Protein Structure Hierarchy
... backbone, sticks, spacefill, ball & stick, ribbons, strands and cartoons) from the “pull-down” menus. Think about the advantages and disadvantages of these different representations are you see them. Choosing the best way to view a structural detail can only be learned through experience. ...
... backbone, sticks, spacefill, ball & stick, ribbons, strands and cartoons) from the “pull-down” menus. Think about the advantages and disadvantages of these different representations are you see them. Choosing the best way to view a structural detail can only be learned through experience. ...
... RECEPTOR-DELTA IN THE SKELETAL MUSCLE .............................. 41 6.1. PPARδ transcription in the skeletal muscle ................................. 43 6.2. PPARδ and fibre type conversion ................................................ 43 6.3. PPARδ, lipid metabolism and oxidation ........... ...
The semi-phosphorylative Entner–Doudoroff pathway in
... a comparative genomics approach has revealed ED gene clusters that are conserved in T. tenax [4], S. solfataricus, S. tokodaii and Halobacterium sp. NRC. We present here a qualitative analysis of the corresponding gene products of T. tenax and S. solfataricus. A detailed biochemical analysis of the ...
... a comparative genomics approach has revealed ED gene clusters that are conserved in T. tenax [4], S. solfataricus, S. tokodaii and Halobacterium sp. NRC. We present here a qualitative analysis of the corresponding gene products of T. tenax and S. solfataricus. A detailed biochemical analysis of the ...
Mammalian CSAD and GADL1 have distinct biochemical properties
... decarboxylase 67 (GAD67). CSAD levels were highest during prenatal and early postnatal development; GADL1 peaked early in prenatal development, while GAD67 increased rapidly after birth. Both CSAD and GADL1 are being expressed in neurons, whereas only CSAD mRNA was detected in astrocytes. Cysteine s ...
... decarboxylase 67 (GAD67). CSAD levels were highest during prenatal and early postnatal development; GADL1 peaked early in prenatal development, while GAD67 increased rapidly after birth. Both CSAD and GADL1 are being expressed in neurons, whereas only CSAD mRNA was detected in astrocytes. Cysteine s ...
High pKa variability of cysteine residues in structural databases and
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
Predicting DNA-binding sites of proteins from amino acid sequence
... More recently, methods for precisely identifying proteinDNA contacts by coupling photochemical crosslinking with mass spectrometry have also been developed [9]. With increasing availability of protein sequence data, there is an urgent need for computational tools that can rapidly and reliably identi ...
... More recently, methods for precisely identifying proteinDNA contacts by coupling photochemical crosslinking with mass spectrometry have also been developed [9]. With increasing availability of protein sequence data, there is an urgent need for computational tools that can rapidly and reliably identi ...
Saccharomyces cerevisiae.
... intended pathway, the effects are often only partial. Yet another possible industrial application of anaerobic growth lies in the transfer of this ability to other organisms. For example, the yeast Kluyveromyces lactis can utilize lactose as a sole carbon source. This sugar is the major component of ...
... intended pathway, the effects are often only partial. Yet another possible industrial application of anaerobic growth lies in the transfer of this ability to other organisms. For example, the yeast Kluyveromyces lactis can utilize lactose as a sole carbon source. This sugar is the major component of ...
Cells Phenotype of Human Tolerogenic Dendritic Glycolytic
... to identify energetic metabolism “signatures” in each of the moDCs. This analysis revealed a DEG pattern for tolerogenic moDCs relative to the other moDCs (Fig. 1A, left panel). IPA showed that this tolerogenic moDC-specific DEG profile was enriched for pathways involved in energy metabolism, includ ...
... to identify energetic metabolism “signatures” in each of the moDCs. This analysis revealed a DEG pattern for tolerogenic moDCs relative to the other moDCs (Fig. 1A, left panel). IPA showed that this tolerogenic moDC-specific DEG profile was enriched for pathways involved in energy metabolism, includ ...
Characterization of the cDNA and Gene Coding for the Biotin
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
... pBS-1 was confirmed upon sequencing of the corresponding gene (see below). At the 3' end, the pBS-1 sequence contains an extra 58 and 19 nucleotides upstream of the poly(A) tail that are not present in the sequence shown in GenBank accession number L34413 or in the sequence of Patton et al., respect ...
PPTX - Bonham Chemistry
... • The enzyme beta glucanase is active during this rest. This enzyme breaks down beta glucans. Under modified malts and some adjuncts with high levels of beta glucans can lead to stuck mashes. ...
... • The enzyme beta glucanase is active during this rest. This enzyme breaks down beta glucans. Under modified malts and some adjuncts with high levels of beta glucans can lead to stuck mashes. ...
An introduction to informatics - Swiss
... http://www.ebi.ac.uk/swissprot/Submissions/submissions.html UniProtKB: Questions and answers 31.VII.2006 ...
... http://www.ebi.ac.uk/swissprot/Submissions/submissions.html UniProtKB: Questions and answers 31.VII.2006 ...
Transport proteins regulate the flux of metabolites and cofactors
... metabolite and cofactor transporters is required and needs to be efficiently coordinated. These peroxisomal transport proteins are a prerequisite for metabolic reactions inside plant peroxisomes. The entire peroxisomal “permeome” is closely linked to the adaption of photosynthetic organisms during la ...
... metabolite and cofactor transporters is required and needs to be efficiently coordinated. These peroxisomal transport proteins are a prerequisite for metabolic reactions inside plant peroxisomes. The entire peroxisomal “permeome” is closely linked to the adaption of photosynthetic organisms during la ...
General principles of cellular organization in the genome
... One aim of research in natural sciences is to understand a self-reproducing cell in its entity. Transcriptome, proteome and metabolome are at the heart of cellular organization. To know the general principles underlying these basic functional aspects of a cell is a key part on the way to comprehensi ...
