Download Microscope

Microscope
Important Lab instrument
Why use a microscope?


To view objects and detail too small to see with
human eye. Improves resolution of object
List examples of how what you used it for:
 Blood

cell detail
rbc, wbc, platelets
 Bacteria

Cocci, rod, bacillus
 Protozoa


Trichomonas
Giardia
Types of Microscopes

Compound microscope
 Bacteria,

Electron microscope
 Required

fungi and protozoa
for viruses
Fluorescence
 Used
as a diagnostic tool for
immunofluorescence tests
Parts of a microscope

Coarse adjustment
 1st
step in focusing to change the distance
between specimen and lens

Fine adjustment
 To
fine tune the picture
 Used particularly with 100x and oil objective

Stage
 Holds
the slide, moved up and down with
coarse and fine adjustment
Parts of a microscope

Objectives
 Common
objectives are 4x, 10x, 40x, 100x, oil
 Total magnification is eye piece magnification
multiplied times objective you are viewing with

40x objective and 10x eye piece is 400x
magnification
 Oil
objective is labeled and is always used
with immersion oil

Oil increases the resolving power by focusing the
light rays
Proper care for microscope
Always start with lowest objective to focus
 Always store with lowest objective locked
in place
 Carry with two hands
 Cover with dust cover
 Be sure to clean oil off of oil objective
 Use fine adjustment with 100x and oil
objective

THE COMPONENTS OF THE
MICROSCOPE
What do you remember?
THE MICROSCOPE



EYEPIECE
What is it
when there is
only 1?
When there
are 2?
THE MICROSCOPE
 ARM
THE MICROSCOPE
 BASE
THE MICROSCOPE

TUBE
THE MICROSCOPE
 REVOLVING
NOSEPIECE
THE MICROSCOPE




OBJECTIVE
LENS
What are the
magnification of the 4
objectives?

4, 10, 40, 100x

10x unless otherwise
stated
What magnification is the
eye piece?
What would be the total
magnification when
viewing an object
through the 40x
objective?

400x
THE MICROSCOPE
MECHANICAL
STAGE
 Why is a
mechanical stage
better than using
clips?

THE MICROSCOPE
IRIS
DIAPHRAGM
 What does
this do?

 Control
the
amount of light
THE MICROSCOPE
COARSE
ADJUSTMENT
KNOB
 What 2 objectives
do you use the
coarse adjustment?

4
and 10x
THE MICROSCOPE
 FINE
ADJUSTMENT
KNOB


What objectives
is this used
with?
All, but mostly 40,
100x
THE MICROSCOPE
Light
THE MICROSCOPE
 ON/OFF
SWITCH
CARE OF
THE MICROSCOPE

Always carry the
microscope by
the arm and the
base and use 2
hands!
CARE OF
THE MICROSCOPE

Wrap the cord up
or around the
microscope so it is
not hanging down.
CARE OF
THE MICROSCOPE
Always
lower the
stage and
clean all
objectives
off
 Always use
a dust
cover!
