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Anatomy & Physiology 34B Lab Exam #2 Study Recommendations Lab exam #2 will consist of questions to assess your understanding of information from Labs 29 A & B (blood), 30 (heart), 31 (electrocardiography), 32 (blood vessels), 33A (blood pressure & pulse), 33B (cardiovascular dynamics), Lab 34B (frog cardiology), and 35A (lymphatics). The exam will be mainly fill-ins and short answer questions. Be sure to refer to the lab manual explanations in each lab and the Review Sheets assigned for homework as you prepare for the exam. A review of the notes that pertain to these exercises would be helpful. Also, be able to apply the information learned to real life situations. Labs 29A & B – Blood - What is blood composed of? What does plasma contain? - Be able to identify the different types of blood cells under the microscope, the function of each type, and their relative percentages in whole blood. - What is the main function of RBCs? What protein allows them to perform their function? - How are basic blood typing tests conducted? Be able to interpret blood type test results. - What is measured in a hematocrit test? What are normal hematocrit levels for men and women? What conditions are indicated by too high or too low hematocrit levels? - How is a differential WBC count conducted? What are the normal percentages for the different types of WBCs? What can be determined if a particular WBC type is not within normal range? - What are normal hemoglobin levels in men and women? What do abnormal levels indicate? - What is considered to be a healthy cholesterol level? Why types of problems can occur with high or low cholesterol levels? Lab 30 – Heart Structure - Be able to identify the structures of the heart listed on the lab handout on heart models and on a sheep heart, if applicable. - Be able to describe the sequence of blood flow through the heart. - Be able to identify the nodal tissues on a heart model, and explain the sequence of electrical conduction through the nodal tissues, and their effects on heart function. Lab 31 – Electrocardiography - Describe the components of the electrical conduction system of the heart. - Be able to label the major waves, segments, and intervals of an electrocardiogram and explain what each of these indicates in terms of the electrical conduction system of the heart. - Be able to calculate heart rate from an ECG. - In what ways did the ECG change during lying down, sitting, and exercising? - Be able to interpret an abnormal ECG. (It would be helpful to review the abnormal ECGs shown in the textbook chapter on the heart.) - What is Einthoven’s triangle? Why were 3 leads used to obtain the ECG? - Explain the different types of abnormal rhythms of the heart. Lab 32 – Blood Vessel Anatomy - Be able to identify the blood vessels listed on the lab handout on blood vessel models and in dissected cats, if applicable. - Know where the blood vessels are taking blood to and from in the body. Lab 33A – Blood Pressure & Pulse Determinations - What are the ausculatory areas for each of the 4 heart valves? - Where are the main body regions for palpating superficial pulse points? What is an average pulse rate? - What is pulse pressure, and how is it calculated? What is a normal pulse pressure? What does a high or low pulse pressure indicate? - What two instruments did we use to measure blood pressure? What is considered a normal BP range? What can occur if someone has BP lower or higher than normal? - What are the two main factors that affect blood pressure? - How did sitting, reclining, standing, and exercising affect the subject’s pulse and blood pressure? - When measuring blood pressure, how did you distinguish the systolic and diastolic pressures? - What are the sounds of Korotkoff? - From what artery is blood pressure measured? - What causes the systolic and diastolic blood pressures? - What is Mean Arterial Pressure, and how is it calculated? - As per the Harvard Step Test activity, be able to calculate a person’s index of physical fitness when given the person’s duration of exercise in seconds and the sum of his/her three pulse counts during recovery. Lab 33B – Cardiovascular Dynamics - Describe how pressure difference, blood vessel radius, blood viscosity, and blood vessel length affect blood flow and blood pressure. - Explain how the cardiovascular system compensates for changes in the above factors. - Explain how heart stroke volume and heart rate affect cardiac output. [CO = SV x HR] - Calculate heart stroke volume, given an end diastolic volume and end systolic volume. Lab 34B – Frog Cardiology - How does vagus nerve stimulation affect the frog’s heart rate? Under what conditions does vagus nerve stimulation occur in human bodies? - What is Ringer’s solution, and why was it placed on the frog heart between each new solution? - How does pilocarpine affect frog heart rate? - How does atropine affect frog heart rate? What is atropine’s mechanism of action? - How does epinephrine affect frog heart rate? Under what conditions would epinephrine be released in a human body? - How does digitalis affect frog heart rate? How does digitalis work? - How did increasing the external concentrations of calcium, sodium, and potassium affect frog heart rate? Explain their effects on the heart. Lab 35A - Be able to identify the lymphatic organs visible on a torso model, half head, and intestine models. (i.e., spleen, lymph nodes, tonsils, Peyer’s patches)