones and that in the absence of NHS the ETat 1.10 ES is counterselected. In These Clon ...
PDF
... grow at 90°C, have S%reducing, saccharolytic metabolisms, and are the most slowly evolving organisms within the their domain [2,3]. T. maritima differs from the archaea, however, in that it couples glucose oxidation to H 2 production via a conventional Embden-Meyerhof-Parnas pathway, pyruvate ferred ...
... grow at 90°C, have S%reducing, saccharolytic metabolisms, and are the most slowly evolving organisms within the their domain [2,3]. T. maritima differs from the archaea, however, in that it couples glucose oxidation to H 2 production via a conventional Embden-Meyerhof-Parnas pathway, pyruvate ferred ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... okadaic acid and microcystin-LR, as well as diversity in their phosphorylated substrate specificities. Bioinformatics complemented cell biology also uncovered unique subcellular localizations for each enzyme, with AtSLP1, 2 and AtRLPH2 being chloroplastic, mitochondrial and nuclear / cytosolic, resp ...
... okadaic acid and microcystin-LR, as well as diversity in their phosphorylated substrate specificities. Bioinformatics complemented cell biology also uncovered unique subcellular localizations for each enzyme, with AtSLP1, 2 and AtRLPH2 being chloroplastic, mitochondrial and nuclear / cytosolic, resp ...
Crystallization and X Ray Diffraction Data Analyses of the Enzyme
... formimine glutamase (EC 3.5.3.8). This complete pathway drives the conversion of histidine into glutamate, which in turn can be dea i ated i to α-ketoglutarate, allowing its complete oxidation. Urocanate hydratase, the second enzyme of the histidine-glutamate pathway, acts on the conversion of uroca ...
... formimine glutamase (EC 3.5.3.8). This complete pathway drives the conversion of histidine into glutamate, which in turn can be dea i ated i to α-ketoglutarate, allowing its complete oxidation. Urocanate hydratase, the second enzyme of the histidine-glutamate pathway, acts on the conversion of uroca ...
Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using
... 10-mM imidazole, pH 7.0) per gram (wet weight) of cells. Lysozyme (1 mg/mL) was added to the resuspended cells, and the cell suspension was incubated for 30 min on ice before disruption by sonication (Sonifier 450, Branson, Danbury, CT, USA; output four, six times for 10 s, constant duty) in an ice ...
... 10-mM imidazole, pH 7.0) per gram (wet weight) of cells. Lysozyme (1 mg/mL) was added to the resuspended cells, and the cell suspension was incubated for 30 min on ice before disruption by sonication (Sonifier 450, Branson, Danbury, CT, USA; output four, six times for 10 s, constant duty) in an ice ...
as a PDF
... the medium. It was therefore surprising to discover that some bacteria, including Lactococcus lactis, had the capacity for respiration-based growth when a heme source and oxygen were present in the medium (12, 16, 24, 47). In L. lactis, respiration growth results in improved biomass, higher pH, and ...
... the medium. It was therefore surprising to discover that some bacteria, including Lactococcus lactis, had the capacity for respiration-based growth when a heme source and oxygen were present in the medium (12, 16, 24, 47). In L. lactis, respiration growth results in improved biomass, higher pH, and ...
Cloning of adiponectin receptors that mediate antidiabetic metabolic
... letters to nature cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocy ...
... letters to nature cyclic AMP, cyclic GMP and intracellular calcium levels (Supplementary Fig. 2c, d). In contrast, expression of AdipoR1 enhanced increases in PPAR-a ligand activity by both globular and full-length adiponectin (Supplementary Fig. 2e). Expression of AdipoR1 or AdipoR2 in C2C12 myocy ...
Fastness in Mycobacterium tuberculosis
... site-directed mutagenesis revealed that phosphorylation of KasB occurred at Thr334 and Thr336 both in vitro and in mycobacteria. Isogenic strains of M. tuberculosis with either a deletion of the kasB gene or a kasB_T334D/T336D allele, mimicking constitutive phosphorylation of KasB, were constructed ...
... site-directed mutagenesis revealed that phosphorylation of KasB occurred at Thr334 and Thr336 both in vitro and in mycobacteria. Isogenic strains of M. tuberculosis with either a deletion of the kasB gene or a kasB_T334D/T336D allele, mimicking constitutive phosphorylation of KasB, were constructed ...
