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Taming Cancer by Inducing Immunity via E. coli Presenter: Xu Nanfang Cai Danfeng Chen Yufei First, we need a E.coli that can escape the immune system after infusing into the blood Protect E.coli from Immune System K1:O16 capsule Idea from “bactoblood”, UCBerkeley, iGEM2007 polysialic acid is a frequent coating on mammalian cells, the human immune system does not recognize K1 as foreign Together, these surface modifications allow the bacterium to avoid detection by the immune system and should extend the serum half-life to several hours rather than the less-than-5 minutes observed with rough strains Both of these gene clusters have been installed into the genome of MC1061 in the course of preparing our chassis strain, MC828U Amazing O2 First role of O2: to locate the cancer cells Study shows that where there’s cancer existing, oxygen level is usually significantly lower than other parts of the body. E. coli is anaerobic Second role of O2: to act as a signal for the transcription of a series of factors. A a Densitysensing Oxygensensing K1:O16 hydrolysis A inv Petra Dersch and Ralph R.Isberg: A region of the Yersinia pseudotuberculosis invasin protein enhances integrin-mediated uptake into mammalian cells and promotes self-association Suicidal Component Make sure that E.coli kills oneself after several generations of division Advantages: Problems: 1. Dependent on the immune system of oneself 1. The efficiency of eliminating capsules 2. In vivo vs. in vitro 2. Detect cancer at an early stage 3. Cancer-specific