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KEY - Cell Review 1. In which organelle does DNA replication take place? nucleus 2. Which organelle enables plants to make their own food using sunlight? chloroplast 3. What cell structure gave the onionskin cells a more rigid shape than the cheek cells? Cell wall 4. Where do the organelles that make proteins originate? Nucleolus (ribosomes are made here) 5. Energy conversion within an animal cell would be severely limited by the removal of which organelle? mitochondria 6. Which organelle empties when a plant wilts (shrivels)? Central vacuole 7. Name the 2 structures in an animal cell and not found in the plant cell. Lysosome, centrioles 8. What would happen if an enzyme entered a cell and destroyed the nucleus? No proteins could be made, cell could not divide, cell would eventually die when all metabolic pathways shut down 9. What would occur if an animal cell didn’t have any lysosomes? It would be unable to break down invading particles, molecules it took in by endocytosis, or old, worn-out organelles 10. What does the cell membrane consist of? Primarily phospholipid molecules arranged in a bilayer with proteins embedded in the layer; cholesterol molecules may also be present, as well as some carbohydrate and lipid pieces attached to the proteins 11. How would the cell act if it were missing each of the following organelles? (the answer “the cell will die” is not an acceptable answer.) - Mitochondria – ATP would not be made, therefore the cell would have no energy source for many vital processes - Chloroplasts – carbohydrates (glucose) could not be made so the cells would not have a source of energy for cellular respiration, which would result in no ATP and therefore no energy source for many vital processes - Nucleus - No proteins could be made, cell could not divide, cell would eventually die when all metabolic pathways shut down - Ribosomes – protein synthesis could not occur so none of the important structural or functional (enzymatic) proteins needed by the cell could be made and the key (vital) processes of the cell would shut down 12. What type of cell needs the most mitochondria? (nerve, muscle, blood, or bacteria) Why? Of these listed, muscle cells are generally viewed as the most energy consuming and therefore you would expect to see the most mitochondria in them to produce that needed energy 13. Describe the cell theory, its parts and its contributors. The cell theory states: cells are the basic units of all life all organisms are made of 1 or more cells all cells come from other cells (biogenesis) The scientists credited with contributions to this theory are Schleiden, Schwann, and Virchow. 14. Compare and contrast a plant and animal cell. Include organelles and shape. Animal and plant cells vary in shape, however, plant cells tend to have a more rigid external appearance due to the presence of the cellulose-containing cell wall surrounding the cell/plasma membrane than animal cells which only have a cell/plasma membrane surrounding its cytoplasm. Animal cells are known to contain lysosomes, while in plants this is a point of controversy. Animal cells also have centrioles in their centrosome while plant cells do not. Plant cells have the cell wall, a large central vacuole, and chloroplasts. Animal cells do not. Further, plant cells do not have cilia or flagella while these may be present in animal cells. 15. Distinguish the two types of cells. (Prokaryotic and Eukaryotic) Include examples and pictures. Prokaryotic cells include those from the Kingdom Bacteria and the Kingdom Archaea. They do not have a nucleus or any membrane bound organelles. Eukaryotic cells are all cells in the Kingdoms Protista, Fungi, Plantae, and Animalia. They all contain a nucleus and a variety of membrane bound organelles. All cells contain: cell/plasma membrane DNA Ribosomes Cytoplasm/Cytoskeleton 16. List the contributions of the following scientists and how they are related to this unit. (CELLS) Leewenhoek: invented the first “real” microscope; looked at “animicules” under the microscope Virchow: Russian scientist who contributed the biogenesis portion of the cell theory Schwann: German zoologist who contributed to the portion of the cell theory that states that all organisms are made of one or more cells Schleiden: German botanist who contributed to the portion of the cell theory that states that all organisms are made of one or more cells Hooke: looked at cork under the microscope and coined the term “cell” to describe the little boxes he saw, which reminded him of the cells in a monastery Brown: discovered the nucleus in cells 17. Differentiate between flagella and cilia. (structure and function) eukaryotic cilia and flagella are both composed of microtubules in a sheath of plasma membrane. Both are used for locomotion of the cell, or if in a tissue, of particles past the cell. They have the same diameter. However, flagella are longer than cilia, occur only one to a few per cell, and move in a wave-like or undulating motion. Cilia are shorter, generally cover the entire surface of the cell, and move like oars rowing a boat. Prokaryotes do NOT have cilia and their flagella is very different in both structure and movement form eukaryotic flagella. It will be discussed when we discuss the bacteria later in the year. Cell Transport Review 1. What is the key difference between passive and active transport? Passive transport does not require energy and substances move from high concentration of that substance to low concentration of that substance while active transport requires energy and substances move from low concentration of that substance to high concentration of that substance. 