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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
FIRST SEMESTER – APRIL 2014
BT 1827 - MICROBIOLOGY
Date : 04/04/2014
Time : 09:00-12:00
Dept. No.
Max. : 100 Marks
PART- A
(20 marks)
Answer all questions
I Choose the correct answer:
(5 x 1 = 5)
1. The type of bacteria that require low levels of oxygen for growth but are damaged by normal
atmospheric oxygen level are known as
a) facultative
b) microaerophilic
c) aerobic
d) anaerobic
2. The term that is used to differentiate the strain based on serological properties is
a) biovar
3. The F factor is
a) chromosomal DNA
b) serovar
b) a plasmid
c) morphovar
c) a virus
d) structovar
d) a bacteriophage
4. Viruses sensitive to ammonium sulphate are purified by precipitating with
a) Polyvinyl propionate
b) Polyethylene chloride
c) Polyethylene glycol
d) Polyvinyl chloride
5. Antibodies are ______________.
a) vitamins
b) carbohydrates
c) proteins
d) lipids
II State whether the following statements are True or False; if false, give reason: (5 x 1 = 5)
6. In Bacillus megaterium sporulation is completed in 5-6 hours.
7. Kuru is found in the eastern New Guinea tribe.
8. Sex Pili participate in the transfer of DNA from one bacterium to another.
9. Rubella virus grows well in chorioallantoic membrane of the embryonated eggs.
10. Animal virus has receptor sites that attach to the attachment sites on the host cell surface.
III Complete the following:
(5 x 1 = 5)
11. Phenol is also known as ____________ acid.
12. Organisms that obtain energy by the oxidation of electron donors in the environment are
called ______________.
13. ________________ viruses multiply in the host cell cytoplasm.
14. The RNA within the virion is called _________ strand because it acts as mRNA.
15. ICTV is ___________________.
IV Answer the following each within 50 words only
16. Distinguish between sterilization and disinfection.
17. What is gluconeogenesis?
18. What is the role of CD4+T cells in HIVinfection?
(5 x 1 = 5)
19. Write the properties of an industrial microbe.
20. What is cytopathic effect? Mention its symptoms.
PART- B
Answer the following, each within 500 words;
Draw diagrams wherever necessary:
(5 x 8 = 40 marks)
21. (a) Give an account of moist heat sterilization. Compare it with dry heat sterilization.
OR
(b) Explain the stages of endospore formation in Bacillus megaterium.
22. (a) Discuss the nutritional types of bacteria with examples.
OR
(b) Explain the growth curve pattern in bacteria.
23. (a) Give an account on generalized transduction.
OR
(b) Describe the process of penicillin production and mention the ways to increase its yield.
24. (a) What are prions? Explain the assumptions and diseases associated with them.
OR
(b) Explain the serological methods used for purifying virus.
25. (a) Give a comparative account on the biosynthesis of DNA and RNA viruses.
OR
(b) Give a brief account on the replication of TMV.
PART - C
Answer any two of the following, each within 1500 words;
Draw diagrams wherever necessary:
26. Give an account on bacterial conjugation.
27. Explain photosynthesis in cyanobacteria.
28. Explain the different methods of cultivating viruses.
29. Describe the multiplication pathways of RNA viruses with examples.
(2 x 20 = 40 marks)