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Diagnostic Test Scientific Investigation 1. A biology class wanted to develop a research project to predict the effects of a new highway on wildflower species found in the Piedmont region of Virginia. The class could best conduct such a study by sampling flowers found in the highway construction area — A. one year before highway construction begins B. both before and after highway construction is completed _ C. immediately after highway construction is finished D. during the time highway construction is taking place 2. People long ago believed that maggots came from meat. In the late 1600s, Francesco Redi made the hypothesis that maggots came from flies rather than from meat. Which of these experimental designs could be used to test Redi’s hypothesis? 3. According to the table, which graph below illustrates the calories used for 1 hour of jogging followed by 2 hours of walking? 4. Which sentence best states the importance of using control groups? A Control groups allow comparison between subjects receiving a treatment and those receiving no treatment. _ B Control groups eliminate the need for large sample sizes, reducing the number of measurements needed. C Control groups eliminate the need for statistical tests and simplify calculations. D Control groups provide a method by which statistical variability can be reduced 5. The hypothesis best supported by this graph is that these beetles are most active when the area is — A free from predators B coolest with some sunlight _ C wettest from dew D richest in oxygen supplies 6. In 1928, the scientist Alexander Fleming was trying to grow the bacterium Staphylococcus, and by chance, discovered that it would not grow in the presence of a mold that he named Penicillium. In 1939, Ernst Chain and Howard Florey purified penicillin, and in 1941, penicillin was first used to treat bacterial diseases. In 1945, Alexander Fleming, Ernst Chain, and Howard Florey received the Nobel Prize in medicine for the discovery and isolation of penicillin. These facts show that — A Florey and Chain unfairly used Fleming’s work and should not have received the Nobel Prize B chance plays no role in scientific discoveries C scientific progress often requires the efforts of many people working together_ D whenever possible, the Nobel Prize should be given to more than one person 7. Which of these hypotheses is best supported by observations of this animal? A This animal spends much of its time digging. _ B This animal usually hunts for food at night. C This animal is herbivorous. D This animal has poor hearing. 8. The data show the growth of two bean plants over several weeks using four different fertilizers. The experimental data would be more valid if which of the following variables was included in the experiment? A A fifth fertilizer was tested. B Only one plant was tested. C The plants were grown at variable temperatures. D A control without fertilizer was included for each plant. _ 9. The most valid and reliable test of this hypothesis would include an experimental group of pine seeds that was recovered from a fire area and pine seeds that were — A germinated after a fire B tolerant of fire C found before a fire _ D placed in a fire 10. A student wanted to study the effect of temperature on algae levels in a local stream. Which items are most useful for gathering data and most appropriate for communicating the results of her observations? A Research on the Internet and videotapes of water samples B Microscopes and written descriptions of weather patterns C pH strips and written observations of stream water D Test tubes, thermometers, and graphs of results 11. Jan consistently read the volume of liquids as shown. How would this practice impact her work? A Her measurements would lack precision. B Her measurements would be too high. C Her measurements would be too low for less dense liquids. D Her measurements would be very accurate. 12. Which hypothesis is best supported by this graph? A A disease of cardinals spread throughout the park. B Dominant cardinal chicks were the first to be fed. C The population of cardinal predators increased. D The cardinals’ food supply increased. 13. Scientists have noted a decline in fungi species dating back to the early 1900s. Annual crops of edible mushrooms in France and Germany have declined since 1950. One hypothesis to explain this decline is that edible fungi are being overharvested. Global warming and air pollution have also been considered as contributing to the decline. Which of the following would exclude overharvesting as the cause of the decline? A Fungal-population increases in tropical regions B Similar declines among edible North American species C High fungal numbers in nitrogen-poor soils D A parallel decline in non-edible species 14. Which factor would need to be known before a valid conclusion could be based upon these data? A The original average height of grass _ B The length of the study period C The density of the phosphate solutions D The mineral content of the potting soil 15. A student wanted to look at plant growth in five different soil samples. He planted the same type of seeds in identical containers and left them together in full sunlight. He gave each plant the same amount of water and charted