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Microbe Mission Answer Sheet Science Olympiad Station 1: Microbe Mission Name the type of microscope found at this station________compound light microscope____+1 Give the names of the parts and functions of each of the labeled parts on the microscopes. Numbered stickers need to go on each of these microscope parts. 2 microscopes should be placed at station 1. Parts +5 1.___Diaphragm 2.___Objective lens 3. ___Eyepiece 4.___stage clips 5.____revolving nosepiece 6.___Light source 7.__base 8.__arm 9.__course adjustment know 10__fine adjustment knob Function +5 ________control the amount of light hitting the specimen_ _________ additional magnification- from 4, 10, 40X__________ ______________ 10X magnification lens___________ ______ holds specimen or slid in place_______________ allows you to change from one objective or magnification to the next _______must have light to see object One hand should be under this part when the microscope is carried._ One hand should grasp this part when carrying the microscope for big changes in focus- only used at lowest obj power for small changes in focus used at higher obj Station 2: Microbe Mission This station will not need any supplies. Name this organelle: Mitochondria +1 Name and describe the process that occurs in this organelle: +2 Cellular respiration- taking food and breaking down to get energy What is the name of the hypothesis described to the left? Endosymbiosis or Endosymbiont or they can earn ½ pt for describe the engulfing of on prok/bacteria by another +1 What type of cell could have resulted from this process? Eukaryotic (or they can name a single celled euk- that is specific +1 Tie Breaker Question: Write the balanced equation for the process that occurs in this organelle: +1 6CO2 + 6H2O C6H12O6 +6O2 Name this organelle: Chloroplast+1 Name and describe the process that occurs in this organelle: Photosynthesis +1 converting solar E into chemical E +1 or Using light energy to make glucose Station 3: Microbe Mission Place these terms in the correct box according to their size: Antimony, Tobacco Mosiac Virus, E. coli, Hemoglobin, White Blood Cell +5 1-eukaryotes (white blood cell) 2-Prokaryotes (E.coli) 3-Viruses (tobacco mosaic virus) 4-Proteins (hemoglobin) 5-Small Molecules, Atoms (Antimony) In the time remaining at this station: List as many similarities and differences between a Prokaryotes & Eukaryotes Up to +4 Similarities: cell membrane, cytoplasm, ribosomes present, nucleic acid present, undergo cell division Differences: nucleus, membrane bound organelles, Typically 0.2-2.0 m m in diameter (prok), Typically 10-100 m m in diameter (euk), 70s ribosomes (prok) vs. 80S/70S (euk), circular DNA (prok) vs. linear DNA, binary fission (prok) vs. mitosis (euk); meiosis (euk) vs. no meiosis (prok) There are more than these- so check on any answers that you are uncertain about-on the computer Station 4: Microbe Mission Each food and/or drink item should have a label marked A-E (need two of each at the table- so that teams do not need to share or wait on another group); You will need two microscopes with prepared slides of Amoeba at this station also. For each item on the table- identify what type of microbe created or helped in the growth of each of the food product(s): +5 A:_________yeast______________(bread) B: _________bacteria__________(yogurt) C:-_________bacteria ________(green bean) D:_________yeast_______(alcohol) E:_________fungi________(penicillin, ampicillin or some type of cillin) Looking through the microscope- focus on the highest power. Sketch the microorganism you observe below. What is the magnification level o f your sketch? 400X +1 sketch +1 Name this organism: (Amoeba or Ameba for 1 pt; Protist for ½ pt) Where is this organism found in nature? They live in water, including lakes, ponds, streams, rivers, and puddles. Some can even live in the bodies of animals +1 Station 5: Microbe Mission Name the microbes that cause the diseases described below. Then explain the treatment of this disease. You can get this by breathing in air droplets from a cough or sneeze of an infected person. In the United States, most people will recover from primary infection without further evidence of the disease. The infection may stay asleep or nonactive (dormant) for years and then reactivate. Most people who develop symptoms of an infection first became infected in the past. However, in some cases, the disease may become active within weeks after the primary infection. The following people are at higher risk for active form of disease :Elderly, Infants, People with weakened immune systems, for example due to AIDS, chemotherapy, or antirejection medicines given after an organ transplant Microbe: Mycobacterium tuberculosis +1 Treatment: The standard "short" course treatment for TB is isoniazid, rifampicin (also known as rifampin in the United States), pyrazinamide, and ethambutol for two months, then isoniazid and rifampicin alone for a further four months. The patient is considered cured at six months (although there is still a relapse rate of 2 to 3%). For latent