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-Hemolytic Streptococci Ali Somily MD,FRCPC,D(ABMM) Introduction Grouped either by : • A.phenotypic 1. 2. Hemolysis(,ß or ) Lancefield antigen – Cell wall CHO – A,B,C,D,Fand G ect Or B.Genotypic &ß Hemolysis Lancefield Agglutination -Hemolytic Streptococci Partial hemolysis of blood Green zoon around the colony Examples: • • • • S.Pneumoniae S.Viridans Enterococcus S.Bovis STREPTOCOCCUS PNEUMONIAE Aerobic extracellular Feature : • Gram Positive cocci in pairs or short chains(Lancet shape) • Colony :Gray –white variable on BAP Non motile Capsule : Polysaccharidemore than 80 types Note : No Glycocalyx , No Exotoxin Virulence Factors Capsule: Polysaccharide (resist phagocytosis IgA Protease:Prevent Opsonization by IgA at Mucous Membrane Adhesion: Mediates attachement of S.pneumoniae to Epithelial Cell Autolysin/Pneumolysin Quellung Test (AB’s swelling of capsule CULTURE BAP; 5-10%CO2 -hemolytic Mucoid (capsule) SR Concave (punched out/collapse) Laboratory Tests Catalase : -ve Hemolysis : Alpha 6.5% Nacl : No growth CAMP Test : -ve Bile Esculin: -ve Bile Solubility : +ve Optochin :Sensitive Lancefiield : None (CHO C) IDENTIFICATION Bile solubility (NaDC) Optochin S (disk 5g&6mmzoon>=14 mm) Source and Transmission Normal Flora of Upper Respiratory Tract in 20-40% of people Horizontal Transmission via Droplet and Inhalation Pulmonary infection due failure of Muccocilliary action AlveoliLobe Meningitis after Sinusitis , Otitis Media or Bacteremia through Choroid Plexus Clinical Primary infection • Community Acquired Pneumonia • Bacteremia • Endocarditis • Meningitis • Localized Sinusitis O.M Secondary Infection • • • • Non-capsulated Opportunistic infection Lungs only Impair or poor ciliary activity Viral, Smoking, dust Lober Pneumonia Adult and Sickle Cell Disease Fever , cough(sputum), Dull on Percussion Can be fatal, Abscesses Diagnosis: Sputum GS and Culture Risk factor • Hyposplenism • • • • • • Splenectomy Asplenia Sickle Cell Diseases Liver disease Hypogammaglobinaemia Alcoholism Cigarette smoking Viral Infection Malnutrition Meningitis Adult and Elderly Symptoms: fever, neck Pain,Neck rigidity Medical Emergency Lumbar Puncture PMNs , Protein, Glucose and Cloudy Direct Extension : Sinises,OM or Through Blood Sinusitis and O.M Sinusitis : S.pneumoniae most common cause, follow allergy or viral infection O.M : S.pneumoniae most common cause, follow allergy or viral infection which prevent eustachian tube drainage. Host Defense and Immunity IgG Antibodies : Type specific immunity Classical Pathway Immunity: C1 activated by capsule: Antibody -dependent Opsonization Alternative Pathway Complement Antibody -independent Opsonization C5a complement : chemotaxis attract PMNs Vaccine :Immunity for few years Treatment and Prevention Treatment • PenicillinG ↑ resistant recently due to PBP alternation • Ceftriaxone for meningitis • Ceftriaxone +/-Vancomycin and or Rifampicin Vaccination • Polsaccharide capsule • Conjugate vaccine • Indication Children SCD Splenectomised patient HIV Elderly Cardiopulmonary and renal diseases VIRIDANS STREPTOCOCCI Streptococcus Viridans Group 1. 2. 3. 4. Mitis Mutans Salvarius Angionosis Extracellular aerobic Gram positive cocci in chains and pairs Gray-white variable colony on BAP No exotoxin Virulence Factors Dextran exopolysaccharide glycocalx: • Provides means of adherence to defective hearts valves • May block the action of antibiotics Lipoteichoic Acid (LTA): mediates adhesion to fibronectin in clots on defective heart valves Glucan: Polysaccharides made by S.mutans from sucrose in the mouth , they provide a mean of attachement to teeth enamel. Other Acids: Made by S.mutans from fermentation of sugars in the mouth contributed to tooth decay Example of A biofilm Formation of dental plaque by Streptococcus mutans • bacteria adhere to the tooth by a protein on the cell surface, grow and synthesize a dextran capsule • binds