Download GK-12 Saturday Workshop - University of California, Irvine

GK-12 Saturday Workshop
December 3, 2011
Kirby-Bauer Method
• The Kirby-Bauer method is the most widely used antibiotic
susceptibility test in determining what treatment of antibiotics should
be used when treating an infection
• Bacteria are grown on agar plates in the presence of thin discs
containing relevant antibiotics. If the bacteria are susceptible to a
particular antibiotic, an area of clearing surrounds the disc where
bacteria are not capable of growing (called a zone of inhibition).
• If the organism is susceptible to a specific antibiotic, there will be no
growth around the disc containing the antibiotic. Thus, a “zone of
inhibition” can be observed and measured to determine the
susceptibility to an antibiotic for that particular organism. The
measurement is compared to the set criteria, the organism can be
classified as being Resistant (R), Intermediate (I) or Susceptible (S).
Lesson #1: Reducing Risk
Lesson #1: Reducing Risk
Summary
• Students will investigate the effects of
various antimicrobial solutions upon the
growth of bacteria by measuring the zone
of inhibition.
• We will collect data and analyze using
Excel.
• Based on their observations, students will
compare various methods for preventing
the growth and spread of microorganisms.
Lesson #1: Reducing Risk
Prior Knowledge and
Standards
• Students will have an introduction on
infectious diseases and methods of
prevention.
• Students will have been introduced to
bacteria and antibiotics.
• Standards addressed: 7.7 a-e, 7.1 a,
7.1 c
Lesson #1: Reducing Risk
Lab Handout
Lesson #1: Reducing Risk
Timeline
Day 1
• Introduce lab activity
• Demonstrate how to
set up a petri dish
and sterile
technique.
• Set up bacterial
plates with antibiotic
discs
Day 2
• Collect Data
• Review Concepts
• Conclusion
Questions