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Bacterial Comparisons Of Used and Unused Medication Vials Whitney Baker Undergraduate Student (Biology-Health Science Concentration) Biology Department Tennessee Tech University Cookeville, TN 38501 Project Summary Problem-Pharmacy Employees encounter many types of bacteria. Objective- To determine how many types of bacteria a used medication vial might harbor. Hypothesis- Used medication vials, that pharmacy employees touch, harbor more bacteria than unused vial originally released to the customers. Null Hypothesis- Used and unused medication vials contain the same amount of bacteria. Project Summary Cont. Goal-To determine the specific number of different types of bacteria present on a used vial. Goal Accomplishment- I plan to swab vials and use TSA plates to determine bacterial growth colony counts. Expected Results- I expect that used vials will have a great number of bacteria than unused vial, and therefore a greater colony count. Introduction Problem-Pharmacy Employees encounter many types of bacteria. Literature Review – – Karchmer, T.B., A.M. Anglim, L.J. Durbin, B.M. Farr. 2001. Pseudoepidemic of streptococcal pharyngitis in a hospital pharmacy. American Journal of Infection Control 29: 104-108 – Rusin,P., S. Maxwell, C. Gerba. 2002. Comparative surface-to-hand and fingertip-tomouth transfer efficiency of Gram-positive bacteria, Gram-negative bacteria, and phage. Journal of Applied Microbiology 93:585-592 Objective/Hypothesis Statements Objective- To determine how many types of bacteria are present on a used medication vial Hypothesis- A used medication vial harbors more bacteria than an used vial. Null Hypothesis – Used and unused medication vials harbor the same amount of bacteria. Methods and Materials Data Form Experimental Design Matrix Methods– Collect samples using aseptic techniques. – Avoid environmental contaminations. – I will be using the T-test as my statistical method. (Harper, 1967) Expected Results and Benefits Advantages- Knowing what Pharmacy employees come in contact with daily, and being able to improve handling techniques. Benefits- Less bacterial contact means less sickness. This will improve productivity and decreased work days missed. Project Timeline Project Budget