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Bacteria and Viruses Ch. 19 Bacteria: They’re everywhere! Size of Bacteria • Coin 1cm in diameter • Size of bacteria 1-5 micrometers • 2000-10,000 bacteria lined up • Penny 1.9 cm: How many bacteria IN A LINE? • Quarter 2.5 cm? DNA arrangement in Prokaryotes • What is this circular DNA called? • Plasmid • Prokaryotes do not have an organized nucleus or organelles. Classifying Prokaryotes • Eubacteria (true bacteria) –cell wall contains the carbohydrate, peptidoglycan. • Archaebacteria (ancient bacteria)- lack peptidoglycan in cell walls and have different membrane lipids. The DNA sequences of key archaebacteria are more like those of eukaryotes than eubacteria. They live in harsh environments. Identifying Prokaryotes • Shape – – – – – Rod (bacilli) Spiril (spirilla) Circluar (cocci) Chain (strepto) Cluster (staphlo) • Movement - flagella, slide, non moblie, swivel • Cell Walls – Gram +: thick peptidoglycan wall (purple) – Gram -: thin layer of peptidoglycan inside the lipid bilayer (pink) Examples of pathogenic bacteria Gram + or Gram – Identify shape Metabolic Diversity- another way to classify • Heterotrophs- can’t make their • Autotrophs- make own food own food. – Photoautotrophs- plants – Chemoheterotrophs- taken in photosynthesis organic molecules for both – Chemoautotrophs- make energy and a supply of C. US. organic compounds from – Photoheterotrophs- use sunlight CO2. H2S vents for energy, and need to take in – Which category do most organic compounds as C prokaryotes fall under? source. Ex: purple non-sulfur bacteria, heliobacteria Oxygen or no oxygen? • Which of these test tubes show bacteria growing in an anaerobic environment? • Why? • Which of these show bacteria living in an aerobic environment? • Why? Releasing Energy • Obligate aerobes- require oxygen Ex: TB • Obligate anaerobes- require no oxygen Ex: Clostridium botulium • Facultative anaerobes- do not require oxygen, but are not killed by its presence. Ex: E. coli Which do you think were the first type? Reproduction • How do bacteria reproduce? • Binary Fission • What type of reproduction is this? • asexual • What is the end result? • Bacteria that are identical to the parent. Other forms of reproduction • Spore formation• Conjugation- hollow occurs when growth bridge forms between conditions are two bacterial cells, and unfavorable. An genes move from one endospore is formed cell to the other. to protect DNA and cytoplasm. When conditions improve, endospore will germinate. Bacteria Importance • Decomposers • Nitrogen fixersconvert N into usable form. Rhizobium, nodules on roots of legumes (bean plants) • Human uses: – Food, remove waste products, digestion, clean up oil spills. The bacterial cell • What is the flagellum used for? • Movement • What are the three shapes of bacteria? • Cocci, bacillus, spirillum Bacteria Environments • Where do bacteria live? • Everywhere, they’re ubiquitous • What do we call the bacteria that live in cold temperatures? • psycrophiles • What do we call the bacteria that live in hot temperatures? • Thermophiles • Bacteria that live in very acidic environments are called acidophiles. • What do we call those bacteria that live at “normal” temperatures? • Mesophiles Where bacteria grow, where nothing else can. • What do we call bacteria that live in swamps? What do they make? • Methanotrophs make methane gas. • What are bacteria called that live in extreme salt conditions? • Halophiles Viruses • Particles of nucleic acid, protein and in some cases lipids. • Nucleic acid (DNA or RNA) surrounded by protein capsid that protects the NA. • Nonliving Viral Diversity • DNA or RNA • Single stranded or double stranded • Enveloped (animal) or non-enveloped (plants) • Temperate or virulant • Symmetry: isohedral (many facets) or helical (round) Viral Infection • Lytic- virus enters a cell, makes copies of itself and causes the cell to burst. • Destroys host cell. • Lysogenic- virus integrates its DNA into the DNA of host cell, and the viral genetic information replicated indefinitely along with the host cell’s DNA. • Prophage- viral DNA that is embedded in the host’s DNA. Retroviruses • Contain RNA. • Produce a DNA copy of their RNA and insert it into the DNA of host cell. • RNA DNA using reverse transcriptase. (RT) • AIDS Vector • Viruses must have a vector or living host in order to grow and reproduce. – Deer tick and Lyme Disease, mosquito and Malaria • Viruses are much smaller than bacteria. Pathogens • Viruses or organisms that causes an infectious disease. • Ex: viruses, bacteria, protists, fungi, roundworms and flatworms. • Bacteria- some produce toxins • Virus- chicken pox, cold • Protists and roundwormsdestroy body tissue by feeding on and burrowing into the tissue. • Fungi- athletes foot, ringworm Remembering Invaders • Antibiotics – Slow down microbial reproduction: bacteria, fungi and protists • Vaccinations- Edward Jenner • Injections of weakened or killed microbes to enhance immunity – Stimulate development of memory cells Viroids and Prions • Viroids- contain only RNA, but lack an envelope and capsid. • Cause problems in potatoes, tomatoes, and fruit trees. • Prions- proteins that have the ability to transmit diseases. • Protein direct the host to create abnormal proteins that can cause serious neurological disease in animals and humans. • Mad Cow Disease • Alzheimers ? Bacteria and Viruses Review • Go to the following link and click on your book. Go to Ch. 19 and take the Self-test and review the concepts. • Ch. 19 Review • Be a microbe detective.