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Molecular beacon fluorescence detection of pseudogene SHMT-psI using
two molecular beacon probes, E8/E10 and E9/E10
Evanescent Wave Sensing
Detection of Phosphorylated STAT3 Protein by Fiber-Based Sensor
Fluorescence Generated
at Surface
Exciting Laser
Cladding
Core
Fluorophore labeled
Phospho-STAT3 antibodies.
Fluorescence coupled
into fiber.
Phospho-STAT3
1500
Fluorescence intensity in a.u.
Phospho-STAT3 antibodies (IgG)
Evanascent wave excited
Alexa-430 Spectra in two different probes.
1200
Signal from stimulated Cells
Signal from non-stimulated Cells
900
600
300
0
400
STAT - Signal Transducer and Activator of Transcription protein
450
500
550
600
Wavelength in nm
650
700
Surface Plasmon Sensors
Kretschmann (ATR) geometry used to excite surface plasmons
Surface plasmon resonance curves. The left-hand side curve is for just the silver film (labeled
Ag); the right-hand side shows the curve (labeled Ag/p-4-BCMU) shifted on the deposition of a
monolayer Langmuir-Blodgett film of poly-4-BCMU on the silver film
Photonic Crystal Sensors
Close packing of colloidal nanospheres to form a photonic crystal of close-packed
colloidal array. (Left) Atomic Force Microscope (AFM) image of the surface layer.
(Right) Scanning Electron Microscope (SEM) image of a cross-section (Markowicz
and Prasad; unpublished).
Visible extinction spectra showing how diffraction depends on the glucose concentration for a 125µmthick PCCA glucose sensor. The ordinate is given as –log T, where T is the transmittance. The PCCA
expands for concentrations between 0.1 and 0.5 mM glucose.
FLOW CYTOMETRY
Basic concepts:
- Use molecularly specific fluorescent tags to label cells
- Single-file flow of cells (hydrodynamic focusing)
- Laser illumination of single cells
- Multiparameter fluorescence detection
-Data analyses
and/or sorting
ACIS, November 2003
Schematics of a five parameter flow cytometer showing det
of collection scheme.
Various parameters monitored
ACIS, November 2003
Clusters of cells represent distinct populations of cells
ACIS, November 2003
Applications
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HIV Monitoring
Leukemia or Lymphoma Immunophenotyping
Organ Transplant Monitoring
DNA Analysis for Tumor Ploidy and SPF
Primary and Secondary Immunodeficiency
Bacterial contamination in food and water
Biophotonics Opportunities
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Modeling of light propagation in tissue
In-vivo Bioimaging, Spectroscopy, and Optical Biopsy
Real-Time monitoring of Drug Interactions
Optical Biopsy
Nano-Biophotonic Probes
Multiphoton Processes for Biotechnology
Femtolaser based biotechnology\
Nanoarray multianalytic sensors
Multiparameter Molecular Flowcytometry
Single Molecule Biofunctions
Nanomedicine
Acknowledgements
Researchers at the Institute:
Prof. E. Bergey
Prof. A. Cartwright
Prof. M. Swihart
Prof. E. Furlani
Dr. A. Kachynski
Dr. A. Kuzmin
Dr. Y. Sahoo
Dr. H. Pudavar
Dr. T. Ohulchanskyy
Dr. D. Bharali
Dr. D. Lucey
Dr. K. Baba
Dr. J. Liu
Outside Collaborators
Prof. R.Boyd
Prof. J.Haus
Prof. J M J Frechet
Prof. M. Stachowiak
Dr. A. Oseroff
Dr. R. Pandey
Dr. J. Morgan
Dr. P Dandona
DURINT/AFSOR
 Dr. Charles Lee
“Lighting the Way to
Technology through Innovation”
The Institute for Lasers, Photonics and
Biophotonics
University at Buffalo
Biophotonics
P.N.Prasad
www.biophotonics.buffalo.edu