the growth of each plant stem. What is the independent variable in this experiment? A Soil _ B Light C Container D Seeds 16. In the table, which item of data is most likely to be invalid? A Plot 1 on July 1 B Plot 2 on June 15 _ C Plot 3 on May 15 D Plot 4 on May 30 17. In the lab setup pictured, a student is trying to determine the effect of pollutants on the growth of three groups of seeds. The results will not be valid because the experiment has — A no conclusion B no hypothesis C too few experimental groups D too many variables 18. Orchids were studied to determine if the amount of humidity affected the flowering of these plants. Which of these was the independent variable in this study? A The percentage of humidity _ B The amount watered C The length of time required for flowering D The number of flowers on each plant 19. Richard was observing black swallowtail butterflies in the field. Which one of these is an observation he made about a black swallowtail butterfly? A The black swallowtail is closely related to the spicebush swallowtail. B The black swallowtail belongs to the class Insecta. C The black swallowtail likes to fly more than anything else. D The black swallowtail laid its eggs on a parsley plant. 20. Which variable appears to control leaf production in these plants? A The amount of water B The temperature _ C The number of daylight hours D The relative humidity 21. A biology class in Virginia conducted a survey of the plant species found on their school grounds. They found several plants that they didn’t recognize. What resources would be most helpful to the class in identifying the plants and determining if they were introduced as exotic species? A Biology textbooks and the encyclopedia B Virginia native plant checklists and plant identification keys _ C Fossil records and historical society publications D Virginia newspapers and science journals 22. A student hypothesizes that thick leg muscles are an inherited trait in dogs. The student collects data on several dogs, and the data show that dogs that live outdoors have thicker leg muscles than dogs that live indoors. What should the student conclude? A Dogs that inherit thick leg muscles may not survive indoors. B Dogs with thick leg muscles may require more exercise than dogs with thin leg muscles. C Inheritance alone may not account for thick leg muscles in dogs. _ D Inheritance of thick leg muscles may be associated with coat thickness in dogs. 23. A company that produces Brand X flea shampoo claims to have the most effective shampoo for killing fleas. Which of these sets of data supports the Brand X claim? 24. Francesco Redi performed an experiment in 1668. In the experiment, he placed rotting meat in two jars. The first jar was left open. After a few days, fly larvae were found on the decaying meat inside. The mouth of the second jar was covered with gauze. After a few days, the decaying meat inside was free of fly larvae, but larvae were found on the gauze. This experiment supports the hypothesis that — A adult flies are not attracted to decaying meat B fly larvae prefer fresh meat C fly larvae only come from adult flies _ D decaying meat produces fly larvae 25. Harvester ants often strip a bush of all of its leaves. Some people believe this helps the plant grow thicker, healthier stems. In an experiment, a student stripped off all the leaves from a set of plants. In a second set of identical plants, the student allowed ants to strip off the plants’ leaves. In order to improve this experimental design, it is most important to add a set of plants — A exposed to a different ant species B of a different species C with its leaves left intact _ D that are dormant 26. These data were collected by observing responses of different organisms to light. Which conclusion is supported by these data? A Organisms that use photosynthesis are attracted to light. _ B Protists are not attracted to light. C Animals are attracted to light. D Decomposers are attracted to light 27. Which question cannot be answered scientifically because the quantity cannot actually be measured? A How fast can a tiger swallowtail butterfly fly? B How heavy is a mature female elephant? C How happy is a chimpanzee when it finds its favorite food? _ D How much food does a water buffalo consume in one day? 28. In 1869, DNA was discovered within the nuclei of cells. By the 1940s, scientists knew that chromosomes were made of both DNA and protein but did not know which was the genetic material of cells. In the 1950s, scientists demonstrated that DNA is the material responsible for heredity. In 1953, using information collected by other scientists, an American biologist and an English physicist built a three-dimensional model of DNA. These discoveries best illustrate the importance of — A independent research B replication of results C collaborative efforts among scientists _ D recent improvements in the scientific method 29. Four disinfectants were tested in two trials, each for their effectiveness in controlling bacterial growth. The table shows the bacterial growth in each trial after four days. Which of the following conclusions is best supported by the results of this study? A Disinfectants kill most bacteria on contact. B Strong concentrations of disinfectants can be harmful. C Some disinfectants are