pdf file - College of Computer, Mathematical, and Natural Sciences
... Restriction fragments for mutant allele ...
... Restriction fragments for mutant allele ...
Genome segment 5 of Antheraea mylitta cytoplasmic polyhedrosis
... mutagenesis at K21A in KLRS motif, and H93A or H105A in HxnH motif completely abolished the autoguanylylation activity and indicates importance of these residues at these sites. Thermodynamic analysis showed p65-GTP interaction was primarily driven by enthalpy (ΔH = −399.1 ± 4.1 kJ/mol) whereas the ...
... mutagenesis at K21A in KLRS motif, and H93A or H105A in HxnH motif completely abolished the autoguanylylation activity and indicates importance of these residues at these sites. Thermodynamic analysis showed p65-GTP interaction was primarily driven by enthalpy (ΔH = −399.1 ± 4.1 kJ/mol) whereas the ...
Disallowed Ramachandran Conformations of Amino Acid Residues
... An analysis of the nature and distribution of disallowed Ramachandran conformations of amino acid residues observed in high resolution protein crystal structures has been carried out. A data set consisting of 110 high resolution, non-homologous, protein crystal structures from the Brookhaven Protein ...
... An analysis of the nature and distribution of disallowed Ramachandran conformations of amino acid residues observed in high resolution protein crystal structures has been carried out. A data set consisting of 110 high resolution, non-homologous, protein crystal structures from the Brookhaven Protein ...
Refining the Definition of Plant Mitochondrial
... analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., 2001; Zhang and Glaser, 2002). However, this data set ...
... analysis of 58 experimentally proven plant mitochondrial presequences deposited in the Swiss-Prot database revealed two major classes containing an Arg residue at positions 22 and 23 and one class without any conserved Arg residues (Zhang et al., 2001; Zhang and Glaser, 2002). However, this data set ...
Gene Section SOX10 (SRY (sex determining region Y)-box 10)
... family possess a high-mobility-group (HMG) domain with three alpha-helical regions and close similarity to the one found in the male sex determining factor SRY. SOX10 functions as transcription factor and structural protein in chromatin. SOX9 and SOX8 are its closest relatives among human SOX protei ...
... family possess a high-mobility-group (HMG) domain with three alpha-helical regions and close similarity to the one found in the male sex determining factor SRY. SOX10 functions as transcription factor and structural protein in chromatin. SOX9 and SOX8 are its closest relatives among human SOX protei ...
Biochemical, biophysical and interaction studies of the stress
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
... Firstly I would like to thank all my supervisors, Dr Alexander Golovanov, Dr Costas Demonacos and Dr Stephen Prince for their continual guidance throughout this PhD. I would also like to thank my adviser Professor Andrew Doig for all his support and advice on PhD related issues and CD experiments. A ...
Products for Solid State NMR - Sigma
... While minimal media products are the basic tools to facilitate the incorporation of a uniform isotopic label for Solid-State NMR experiments, there are potential problems which may arise when relying on this method exclusively. There can be difficulties expressing sufficient quantities of certain pr ...
... While minimal media products are the basic tools to facilitate the incorporation of a uniform isotopic label for Solid-State NMR experiments, there are potential problems which may arise when relying on this method exclusively. There can be difficulties expressing sufficient quantities of certain pr ...
Amino acid residues that determine functional specificity of NADP
... them in a number of technical details, which make SDPpred procedure more reliable and fully automated: SDPpred takes into account evolutionary and physicochemical similarities of amino acids enclosed in amino acid substitution matrices; it corrects the statistics basing on real evolutionary distance ...
... them in a number of technical details, which make SDPpred procedure more reliable and fully automated: SDPpred takes into account evolutionary and physicochemical similarities of amino acids enclosed in amino acid substitution matrices; it corrects the statistics basing on real evolutionary distance ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... Compared with control cells, overexpression of CrAT caused a significant reduction in the production of some esters, including the important flavour components ethyl acetate and 3-methyl-butyl acetate (isoamyl acetate). ...
... Compared with control cells, overexpression of CrAT caused a significant reduction in the production of some esters, including the important flavour components ethyl acetate and 3-methyl-butyl acetate (isoamyl acetate). ...
