Download Replication_ Transcription_Translation worksheet

Bio260 – Spring 2014
North Seattle College
DNA Replication, Transcription, and Translation to make a new cell!
PRECURSOR METABOLITES ARE USED TO MAKE SUBUNITS! Understand that all of those
precursor metabolites made by the central metabolic pathways can be used to make:
Amino acids, nucleotides, monosaccharides, fatty acids, and glycerol!
BUILD MACROMOLECULES: In order to make a whole new cell, the bacterium needs to put together the
subunits it just made into macromolecules so that it can use those macromolecules to build new cellular
structures!
 Part A: Fill out the following table:
Precursor
Metabolites
Subunits
Macromolecules
Cellular Structure made out of each
macromolecule
amino acids
nucleotides
monosaccharides
fatty acids and
glycerol
 Part B: Building Macromolecules
o Replicate the DNA chromosome for the new cell
 DNA Replication
• Enzymes: DNA gyrase, DNA ligase, DNA polymerases, Helicases, Primase
• Process: Origin of replication, Primer, Lagging strand, Okazaki Fragments, Leading
strand
• Subunits: DNA nucleotides (G, A, T, C)
o Make mRNA
 Transcription
• Enzyme: RNA polymerase, Sigma Factor
• Process: (-) strand, (+) strand, Promoter, Terminator
• Subunits: RNA nucleotides (G, A, U, C)
o Make proteins
 Translation
• Site of protein synthesis: Ribosome (made of rRNA and protein)
• Process: tRNA, mRNA, start codon (AUG), stop codons, reading frame, anticodon,
ribosome binding site
• Subunits: Amino acids
o Make lipids and polysaccharides: just know that many enzymes are involved!
BUILD A CELL! Finish off our story by explaining that those macromolecules will be used to make new
cellular structures which will eventually allow the cell to go through BINARY FISSION – making a
whole new cell!!
Now that new cell has to find some place to adhere and start all over again…..