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starter
Unscramble each of the clue words. Take the letters that appear in
and unscramble them for the final message.
C L I M A T E
C H A NG E
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Learning objectives
 How is nitrogen recycled in
ecosystems?
 What do you understand by
the terms ‘ammonification’,
‘nitrification’, ‘nitrogen
fixation’ and ‘denitrification’?
 Where does nitrogen enter
the living component of the
ecosystem?
 Where does nitrogen enter
the n0n-living component of
the ecosystem?
Success criteria
 Be able to construct a flow
diagram of the nitrogen cycle
in sufficient detail to
illustrate the processes of
ammonification,
nitrification, nitrogen
fixation and denitrification.
 Successfully answer an
examination style question.
What happens to nutrients?
Nutrients are constantly recycled through the carbon cycle and
the nitrogen cycle.
Producers
nutrients in
the soil and
atmosphere
energy and
biomass
energy and
biomass
microbial decomposition
of dead organisms and waste
Decomposers
Consumers
Why is nitrogen so important?
Nitrogen is essential for growth because it is used by plants
and animals to make proteins.
Nitrogen makes up about 78% of the
atmosphere. However, nitrogen
deficiency is the most common
cause of poor plant growth.
Why are plants unable to use the
nitrogen straight from the air?
Nitrogen gas (N2) is unreactive and is not easily converted into
other compounds. Most plants can only take up nitrogen (by
active transport) in the form of ammonia or nitrate.
How is atmospheric nitrogen changed into a useable form?
Nitrogen Fixation
 Nitrogen gas can be ‘fixed’ into
ammonia by humans using
the Haber process. This is then
used in the production of
fertilisers.
 A small amount of nitrogen is
fixed to nitrate by lightning.
Nitrogen Fixation
 Nitrogen fixing bacteria reduce
nitrogen gas to ammonia, which
they then use to manufacture
amino acids. Nitrogen rich
compounds are released from them
when they die.
 Nitrogen fixing bacteria may be
free living in soil or water.
Non-symbiotic
nitrogen fixing
bacterium
Azospirrillum.
Nitrogen Fixation
 The nitrogen-fixing bacteria may live in colonies inside
the cells of root nodules of leguminous plants such as
clover or peas (mutualism).
In return for sugars
from the plant, the
bacteria fix
nitrogen which can
be used by the
plant for growth.
The nitrogen in the plant proteins is passed on to animals
through food chains.
Ammonification
 When living organisms excrete waste or die their nitrogen is
returned to the soil in the form of ammonium compounds
by saprobiotic microorganisms (bacteria and fungi).
 Microbial saprophytes break down proteins in detritus to
form ammonium ions (ammonification or deamination).
 This is where nitrogen returns to
the non-living component of the
ecosystem.
Nitrification
 Plants can only take up nitrogen in the form of nitrate.
 Ammonium compounds are oxidised into nitrates by
nitrifying bacteria in two stages:
 first forming nitrite ions (Nitrosomonas)
 then forming nitrate ions (Nitrobacter)
 This is an oxidation reaction and so releases energy.
 These are chemosynthetic bacteria, which means they
use the energy released by nitrification to live.
Nitrification
 Nitrifying bacteria require oxygen
to carry out these conversions so
the soil needs many air spaces.
 Farmers can increase the aeration
of soils by
 Ploughing
 Good drainage
Denitrification
 The anaerobic denitrifying bacteria convert nitrate
to nitrogen gas , which is then lost to the air.
 These are present in greater
numbers when the soil
becomes waterlogged.
 This represents a constant loss of “useful” nitrogen
from soil, and explains why fertilisers and nitrogen
fixation by the nitrifying bacteria are so important.
The nitrogen cycle
 What does your diagram look like?
 Did you meet our success criteria?
 Be able to construct a flow diagram of the nitrogen cycle
in sufficient detail to illustrate the processes of
ammonification, nitrification, nitrogen fixation and
denitrification.
 What does the person sat next to you think of your
work?
 If in doubt see me for another blank and fix it?
Plenary - Exam question
1.(a)Wet moorland soils often contain low concentrations of nitrogen
compounds, as a result of denitrification. Sundew is a plant which lives in
wet moorlands. Its leaves have sticky hairs which can trap small insects
that are then digested.
(i)Describe the process of denitrification
(2)
(a)(i)Conversion of nitrates/any nitrogen compound to nitrogen (gas);
by (denitrifying) bacteria;
(2)
(ii) Explain how digestion of insects helps the sundew to obtain additional
nitrogen compounds.
(2)
(ii) insects contain proteins;
Digested to amino acids;
which can be absorbed/diffuse into leaf
2 max
Plenary - Exam question
(b)Samples of plant and animal tissue were analysed to determine the proportions of the
elements, carbon and nitrogen.
In the plant tissue the ratio of carbon to nitrogen was 40:1.
In the animal tissue the ratio was 8:1.
Explain why the ratio is much higher in the plant tissue than in the animal tissue. (2)
(b)High proportion of carbohydrate/starch in plant tissues;
Cellulose (in cell walls);
More protein in animal cells (such as muscle);
2 max
(c)Describe how nitrogen in compounds in a dead plant is made available for use by
other plants.
(6) (Total 12 marks)
(c)Proteins/amino acids broken down;
by saprophytes/decomposers;
ammonification (deamination)/ammonium compounds/ammonia formed;
Ammonia converted to nitrate;
by nitrifying bacteria;
Nitrite as intermediate;
Nitrate can be absorbed by roots;
6 max
[12 marks]
Learning objectives
 How is nitrogen recycled in
ecosystems?
 What do you understand by
the terms ‘ammonification’,
‘nitrification’, ‘nitrogen
fixation’ and ‘denitrification’?
 Where does nitrogen enter
the living component of the
ecosystem?
 Where does nitrogen enter
the n0n-living component of
the ecosystem?
Success criteria
 Be able to construct a flow
diagram of the nitrogen cycle
in sufficient detail to
illustrate the processes of
ammonification,
nitrification, nitrogen
fixation and denitrification.
 Successfully answer an
examination style question.