Comparison of Genes Encoding Enzymes of Sterol Biosynthesis
... follows different routes in photosynthetic and nonphotosynthetic organisms. Accordingly, to open the cyclopropane ring of cycloartenol, the photosynthetic organisms require different set of enzymes, such as cycloartenol synthase and cycloeucalenol-obtusifoliol isomerase. Nevertheless, the sterol bio ...
... follows different routes in photosynthetic and nonphotosynthetic organisms. Accordingly, to open the cyclopropane ring of cycloartenol, the photosynthetic organisms require different set of enzymes, such as cycloartenol synthase and cycloeucalenol-obtusifoliol isomerase. Nevertheless, the sterol bio ...
Multiple Sequence Motifs in the Rubisco Small
... Figure 1. Protein import of RbcS-tp:GFP and E1a-tp:GFP in wild-type and ppi2 mutant protoplasts. A, Sequences of RbcS-tp and E1a-tp. TP cleavage sites were predicted by TargetP (Emanuelsson et al., 2000) and indicated by arrowheads. B, Images of RbcStp:GFP and E1a-tp:GFP. Protoplasts from wild-type ...
... Figure 1. Protein import of RbcS-tp:GFP and E1a-tp:GFP in wild-type and ppi2 mutant protoplasts. A, Sequences of RbcS-tp and E1a-tp. TP cleavage sites were predicted by TargetP (Emanuelsson et al., 2000) and indicated by arrowheads. B, Images of RbcStp:GFP and E1a-tp:GFP. Protoplasts from wild-type ...
Highly Conserved Region 141–168 of the NS1 Protein Is a New
... disease, namely DHF or dengue shock syndrome, is increasing (1,2). Dengue virus (DENV) has four serotypes (DENV 1–4), all of which are responsible for severe forms of the disease. The World Health Organization currently estimates that there are 50 to 100 million dengue infections globally each year, ...
... disease, namely DHF or dengue shock syndrome, is increasing (1,2). Dengue virus (DENV) has four serotypes (DENV 1–4), all of which are responsible for severe forms of the disease. The World Health Organization currently estimates that there are 50 to 100 million dengue infections globally each year, ...
FIST/HIPK3: a Fas/FADD-interacting Serine/Threonine Kinase that
... Figure 1. Structure, expression and enzymatic activity of FIST/HIPK3. (A and B) Predicted aa sequence and structural organization of human and murine FIST/HIPK3. FIST/HIPK3 contains a kinase domain and a PEST sequence, which are linked by a region of ⵑ200 aa that was identified to interact with the ...
... Figure 1. Structure, expression and enzymatic activity of FIST/HIPK3. (A and B) Predicted aa sequence and structural organization of human and murine FIST/HIPK3. FIST/HIPK3 contains a kinase domain and a PEST sequence, which are linked by a region of ⵑ200 aa that was identified to interact with the ...
The regulation of leukemia inhibitory factor
... The expression of LIF is also regulated by estrogen, especially for the induction of LIF in the uterine tissue at the embryonic implantation stage. The estrogen levels increase significantly at the implantation stage in both mice and humans to induce LIF mRNA levels in the uterine tissues, which is ...
... The expression of LIF is also regulated by estrogen, especially for the induction of LIF in the uterine tissue at the embryonic implantation stage. The estrogen levels increase significantly at the implantation stage in both mice and humans to induce LIF mRNA levels in the uterine tissues, which is ...
Simultaneous Alignment and Folding of Protein Sequences
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
... structure. Moreover, for proteins, there is no clear chemical basis for compensatory mutations [11], the energy models that define β-strand pairings are more complex, and the larger residue alphabet vastly increases the complexity of the problem. This class of problems is also different than any tha ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... positions. The remaining 25 vertebrate sequences were aligned, of which a sampling of the most divergent representatives is given in Fig. 1, together with 2 plant and 2 yeast sequences. The plant and yeast sequences were the most closely related nonvertebrate aldo-keto reductases in the original dat ...
... positions. The remaining 25 vertebrate sequences were aligned, of which a sampling of the most divergent representatives is given in Fig. 1, together with 2 plant and 2 yeast sequences. The plant and yeast sequences were the most closely related nonvertebrate aldo-keto reductases in the original dat ...