Time-Resolved Transcriptome Analysis of Bacillus subtilis
... have a key regulatory role, by different mechanisms, in both grampositive and gram-negative bacteria. The intracellular BCAA concentrations appear to serve as a gauge of the nutrient conditions, that is, a pacemaker of the synthesis of proteins and membranes. Thus BCAAs seem to be widely used by bac ...
... have a key regulatory role, by different mechanisms, in both grampositive and gram-negative bacteria. The intracellular BCAA concentrations appear to serve as a gauge of the nutrient conditions, that is, a pacemaker of the synthesis of proteins and membranes. Thus BCAAs seem to be widely used by bac ...
Conclusion - Federal Register of Legislation
... Food Standards Australia New Zealand (FSANZ) received an Application from Syngenta Seeds Pty Ltd (the Applicant) on 10 October 2007. The Applicant has requested an amendment to the Australia New Zealand Food Standards Code (the Code), specifically to Standard 1.5.2 – Food produced using Gene Technol ...
... Food Standards Australia New Zealand (FSANZ) received an Application from Syngenta Seeds Pty Ltd (the Applicant) on 10 October 2007. The Applicant has requested an amendment to the Australia New Zealand Food Standards Code (the Code), specifically to Standard 1.5.2 – Food produced using Gene Technol ...
Constitutively Active CaMKKa Stimulates Skeletal Muscle
... cellular and metabolic processes in muscle, including the regulation of contractile activity and glucose uptake. Importantly, studies have shown that stimulation of isolated rodent muscles with low doses of the sarcoplasmic reticulum Ca2+ release agents, caffeine or N-(6-amino-hexyl)-5-chloro-1-napt ...
... cellular and metabolic processes in muscle, including the regulation of contractile activity and glucose uptake. Importantly, studies have shown that stimulation of isolated rodent muscles with low doses of the sarcoplasmic reticulum Ca2+ release agents, caffeine or N-(6-amino-hexyl)-5-chloro-1-napt ...
SURVEY AND SUMMARY A profusion of upstream open reading
... peptide-ribosome interaction (12); perhaps involving the internal wall of the ribosomal peptide exit tunnel. However, polyamines also influence ribosome pausing near the termination codon on non-inhibitory variants of the uORF peptide (11). Small molecule regulation via effects on termination stalling ...
... peptide-ribosome interaction (12); perhaps involving the internal wall of the ribosomal peptide exit tunnel. However, polyamines also influence ribosome pausing near the termination codon on non-inhibitory variants of the uORF peptide (11). Small molecule regulation via effects on termination stalling ...
Regulation of Translation by Essential Amino Acids and Glucose in
... To everyone who has contributed to this chapter of my life - please consider this is the ultimate thank you, as none of it would have happened without the brilliance, support, and good humor of each one of you. Most significantly, to my advisor Dr. John Cant – thank you for providing me with every o ...
... To everyone who has contributed to this chapter of my life - please consider this is the ultimate thank you, as none of it would have happened without the brilliance, support, and good humor of each one of you. Most significantly, to my advisor Dr. John Cant – thank you for providing me with every o ...
Mechanistic Studies Of Drug Resistance Conferred By An ABC
... First and for most, I would like to thank my mentor Dr. Parjit Kaur, without whom this work could never be done. I am grateful for her well-rounded guidance and support throughout all these years’ Ph.D studies. Her understanding, sincerity and patience have encouraged me to overcome the obstacles an ...
... First and for most, I would like to thank my mentor Dr. Parjit Kaur, without whom this work could never be done. I am grateful for her well-rounded guidance and support throughout all these years’ Ph.D studies. Her understanding, sincerity and patience have encouraged me to overcome the obstacles an ...
Protein Feature Identification
... Protein Annotation • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, l ...
... Protein Annotation • Objective - identify and describe all the physico-chemical, functional and structural properties of a protein including its sequence, accession #, mass, pI, absorptivity, solubility, active sites, binding sites, reactions, substrates, homologues, function, name(s), abundance, l ...
