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Rational Drug Design in Anticancer Gold (I) Phosphine Complexes Alexandra Nagelski Bioinorganic Presentation December 8, 2016 “Everything is poisonous, and nothing is harmless. The dose (amount) alone defines whether something isn’t poison.” – Paracelsus, 1493-1541 Overview • Background – Medicinal Inorganic Chemistry – Au – Cancer cells • 2 Classes of Anticancer Au(I) phosphines – Linear two-coordinate complexes • Auranofin – Tetrahedral bis-chelated complexes • [Au(dppe)2]Cl • Evolution of the Research • [Au(d2pypp)2]Cl Complex – Reactivity – Mechanistic insights • Looking forward: Future Research and Implications Medicinal Inorganic Chemistry • Theme of chance versus design • Key ideas in the design – Control of toxicity – Specificity, Targeting the metal to specific tissues, organs or cells where activity needed • What parts are essential for activity: M? L? M(L)n? • Prodrugs Gold • Common oxidation states: +1, +3 – Au(I) d10 ; Au(III) d8 • “Soft” metal ion • Most stable complexes contain heavier ligands – P>N, S>O • Stabilized by π-acceptor ligands • Thiolate sulfur (Cys) or selenolate selenium (Sec) • Aqua ion unknown Brief Background Cancer Cells • Thioredoxin System – Trx • Reduces disulfides in proteins and peptides • Redox active site Cys-Gly-Pro-Cys – TrxR • Electron donor for Trx – NADPH **Elevated mitochondria membrane potential (∆Ψm) Advantage of Au • Not DNA-dependent • Works for cisplatin-resistant cancer cells Karlenius et al. Cancers (Basel). 2010, 2(2):209-232. Rackham et al. Biochem. Pharma. 2007, 74: 992-1002. Linear 2-Coordinate (Class I) Auranofin • Rheumatoid arthritis drug • Found to have anticancer properties • Forms stable adduct through displacement of the thiolate • Too reactive in vivo *Linear two-coordination Au(I): reactivity towards thiols and selenols Chelated diphosphines (Class II) • Delocalized lipophilic cations • Nernst Equation: Eion= RT/zF * ln[ion]out/ln[ion]in • Antitumour activity may stem from the lipophilic, cationic properties • Does not undergo ligand exchange as readily as linear 2-coordinate Au(I) complexes TOXIC Partition coefficient (P) Lighter octanol liquid • Log P - Used as an indicator of lipophilicity Heavier water liquid Significance: • Lipophilicity determines degree of protein binding and cellular uptake • More lipophilic, more potent, less specific Slide figures courtesy of Dr. Mark Hilfiker (Topics in Bioorganic Chemistry) Pyridylphosphine Analogs • Position of N-atom influences solvent [Au(d2pype)2]Cl interaction • Intermediate lipophilicity • 3-pyridyl, 4-pyridyl • Significant antitumor more activity soluble in H2O • Increase in potency • Less dose limiting toxicity (decrease in • Higher Au concentration in plasma selectivity) with and tumors increasing lipophilicity Log P = 1.41 Log P = -0.46 (n=2) Log P = -1.46 (n=3) Log P = -1.77 (n=4) [Au(d2pypp)2]Cl R = 2-pyridyl Log P = -0.92 Lop P = -0.46 • Analog of (d2pype) ligand, 3carbon bridge • Combines 2 classes of anticancer Au(I) phosphine complexes • Linear: more facile ligand exchange reactions with thiols/selenols • Tetrahedral: DLC properties lead to accumulation in the mitochondria Bite Angle ~84.5˚ ~96.4˚ Affandi et al. Dalt. Trans. 