... One aim of research in natural sciences is to understand a self-reproducing cell in its entity. Transcriptome, proteome and metabolome are at the heart of cellular organization. To know the general principles underlying these basic functional aspects of a cell is a key part on the way to comprehensi ...
SNAREs
... dogged the role of SNAREs in the specificity of membrane fusion events has been the apparent lack of specificity among many SNARE–SNARE associations. This lack of specificity is particularly apparent in in vitro mixing experiments using bacterially expressed mammalian as well as yeast SNARE proteins (Y ...
... dogged the role of SNAREs in the specificity of membrane fusion events has been the apparent lack of specificity among many SNARE–SNARE associations. This lack of specificity is particularly apparent in in vitro mixing experiments using bacterially expressed mammalian as well as yeast SNARE proteins (Y ...
Engineering Cytosolic Acetyl-CoA Metabolism in
... lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). The MTH1-∆T allele resulted in reduced glucose uptake, which may attenuate the repression of resp ...
... lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). The MTH1-∆T allele resulted in reduced glucose uptake, which may attenuate the repression of resp ...
Rabbit genome editing with zinc finger nucleases
... sional application No. 61/228,419, ?led Jul. 24, 2009, and is a continuation in part of Us. non-provisional application Ser. No. 12/592,852, ?led Dec. 3, 2009, Which claims priority to Us. provisional 61/200,985, ?led Dec. 4, 2008 and Us. provisional application 61/205,970, ?led Jan. 26, 2009, all o ...
... sional application No. 61/228,419, ?led Jul. 24, 2009, and is a continuation in part of Us. non-provisional application Ser. No. 12/592,852, ?led Dec. 3, 2009, Which claims priority to Us. provisional 61/200,985, ?led Dec. 4, 2008 and Us. provisional application 61/205,970, ?led Jan. 26, 2009, all o ...
Scientific Opinion on Dietary Reference Values for protein
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
... Dietary Reference Values (DRVs) for protein. The Panel concludes that a Population Reference Intake (PRI) for protein can be derived for adults, infants and children, and pregnant and lactating women based on nitrogen balance studies. The Panel also considered several health outcomes that may be ass ...
Glycolytic strategy as a tradeoff between energy yield and protein cost
... enzymes and a pyruvate kinase (pyk) (Materials and Methods, SI Text, and Tables S2 and S3). Z. mobilis, for example, is not genetically capable of the EMP pathway because it has no pfk, whereas Bacillus subtilis is not capable of the ED pathway because it lacks a functional edd enzyme (9). Fig. 2 sh ...
... enzymes and a pyruvate kinase (pyk) (Materials and Methods, SI Text, and Tables S2 and S3). Z. mobilis, for example, is not genetically capable of the EMP pathway because it has no pfk, whereas Bacillus subtilis is not capable of the ED pathway because it lacks a functional edd enzyme (9). Fig. 2 sh ...
Pseudouridine at position 55 in tRNA controls the contents of other
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
Genetic Engineering for Improved Xylose Fermentation by Yeasts
... Metabolic engineering [39, 40] has been used to impart the capacity for ethanol production and xylose fermentation in E. coli [41-45], Klebsiella oxytoca [46,47], Zymomonas mobilis [48,49] and S. cerevisiae [50-53]. In general, attempts at metabolic engineering have been more successful in bacteria ...
... Metabolic engineering [39, 40] has been used to impart the capacity for ethanol production and xylose fermentation in E. coli [41-45], Klebsiella oxytoca [46,47], Zymomonas mobilis [48,49] and S. cerevisiae [50-53]. In general, attempts at metabolic engineering have been more successful in bacteria ...
Recycling of vitamin B12 and NAD+ within the Pdu
... FMN and two [4Fe-4S] clusters which are oxygen-labile. Genetic studies showed that a pduS deletion decreased the growth rate of Salmonella on 1,2-PD supporting a role in cobalamin reduction in vivo. Further SDS-PAGE and Western blot of purified Pdu MCP and following MS-MS analysis demonstrated that ...
... FMN and two [4Fe-4S] clusters which are oxygen-labile. Genetic studies showed that a pduS deletion decreased the growth rate of Salmonella on 1,2-PD supporting a role in cobalamin reduction in vivo. Further SDS-PAGE and Western blot of purified Pdu MCP and following MS-MS analysis demonstrated that ...
METABOLIC CUES AND REGULATORY PROTEINS
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
Vanadium-Binding Proteins (Vanabins)
... vanadium-associated proteins (VAPs), in the blood cells of Ascidia sydneiensis samea [9]. These vanabins were identified by a combination of anion exchange chromatography and atomic absorption spectrometry. They include at least two major proteins with apparent molecular weights of 12.5 and 15 kDa, ...
... vanadium-associated proteins (VAPs), in the blood cells of Ascidia sydneiensis samea [9]. These vanabins were identified by a combination of anion exchange chromatography and atomic absorption spectrometry. They include at least two major proteins with apparent molecular weights of 12.5 and 15 kDa, ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
Identification of Bioactive Peptide Sequences from Amaranth
... Abstract: Amaranth (Amaranthus hypochondriacus) is a pseudocereal with higher protein concentration than most cereal grains. Enzymatic hydrolysis and food processing could produce biopeptides from amaranth proteins; however, there is limited information about the bioactivity of peptides from amarant ...
... Abstract: Amaranth (Amaranthus hypochondriacus) is a pseudocereal with higher protein concentration than most cereal grains. Enzymatic hydrolysis and food processing could produce biopeptides from amaranth proteins; however, there is limited information about the bioactivity of peptides from amarant ...