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LESSON TITLE: Mendelian Heredity SUBJECT AREA: CP Biology GRADE LEVEL: 9th grade TIME ALLOCATION: 1 day (42 minute class) OBJECTIVES: Students will understand that a unit of heredity is called a gene and genes occur in different forms called alleles. They will know what a homozygous and heterozygous genotype is and what the phenotype will display based on its genotype. They will understand how to write genotypic and phenotypic ratios. STANDARDS: Life Science BENCHMARK C: Explain the genetic mechanisms and molecular basis of inheritance. INDICATOR 6: Explain that a unit of hereditary information is called a gene, and genes may occur in different forms called alleles (e.g., gene for pea plant height has two alleles, tall and short). GROUPING OF STUDENTS: 1. Individual work 2. Group work MATERIALS: Students: Journal, Pencil, Paper, Prepared bags of allele cards, worksheets for charting allele frequencies Teacher: Plastic sealable bags, scissors, chalk and chalkboard, worksheets for allele frequencies PRIOR KNOWLEDGE NEEDED: Students will know what a chromosome is and where genes are located. They understand that DNA carries genetic information and genetic information is passed from one generation to the next. They know the process of mitosis and meiosis. MODIFICATIONS FOR SPECIAL NEEDS: Students who are having difficulty with the inheritance of alleles can repeat the activity with other traits. They can also repeat the activity by changing the parents’ genotypes and predicting the outcomes. Students will practice Punnet Squares more. Learning Disabled: An outline for class notes for mini lecture will be supplied Students who show advanced learning and are quick to pick up the concepts can practice using more alleles for larger crosses, for example a dihybrid cross. Students can use on-line resources to research genetic abnormalities. Students can look at Mendel’s research involving P, F1, F2 generations. Students can use virtual labs exploring various genetic crosses. INSTRUCTIONAL MODEL(S) 1. Individual Work 2. Class discussion 3. Mini lecture 4. Cooperative learning-group work 5. Class Discussion PROCEDURE AND ACTIVITIES TIME ALLOCATIONS 1. Student engagement: Ask students to think about their physical features. Why do they look the way they do? Students contribute their ideas to the class. 1-2 minutes 2. Ask students to write down the different types of features that they have and where they may have gotten their features from. Encourage them to think of their parents or grandparents features and relate them to themselves. Are they the same, different, maybe a mix between the two? 2 minutes 3. Class shares ideas of specific physical features. 3 minutes 4. Mini lecture A. Characteristics/traits transferred by way of genes B. Genes are pieces of DNA that carry genetic information from one generation to the next. C. Parent carries 2 copies of an allele (any one of the alternative forms of a gene that can affect the phenotype of an organism- ex. hair color, height, eye color, etc) of a gene for each characteristic and is received by the offspring during reproduction. Individuals receive one allele from each parent. D. Allele represented by an uppercase letter and a lowercase letter. Lowercase letter is recessive and uppercase letter is dominant– give example (earlobe attachment). E. Explain what homozygous and heterozygous mean and relate it to recessiveness or dominance. F. Explain difference between genotype (the set of alleles an individual has) and phenotype (the physical appearance of the expressed alleles). G. Provide examples and have students identify the genotype and phenotype. H. Introduce Mendel’s experiments on peas and his findings about genotypic and phenotypic ratios. 5. Activity involving inheritance of traits using a monohybrid cross of two heterozygous parents for earlobe attachment. A. Each student receives a bag with 50 E’s and 50 e’s in it. B. Students partnered and randomly without looking pull a letter from their bag, modeling the cross between these two parents. C. Students tally mark each cross on a piece of paper, return the letters to a discard pile and repeat 49 more times. 15 minutes 15 minutes D. After completion of cross students will determine the genotypic ratios and phenotypic ratios among offspring and record this on their worksheet given to them. *see attached worksheet (Should be 1:2:1 genotypic ratio and 3:1 phenotypic ratio) 6. After all students complete activity each group will contribute their results to the class by writing their totals for each genotype possibility on the chalkboard. 7. Students will record the class data on their worksheet and calculate the genotypic and phenotypic ratios and record them on their worksheet. (Should be 1:2:1 genotypic ratio and 3:1 phenotypic ratio) Are these ratios consistent with what Mendel found? ASSESSMENT Students will complete handout (in class or at home) *see attached Last 5 minutes (if no time left students can write their findings on board the following day) Name ______________________ Date _______________ Partner _________________ Tables for Activity Table 1. Genotypes of offspring. EE Ee ee Table 2. Offspring Ratios for Individual Teams Genotypes: Total (count tallies) Genotypic Ratio Homozygous dominant (EE) Heterozygous (Ee) Homozygous recessive (ee) _______ : ______ : _______ Phenotypes: Phenotypic Ratio Unattached earlobes (EE and Ee) Attached earlobes (ee) _______ : _______ Table 3. Offspring Ratios for Entire Class Genotypes: Total (count for class) Genotypic Ratio Homozygous dominant (EE) Heterozygous (Ee) Homozygous recessive (ee) Phenotypes: _______ : _______ : _______ Phenotypic Ratio Unattached earlobes (EE and Ee) Attached earlobes (ee) __________ : __________ Assignment Name ___________________________ Period Date ___________ 1. What are the genotypes and the phenotypes of the parents? 2. What does each letter in the bags represent? 3. Describe the genotypes of the parents and offspring using the vocabulary homozygous and heterozygous. 4. Did table 2 reflect a classic monohybrid cross phenotypic ratio of 3:1 ? If not, what could be the reason why? 5. What trait is being studied in the activity? BONUS: Construct a Punnet Square showing the parents and their offspring from the activity in class. (You may have to refer to your book) References Ohio Department of Education. (2007). Teaching, Learning, Lessons, and Units. Retrieved October 20, 2007, from http://ode.state.oh.us/IMS