Beverage Treatment Products Technical Data Sheet Pure
... restricts the quantity to the maximum dose quoted above. Dosages of more than 3.3 lb/1,000 gal (40 g/hl) would lead to the limit values for the specified ingredients to be exceeded. Ensure proper mixing of the must! ...
... restricts the quantity to the maximum dose quoted above. Dosages of more than 3.3 lb/1,000 gal (40 g/hl) would lead to the limit values for the specified ingredients to be exceeded. Ensure proper mixing of the must! ...
NIH Public Access
... The major features of the M. thermoacetica genome are listed in Table 1 and shown in Supplementary Figure S1. The genome consists of a single circular 2,628,784 bp chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative ori ...
... The major features of the M. thermoacetica genome are listed in Table 1 and shown in Supplementary Figure S1. The genome consists of a single circular 2,628,784 bp chromosome that has a GC content of 56 % (Supplementary Figure S1). Base pair one of the chromosome was assigned within the putative ori ...
The Serratia gene cluster encoding biosynthesis of the red antibiotic
... Fig. 1. (a) Comparison of the prodigiosin biosynthetic clusters (pig cluster) from Serratia 39006, Sma 274, and the undecylprodigiosin biosynthetic cluster (red cluster) from Str. coelicolor A3(2). Lines between the Str. coelicolor red and Serratia pig clusters trace the homologue genes relative pos ...
... Fig. 1. (a) Comparison of the prodigiosin biosynthetic clusters (pig cluster) from Serratia 39006, Sma 274, and the undecylprodigiosin biosynthetic cluster (red cluster) from Str. coelicolor A3(2). Lines between the Str. coelicolor red and Serratia pig clusters trace the homologue genes relative pos ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporter SGLT2* J. Dairy Sci. 88:2738–2748
... Scan-Prosite analysis predicted 3 potential N-glycosylation sites positioned at amino acids 250 to 253 (NISS), 306 to 309 (NLTH), and 399 to 402 (NSSS) of bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed ...
... Scan-Prosite analysis predicted 3 potential N-glycosylation sites positioned at amino acids 250 to 253 (NISS), 306 to 309 (NLTH), and 399 to 402 (NSSS) of bSGLT2 (Figure 1). However, it is unclear whether these sites are actually glycosylated, because the in vitro translated product of bSGLT2 showed ...
-portal.org Journal of Molecular Biology
... 0022-2836/$ - see front matter © 2012 Elsevier Ltd. All rights reserved. ...
... 0022-2836/$ - see front matter © 2012 Elsevier Ltd. All rights reserved. ...
Case Study 5 Literature - Department of Chemistry
... GAPDH from T. tenax—The sequence analysis revealed a single open reading frame comprising 1503 base pairs (Fig. 1, positions 130 –1632) corresponding to a polypeptide of 501 amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial a ...
... GAPDH from T. tenax—The sequence analysis revealed a single open reading frame comprising 1503 base pairs (Fig. 1, positions 130 –1632) corresponding to a polypeptide of 501 amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial a ...
Farnesyl diphosphate synthase, the target for nitrogen
... are cytosolic [10]. Hence, at present, the intracellular location of the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are peroxisomal, and that there may also be mitochondrial isoenzymes o ...
... are cytosolic [10]. Hence, at present, the intracellular location of the mammalian mevalonate pathway enzymes remains unclear. The pathway has also been investigated in plants and it appears that some, but not all, of the enzymes are peroxisomal, and that there may also be mitochondrial isoenzymes o ...
The Role of Carbohydrate Response Element Binding Protein in
... of obesity and related diseases [1–5]. Increased fructose consumption contributes to the development of obesity accompanied by glucose intolerance, fatty liver, dyslipidemia, and hyperuricemia [3]. Additionally, in experimental animals, excess fructose intake causes body weight gain and fatty liver ...
... of obesity and related diseases [1–5]. Increased fructose consumption contributes to the development of obesity accompanied by glucose intolerance, fatty liver, dyslipidemia, and hyperuricemia [3]. Additionally, in experimental animals, excess fructose intake causes body weight gain and fatty liver ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hours at 4°C followed by incubation with a 1:4000 dilution ...
... The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hours at 4°C followed by incubation with a 1:4000 dilution ...