2. What happens to a cell that is placed in a/an (also describe which way water will move) a. Isotonic solution – water will move in and out of the cell at equal rates with no net change in volume/mass of the cell b. Hypotonic solution – water will move into the cell and the cell will swell and could burst if it is an animal cell; if it is a plant cell, the cell will become turgid as the cell wall puts pressure back on the water and prevents more from entering c. Hypertonic solution – water will move out of the cell and the cell will shrink; crenate if it’s an animal cell and plasmolyze if it’s a plant cell (the membrane will pull away from the cell wall) 3. Compare and contrast diffusion and osmosis. Diffusion is the movement f a substance from an area of high concentration to low concentration. Osmosis is a special type of diffusion in which WATER moves from high concentration of water to low concentration of water. If both solute (such as salt or sugar) and solvent (water) can move across a membrane, they will move in opposite directions; i.e. solvent moves from hypertonic solution to hypotonic solution while water moves from hypotonic solution to hypertonic solution 4. Describe dynamic equilibrium. Dynamic equilibrium is when molecules are entering and leaving a solution (cell) with no net change in concentration (they may not be equal, but they are entering and leaving at the same rate so there’s no apparent change occurring) 5. Compare and contrast diffusion through a selectively permeable membrane with diffusion in an open (fully permeable) system. When a semipermeable membrane is involved, each substance diffuses down its own concentration gradient IF it can pass through the membrane. If the solutes can NOT pass through the membrane, then water will move from the hypotonic solution to the hypertonic solution. In an open system, every substance will simply move down its own concentration gradient until all substances are equally dispersed. 6. Compare and contrast diffusion through a selectively permeable membrane with facilitated diffusion. Diffusion through a selectively permeable membrane and facilitated diffusion both occur down the concentration gradient and neither requires energy (both forms of passive transport), but facilitated diffusion involves a membrane protein to move the particle across the membrane while no proteins are involved in simple diffusion. 7. Compare and contrast facilitated diffusion with active transport involving membrane proteins. Both facilitated diffusion and active transport involve membrane proteins, but facilitated diffusion is passive and requires no energy and occurs down the concentration gradient (high to low) while active transport requires energy and occurs against the concentration gradient (low to high). 8. Describe the role of the following membrane proteins in moving sugar into the cell. Remember to include what is happening to ATP. a. Proton pump – a proton pump creates a gradient down which protons can flow into the cell; when flowing through a coupled transport protein, they can facilitate the movement of sugar into the cell; ATP is required to initiate the conformational change necessary for the proton to move through the pump to the other side b. Sodium-potassium pump – sodium potassium pumps can also be used to create a gradient down which sodium can flow back into the cell and involving a coupled channel protein; ATP is used again to initiate the conformational change that results in the sodium being pumped out and potassium being attracted to the pump 9. Compare and contrast: a. Active transport involving membrane proteins with endo/exocytosis – active transport can be used to move molecules specific for the membrane protein into or out of the cell, while endocytosis and exocytosis are more generalized and take larger quantities (not a single particle) of materials into or out of the cell; both are forms of active transport, requiring energy from ATP and moving particles against their concentration gradients b. Endocytosis and exocytosis – endocytosis and exocytosis are both forms of active transport – require ATP and move material against its concentration gradient – and both involve vesicles, but endocytosis moves particles into the cell and exocytosis moves particles out of the cell c. Pinocytosis and phagocytosis – pinocytosis and phagocytosis are both forms of endocytosis, but pinocytosis involves the intake of liquid (a solution) while phagocytosis involves the intake of solids (food) 10. What cell process may result in exocytosis after the product leaves the Golgi? (Identify the product in your answer). Protein synthesis (the product being a protein) may result in exocytosis if the material being produced is to be secreted from the cell Make sure you review your notes and your homework/study guide sheets to see what kind of problems you can expect to answer on the test.