the bacteria to the enamel and forms a biofilm 300-500 cells of thickness • bacteria can cleave sucrose to glucose + fructose • glucose is polymerized into an extracellular dextran polymer that cements the bacteria to tooth enamel and becomes the matrix of plaque • this dextran slime can be depolymerized to glucose for use as a carbon source, resulting in the production of lactic acid within the plaque that decalcifies the enamel and leads to dental caries Laboratory tests Catalase : -ve Hemolysis: Alpha 6.5% NaCl : No growth Bile Esculin : -ve Bile Solubility : -ve Optochin : Resistant CAMP Test : -ve Lancefield ; Non (CHO C) Clinical Normal Flora in the Oropharynx ,GIT and GUT, enters blood after dental work or due to poor oral hygiene Bacteremia : S.mutan . Sub-acute Endocarditis: most common cause , after bacteremia due to dental work and infect maily abnormal valve or prosthetic valve , rarely normal valves. It is fatal if not treated. Dental caries: see above. Lysis of bacteria by serum enzyme and lysosomal enzyme. No vaccine available Treatment Dental prophylaxis : One hour before procedure in case of abnormal valve with ampicillin Ampicillin +/- aminoglycoside in case of endocarditis Vancomycin in penicillin allergic patient Treatment VGS, NVS, sreptococcus MIC <0.1 ug/mI MIC >0.1 —0.5 ug/mI Native valve prosthetic valve PenG PenG 6wk +Gentamicin 2wk PenG 6wk + Gentamicin 4wk PenG 4wk +Gentamicin 2wk Enterococcus Fecal strep separated genus/by molecular Enterococcus Faecalis and E.Faecium Extracellular Aerobic Gram positive cocci single in chains or pairs Gray –white or variable colony on BAP Non Motile, Not capsulated, no Glycocalx and No Exotoxin Adhesion to defective heart valves and urinary tract Antibiotics resistant Laboratory Tests Catalase : -ve Hemolysis: Alpha, Beta or Gamma 6.5% NaCl : Growth PYR : + ve and LAP : +ve Growth at 45 oC 40% Bile Salt: +ve Bile Esculin : +ve CAMP Test : -ve Lancefield ; group D (CHO C) Source and Transmission Normal Flora in GIT in human Harsh condition Abiquitous / soil,water,plants, GIT, GU human 15 Spp/E.faecalis80-90% of clinical isolate Bacteremia after urinary tract infection, Intraabdominal route or via indwelling catheters Exogenous acquisition in the hospital (nosocomial) Clinical Urinary tract infection (UTI) : Nosocomial, upper and lower UTI Bacteremia: From UTI , Intra-abdominal infection or indwelling catheter ( Intravenous or hemodialysis) , common in I’C patients Sub-Acute Endocarditis : After bacteremia, affects abnormal or prosthetic valves , it is fatal if not treated Host defense and immunity is unknown Treatment and prevention Ampicillin in case of UTI by E.faecalis Vancomycin in case of E.faecium Ampicillin or Vancomycin + gentamicine in case of endocarditis Streptogramin or Linazolid in case of Vancomycin Resistant Enterococcus (VRE) Infection control measures in case of VRE outbreak No vaccine available Endocarditis Enterococcus, MIC >0.5 ug/ul, Native valve Prosthetic valve PenG or Amp total 6 wk plus Gent for 4-6 wk Streptococcus Bovis ( Streptococcus gallolyticus NEW NAME) Group D streptococci Aerobic extracellular Gram positive cocci in chains or pairs Gray-white colony on BAP Non-Motile, Non-Capsulated and Glycocalyx No Valulant factors Laboratory Tests Catalase : -ve Hemolysis: Alpha, Beta or Gamma 6.5% NaCl : No growth (opposite to enterococcus) PYR : -ve (opposite to enterococcus) No Growth at 45 oC (opposite to enterococcus) 40% Bile Salt: +ve (opposite to viridans) Bile Esculin : +ve (opposite to viridans) CAMP Test : -ve Lancefield ; group D (CHO C) Two biotypes I &II Clinical Normal Flora in GIT Infection after diruption of GI epithelium in case of malignancy Bacteremia from GIT Endocarditis after bacteremia, fatal if not treated Colonic cancer has strong association with S.bovis bacteremia IgA, IgG and PMNs Treatment penicillin or vancomycin( rarely resistant to vancomycin) No vaccination available Summary + +