1997, 1411-1420. • Leads to increased reactivity due to increased chelate ring size (L exchange reactions) Biological Biological Activity Ac vity Breast cancer cancer cells cells Breast PP 22 PP 22 Au Au Cl Cl PP PP 22 22 [Au(dppe)22]Cl ]Cl [Au(dppe) Log P = 1.41 Cite this this figure figure Cite R P R P 2 2 P R P R 2 2 A u A u R P R P 2 2 Normal cells cells Normal R= C C ll R= P R P R 2 2 N N R = R = 2 p y r d y P h 2 p y r ii d y ll P h [ A u ( d 2 p y p p ) ] C l [ A u ( d 2 p y p p ) ] C l 2 2 R = 2 p y r i d y R = 2 p y r i d ll Ny N N N Lop P = -0.46 Rackham et al. Biochem. Pharma. 2007, 74: 992-1002. Mechanism • [Au(d2pypp)2]+ selectively potent to breast cancer cells • Leads to programmed cell death mediated via mitochondria • Problematic nature of TrxR enzyme – Complex structure – Catalytic mechanism unknown Rackham et al. Biochem. Pharma. 2007, 74: 992-1002. Future Research & Implications • Trx/TrxR – Mechanism – Structure – Luminescence techniques • Gold (I) Complexes – Au(PPh3)(alkynyl) – Au(I) NHC – Nano-carriers • Techniques – High resolution mass spectroscopy • Other metals? References • • • • • • • • • • • • • • • • • • • • Humphreys, A. S.; Filipovska, A.; Berners-Price, S. J.; Koutsantonis, G. A.; Skelton, B. W.; White, A. H. “Gold(I) chloride adducts of 1,3-bis(di-2pyridylphosphino)propane: synthesis, structural studies and antitumour activity” Dalt Trans. 2007, 4943-4950. P F Smith, G D Hoke, D W Alberts, P J Bugelski, S Lupo, C K Mirabelli and G F Rush. “Mechanism of toxicity of an experimental bidentate phosphine gold complexed antineoplastic agent in isolated rat hepatocytes.” Journal of Pharmacology and Experimental Therapeutics. 1989, 249(3): 944-950. S Alvarez. “Distortion Pathways of Transition Metal Coordination Polyhedra Induced by Chelating Topology” Chem. Reviews. 2015, 115: 13447-13483. 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Ligand Design in Medicinal Inorganic Chemistry. Wiley 2004, 1: 230-259. Rigobello MP, Folda A, Scutari G, Bindoli A. “The modulation of thiol redox state affects the production and metabolism of hydrogen peroxide by heart mitochondria.” Arch. Biochem. Biophys. 2005, 441(2):112-22. Rigobello MP, Folda A, Baldoin MC, Scutari G, Bindoli A. “Effect of auranofin on the mitochondrial generation of hydrogen peroxide. Role of thioredoxin reductase.” Free Radic. Res. 2005, 39(7): 687-95. Xiaodong C, Huanjiao JZ, Qunhua H, Lin L, Wang M. “Novel action and mechanism of auranofin in inhibition of vascular endothelial growth factor receptor-3dependent lymphangiogenesis” Anticancer Agents Med. Chem. 2016, 14(7): 946-954. Roder C, Thomson MJ. “Auranofin: Repurposing an Old Drug for a Golden New Age” Drugs R. D. 2015, 15(1):13-20. 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McKeage MJ, Berners-Price SJ, Galettis P, Bowen RJ, Brouwer W, Ding L, Zhuang L, Baguley BC. “Role of lipophilicity in determining cellular uptake and antitumour activity of gold phosphine complexes” Cancer Chemo and Pharma. 2000, 46(5): 343-350. Liu JJ, Galettis P, Farr A, Maharaj L, Samarasinha H, McGechan AC, Baguley BC, Bowen RJ,. Berners-Price SJ, McKeage MJ. “In vitro antitumour and hepatotoxicity profiles of Au(I) and Ag(I) bidentate pyridyl phosphine complexes and relationships to cellular uptake” Journal of Inorganic Biochemistry. 2008, 102(2): 303-310