more effective than others. _ D Disinfectants cannot be used to control bacterial infections 30. Which data point on the graph is probably invalid? A1 B2 C3 D4 31. In an experiment, the height of several plants was recorded daily in millimeters. Which tool would be the most accurate and appropriate for this measurement? A Digital scale B Graduated cylinder C Yardstick D Metric ruler 32. The results of Pasteur’s experiment helped Pasteur to — A reject the theory of spontaneous generation B isolate the virus responsible for smallpox C produce a vaccine against rabies D convince people to cover food 33. A scientist designed an experiment to test the effect of temperature on bacterial growth. He grew three different cultures of the bacterium E. coli under three heat lamps at different temperatures. What was the independent variable in this experiment? A Length of the experiment B Number of bacteria C Reproduction rate D Temperature 34. A student soaked 50 lima bean seeds and 50 green bean seeds in water for24 hours. Then she planted the seeds in soil at a depth of two centimeters, using the same amount of water, light, and heat for each kind of seed. She measured every three days to determine which type of seed grew the fastest. The paragraph describes — A an observation C a hypothesis B an experiment D a conclusion 35. Which of these would be the best conclusion regarding the experimental results shown? A The tallest this species of plant will ever grow is 50 cm. B This species of plant grew best between 18°C and 26°C. C The ideal temperature for all plant growth is 26°C. D Plants will not grow above 26°C. 36. Biologists surveyed four islands in a chain near a continent. They identified species found on the mainland and those species that were unique to each island. Based on these results, which island is probably farthest from the mainland? A1 B2 C3 D4 37. In a student experiment, 4 earthworms were placed at each end of a compartment as shown. After 10 minutes, all of the earthworms were together in the same area. The students concluded that the earthworms had moved as far as they could in that period of time. Which alternative explanation is also supported? A The earthworms moved to their preferred temperature range. B The earthworms needed to be with other earthworms for warmth. C The earthworms preferred to be in protected corners. D The earthworms moved randomly until they grouped together. 38. A scientist observes that a species of insect appears to be more numerous during dry summers than during wet summers. Which is the next step to determine an explanation of this phenomenon? A Develop a hypothesis B Publish the observation C Observe the species in the winter D Present a theory in a scientific journal 39. A student plans to conduct an experiment to determine how a change in the pH of water affects the population growth rate of algae. To ensure that her experimental data are valid, she should — A hold the acidity of the water constant B vary the temperature of the water used C control the final algae population sizes D use the same species of algae in all trials 40. 41. A student constructs several terrariums like the one shown. Each terrarium is exposed to a different amount of sunlight each day. In order to determine the ideal amount of sunlight, which of the following variables must be held constant? A Type of plants B Growth rate of plants C Wavelengths of sunlight D Amount of sunlight received As part of an experiment to measure decomposition rates of different materials, students put food scraps from the cafeteria in compost bin A and leaves and grass clippings in compost bin B for six weeks. Students in first period measured the temperature in bin A, and students in sixth period measured the temperature in bin B. What is the greatest error in the students’ experimental design? A There are too many uncontrolled variables in the experiment. B Temperature is the only dependent variable in the experiment. C The materials chosen decompose too rapidly. D The students put equal masses of materials in each bin. 42. A student hypothesizes that green algae will grow fastest when exposed to blue light. To test this hypothesis, the student should design an experiment with which independent variable? A Color of algae B Rate of algae growth C Color of light that algae are exposed to D Amount of time per day that algae are exposed to light 43. The diagram shows a setup for a plant investigation. Which variable is most likely being tested? A Hours of light exposure B Plant species C Soil volume D Soil pH 44. Students in a biology class thought that salt water would affect the movements of planaria. They put 25 planaria in salt water and recorded their observations. Which flaw was present in the design of their experiment? A There was no hypothesis. B There was no control. C The sample was too big. D The procedure was too complicated. 45. A scientist develops a hypothesis, designs an experiment, and obtains data that support her hypothesis. Which of the following best describes when a hypothesis becomes a theory? A When one good set of scientific data supports a theory B When the official scientific method is followed C When a website is created to display the theory D When it is supported by consistent data from many experimental trials