tuberculosis, the standard treatment is six to nine months of isoniazid alone. They can say “antibiotics”. +1 Microbe: Fungus +1/2 = Tinea pedis +1 The early signs of this are patches or fissures (deep breaks or slits), especially between the toes. As the infection progresses, the skin may turn red, become itchy, and appear moist. Small blisters may spread out across the foot, breaking to expose raw fissures that are painful and may swell. The area between the toes is most often affected, but the infection may spread to the soles of the feet or to the toenails, which can become thick and colored white or cloudy yellow. In the most advanced cases, the rash will extend moccasin-style across the sole of your foot, and your feet may ooze pus and develop a foul odor. Treatment: There are numerous options for treating athlete's foot. If the infection is mild (scaly white patches of skin or fissures, but no redness or itching), pay special attention to foot hygiene. Wash your feet regularly, and dry them thoroughly, especially between the toes. Apply an antifungal cream to the affected area, and dust your socks and shoes with antifungal powder. When shopping for overthe-counter remedies for athlete's foot, look for products that contain clotrimazole, econazole, ketoconazole, miconazole, naftifine, oxiconazole, sulconazole, terbinafine, or terconazole. – students get credit for Neosporin or fungal ointments This isa afoot parasitic diseaseifthat high fevers, shaking chills, flu-like symptoms, and anemia.if the This is caused by Consult care specialist you involves see no improvement after two weeks of using over-the-counter remedies, infection is severe a parasite that is transmitted from one human to another by the bite of infected Anopheles mosquitoes. In humans, the (the skin is red, itchy, peeling, or blistered), or if you have diabetes or some other circulatory problem. parasites travels to the liver, where they mature and release another form, the merozoites. These enter the bloodstream and infect the red blood cells. Disease: Malaria +1 Microbe: Protozoan = Plasmodium Treatments: Malaria can be cured if treated while in its early stages. Treatment depends upon the type of malaria you have. The patient may also need kidney dialysis, blood transfusions, oxygen therapy, and intravenous (IV) fluids, depending upon the severity of symptoms. Other types of malaria are treated successfully with chloroquine, which is taken by mouth. The length of time anti-malaria drugs must be taken depends upon the type of malaria, the age of the patient, and how severe the symptoms are. Even with drug treatment, malaria symptoms can recur if the parasite remains in the liver. Patients who have recovered from the P. vivax or P. ovale strains of malaria can take a drug called primaquine to prevent reoccurences of the disease. Station 6: Microbe Mission This station will need four microscopes- 2 prepared slides of human blood, and two blank slides and coverslips OR prepared hair (human or animal should work) Using the microscope at your station, focus on the components of blood- both the red blood cell and the white blood cell. Sketch their appearance below. Include their approximate size. Sketch of RBC +1 slightly smaller than WBC 6-10 micrometers Sketch of WBC +1 Lighter smaller ones- are the RBCs; darker purple ones that are larger and fewer- are the WBCs The diameter of a hair, which is 40-50 microns wide-=diameter ~ 15-20 microns 7-9 micrometers +1 Station 7: Microbe Mission This station does not need any supplies. Growth curves; graph interpretation Add in the following words to the graph below at the correct locations for bacterial growth (within the boxes). Stationary or Stable; Exponential; Death; Lag +4; then explain in words what is happening at each of these stages of growth Lag – relatively short period of time when population size is still make- going from 1 to 2 to 4 to 8 to 16 Exponential- rapid growth Stationary/Stable- birth and death rates relatively constant- population size remains stable – around 109 cells/ml Death- bacteria reach their carrying capacity and begin to die (run out of food or other habitat requirement) +3 points for fungi, algae (protist) and (protists or protozoans) Station 8: Microbe Mission This station will need 2 microscopes- and 3 prepared slides of each type- with the labels covered with masking tapelabeled “A”, “B” and “C” Using the microscope- identify the general shape of the bacteria found at each of the three microscopes- A, B & C +_3 “A” =______Bacilli or bacillus “B” = ______cocci or coccus “C” =_____spirilla or spirillium +5 Candida albicans (thrush) _dangerous____ type of microbe:__fungus Causes infection in humans- in the mouth Lactobacillus helveticus __beneficial__________ type of microbe: ___bacteria Makes dairy products such as yogurt Rhinovirus ___dangerous________ type of microbe: ____virus___ Causes the common cold in humans Agaricus bisporus ____________________fungus Fungus-edible mushrooms Geobacter metalloreducens bacteria Bacteria that can reduce hazardous metals- or bioremediate (clean up environmental pollutants)