The different roles of tryptophan transfer RNA in regulating trp
... coding region stalls at one of its two Trp codons. Antiterminator formation prevents formation of the terminator. The third alternative RNA structure, 1:2, serves three roles. As a transcription pause structure it is essential for the coupling of transcription and translation in the leader region. A ...
... coding region stalls at one of its two Trp codons. Antiterminator formation prevents formation of the terminator. The third alternative RNA structure, 1:2, serves three roles. As a transcription pause structure it is essential for the coupling of transcription and translation in the leader region. A ...
Tangping Li, Ke Fan, Jun Wang and Wei Wang Reduction of protein
... P in each group (n1, n2, ¼, nN) (Wang and Wang, 1999) with Ni1 ni = 20, we can always ®nd a speci®c grouping of residues with a maximal value of S, i.e. S = S¢max. Such a grouping is regarded as the best or the most reasonable one since it may reserve the maximal information on the parent sequence ...
... P in each group (n1, n2, ¼, nN) (Wang and Wang, 1999) with Ni1 ni = 20, we can always ®nd a speci®c grouping of residues with a maximal value of S, i.e. S = S¢max. Such a grouping is regarded as the best or the most reasonable one since it may reserve the maximal information on the parent sequence ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... map are shown in Fig. 2) revealed a single long open reading frame of 678 nucleotides, beginning at the first ATG codon encountered in Cx26-1 (278 nucleotides from the 5' end). This has the appropriate context for a eukaryotic translational initiation site with an A three nucleotides upstream and a ...
... map are shown in Fig. 2) revealed a single long open reading frame of 678 nucleotides, beginning at the first ATG codon encountered in Cx26-1 (278 nucleotides from the 5' end). This has the appropriate context for a eukaryotic translational initiation site with an A three nucleotides upstream and a ...
Sample Chapter 5: Amino Acids, Peptides, and Proteins
... that protein. The ability of each type of the tens of thousands of different proteins to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimensional space as its amino acid components (called amino acid residues) interac ...
... that protein. The ability of each type of the tens of thousands of different proteins to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimensional space as its amino acid components (called amino acid residues) interac ...
PPT
... • FDM - Fragment Data Mining - exploits availability of sequencesimilar fragments in the PDB, which can lead to highly accurate prediction - much better than GOR V - for such fragments, but such fragments are not available for many cases ...
... • FDM - Fragment Data Mining - exploits availability of sequencesimilar fragments in the PDB, which can lead to highly accurate prediction - much better than GOR V - for such fragments, but such fragments are not available for many cases ...
Cloning, Sequencing, and Characterization of Luciola italica
... amino acids. Initial characterization of the enzyme showed that the L. italica protein exhibits bioluminescent activity similar in intensity to the common North American Photinus pyralis luciferase; however it produces light that is slightly red-shifted (having maximum emission at 564 nm). By steady ...
... amino acids. Initial characterization of the enzyme showed that the L. italica protein exhibits bioluminescent activity similar in intensity to the common North American Photinus pyralis luciferase; however it produces light that is slightly red-shifted (having maximum emission at 564 nm). By steady ...
ppt - Bio 5068
... • Can help narrow construct space and identify interfaces •regions that interact with solvent or binding partners ...
... • Can help narrow construct space and identify interfaces •regions that interact with solvent or binding partners ...
Serine/Threonine Phosphatases
... of PSPs have been documented with increasing detail. An earlier emphasis on functional characterization has recently been complemented by biochemical and structural investigations of all three major families of PSPs, giving rise to major advances in mechanistic understanding. The cellular and physio ...
... of PSPs have been documented with increasing detail. An earlier emphasis on functional characterization has recently been complemented by biochemical and structural investigations of all three major families of PSPs, giving rise to major advances in mechanistic understanding. The cellular and physio ...
A Survey of Left-handed Helices in Protein Structures
... structural parameters.5 To our knowledge, the handedness of helices in proteins has not been studied systematically, and not much is known about left-handed helices other than that they are very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appea ...
... structural parameters.5 To our knowledge, the handedness of helices in proteins has not been studied systematically, and not much is known about left-handed helices other than that they are very rare. Stretches of amino acids with unusual backbone conformations (e.g. left-handed helices) often appea ...