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Origins of Life: Formation of The Earth • The earth is 4.6 bya. How did it form? • As large clouds of gases cooled, gravity pulled minerals, asteroids, rocks together forming the earth Earth is composed of: • Crust-outer layer made of basalt, granite • Mantle-intermediate density layer of rock • Core-inner layer of molten nickel, iron Earth’s First Atmosphere A mixture of gases: H2, N2, CO, CO2 No O2 present H2O present after the crust cooled, and rains began, first seas formed Without the presence of H2O, cell membranes would not have formed; no membranes, no cells Lipids are known to spontaneously form bilayered vesicles in water Origin of Life: Synthesis of Organic Compounds (monomers) • All living cells are made of • • • • carbohydrates, proteins, nucleic acids, lipids How did they form initially? Stanley Miller Experiments Combined CH4, NH3, H2O, H2 in glass apparatus under a vacuum and an electrical spark Amino acids spontaneously formed in less than a week (amino acids proteins) http://www.npr.org/templates/story/story.ph p?storyId=19308778 Origin of Life: Synthesis of Organic Compounds: Miller Experiments Products of Miller experiment reactions: Formaldehyde, Cyanide gas • Formaldehyde glucose, ribose, deoxyribose (DNA, RNA) • Formaldehyde porphyrin ring chlorophyll A (photosynthesis) • Cyanide gas adenine base (DNA, ATP, NADPH, NADH) • Lasting bonds could form near hydrothermal vents on sea floor (peptides) in clay templates (polypeptides) Which came first, Proteins or RNA? Protein first hypothesis: amino acid polymerize abiotically; enzymatic properties selected; DNA followed enzyme formation- enzymes needed for DNA replication and RNA/nucleotide formation RNA first hypothesis: RNA can function like enzymes self replicating system; RNA genes would have directed and enzymatically carried out protein synthesis Origins of Plasma Membranes Lipid bilayer (Fluid Mosaic Model) composed of phospholipids: hydrophilic head + 2 hydrophobic tails Protects, selectively permeable When amino acids are heated and cooled, they form a microsphere that is selectively permeable The spheres can incorporate free lipids and in presence of H2O form bilayer Origins of Organelles • Specialized structures with specific intracellular functions: mitochondria, chloroplasts, RER, SER, golgi, etc. • Folding of PM inwards may have given rise to the nucleus and ER • Endosymbiosis-one bacterium engulfed another and it survives, both benefiting from the relationship – Mitochondria/chloroplasts have own DNA – Mitochondria/chloroplasts resemble certain bacteria in size/structure – Mitochondria/chloroplasts divide by binary fission – Outer membrane and inner membrane of Mitochondria/chloroplasts are different, inner resembles bacteria, outer resembles eukaryotic cell Origins of Self Replicating Systems and First Cell • Abiotic synthesis of small organic molecules (monomers) • Monomers join to form polymers (clay/vents) • Aggregation of polymers inside a plasma membrane with enzymatic properties= protocell • Stability of DNA and selection for genetic code creates a self-replicating system of life History of Life: Fossils Buried remains and mineralized impression of organisms from the past; older fossils deeper sediment layers (stratum) due to sedimentation: weathering and erosion of rocks produce accumulation of particles Fossils • Fossil age is determined through Radiometric (absolute) datingmeasures the amount of isotope in new rock compared with the isotope remaining in old rock; unstable radioisotopes will decay to a more stable form with time • Index fossils: relative dating methods used to identify deposits made at the same time in different parts of the world Fossils • half life-amount of time it takes for half the isotope to convert to a more stable form; every isotope has its known half life • Radioisotope decay is constant; it does not depend on pressure, temperature • 14C has a half life of 5700 years. If your fossil is 60000 years old, how many half lives occurred? Radiometric Dating Problems 4 half lives occurred. 60000/5700=10.5 13N has a half life of 25000. How old is your fossil if 4 half lives have occurred? If you have only 1/8 of your radioisotope left, how many half lives have occurred? (1/2n), n = half life Tree of Life • 3.5 bya anaerobic prokaryotic • • • • • cell Archaebacteria and bacteria lineages 3.4 bya divergence between Archae- bacteria and eukarya 3.2 bya photosynthesis (O2 increases) 2.5 bya cellular respiration 2.1 bya eukaryotic cell Early eukaryotes Fungi, Plantae, Animalia How old is the Earth? Using Uranium 238, earth is 4.6 billion years old Precambrian Time • Stromatolites-rocks dating almost 3.5 bya (contained prokaryotic cell similar to cyanobacteria) • Living stromatolites have surface covered with cyanobacteria Snowball earth: http://www.youtube.com/watch?v=mX3pHD7NH58 Paleozoic Era (570- 240 mya) Pangea formed Organisms of major lineages formed in oceans (Cambrian explosion) Major ice age-70% of all marine organisms became extinct Invasion of Land; emergence of vascular plants, fungi, invertebrates, insects, fish, amphibians, reptiles Paleozoic Era (570-240 mya) “Great Dying”-90% of all known species lost; volcanic eruption, increase in gases, temperature Pangea breaks apart, forming Gondwana and Laurasia Paleozoic Era (570-240 mya) Carboniferous period forests began to turn to coal, source of biofuels we use today Mesozoic Era (240-65 mya) Pangea broken apart, Gondwana and Laurasia begin to break apart Gymnosperms, angiosperms, insects, reptiles dominant land organisms Mesozoic Era (240-65 mya) • First dinosaurs (Triassic Period), continued dominance for 140 my • 65 mya dinosaurs became extinct; Asteroid impact causing increase in temperature and CO2 gas Cenozoic Era (65 mya-present) Pangea completely broken apart Major land mass collisions forming Cascades, Andes, Himalayas, Alps Warmer and wetter climates Emergence of mammals as the dominant land animals Wooly mammoths, saber tooth tigers, horses, bear-dogs 40-5 mya Emergence of human ancestors, humans Evolution was Influenced by Movement of the Land Masses Continental Drift-movement of land masses/continents Plate tectonics-earth has slab like plates that are in constant movement. This movement is directed by the earth’s molten core. Many mountain ranges formed by the crashing and pushing up of these plates Mass Extinctions 5 major extinctions have occurred through history due to continental drift, changing temperatures, natural disasters