Download N.S. F. Grant, G-6174,

N.S.
F.
Grant,
California
Frost,
L.C.
spored
Such
used
strains
(albino),
Some
Every
ot
48
30’;
the
undergo
in all
each
observed
meiosis
at
meiosis
usually
number
spores
completed
the
and
crosses.
&
fewer
than
of spores
were
also
the
three
in
black
spores
one
ascus
No.
Meiosis
effect
of temperature
24
on the
25’,
crosses
I, many
asci
found
in
22 of which
several
asci
only
of normal
and
late
the
on
the
and
protoperithecia
reached.
incubated
at
for
25’
about
continuously
At 25”,
however,
fifth
day and the
from
25’
of aberrant
than
eight
asci
black.
Asci
mis-shopen
to 30’
asci
did
WCIS present
spores.
observed.
Never
more
The highest
with
than
fewer
eight
ascospores.
asci.
of osci:-
8-spored
Multi-spored
Less
than
8-spored
I.
8
25
0
0
I-14
7.2
4
7
8
IO
3.
l-13
7.0
0
2
IO
13
4.
2-14
8. I
I
6
IO
9
5.
4-15
9. I
5
8
13
4
Sample
l-4.
and
Samples
in some
cr,
cross:
+, + 0
meioses
at 30”.
during
highest
Cross:
5.
& Estimated
The
23
2.
* Samples
ceeding
8.0
l-5
frequency
of the
during
25’
each
bused
when
CT, al-2
(I)
on
cultures
all
25’
and
asci
the
+, al-2
x
change
to 30”
of aberrant
asci
x
occurred:
(3)
change
+
at
0
25’C.
to 30°
(2)
and
ot
clt 30’.
25’,
during
(4)
all
change
at
30’.
25”
(5)
at
to 30”
25”,
at 30’.
25 asci
appeared
were
selected
to occur
transferred
at
random.
in those
from
the
day.
transferred
multi-spored
aberrant
I
and
steadily
grossly
Norma
intervals
continues
sixth
3 were
medium
was
observation.
hours
and
Number
Average
batch
Barratt,
Eatches
of
of Westergoard
incubated
at
incubation
more
contained
W.
from
contained
the
R.
transferred
frequency
clsclJs:-
Range
Biology,
phenomenon
eight
spores
of development
a high
all
Dr.
Catcheside.
stage
in crosses
of any ascospores.
of spores
per
the
than
stage
were
late
at
at
commenced
However,
were
wets
In addition
I-
of
by
G.
reproductive
rest of the
the
crosses
divisions
Table
D.
taken
for cytological
begins
after
commenced
at 30’.
be seen
were
some
hours,
nuclear
had
can
and
Treatment
)--Division
the
supplied
the minimal
while
the
place
beyond
this
ultimately
devoid
48
Frequencies
Cross*
of
Professor
to determine
intervals
growth
next
ascospores
any
GM-0965.
kindly
by
on
25’C.
12 hours,
at
eight
seen in
frequent.
H.
investigation
F945
treatment
not take
but were
growth
produce
strain
cytologically
every
Development
did
protoperithecia
darkened
protoperithecial
the
supplied
temperature
of these
also were
taken
that ot 25” protoperithecial
in
kindly
strains
together
incubated
at
and
of asci
such
and
both
was
from
rapidly
from
15300
crosses
during
NJ.
“multi-spored”.
re-isolated
strain
expand
wave
(crisp),
hours.
Provided
During
be termed
cr
stained
protoperithecia
first
will
G rant,
on sexual
reproduction
in Neurosporo,
of the occurrence
of asci with
more
fixed,
to 30’ and some
It was found
tl further
osci
were
and
P. H.S.
California.
Multi-
of the
24 hours
a U.S.
crasso.
made
by inoculating
Part of each
batch
perithecio
by
Pasadena,
re-isolated&n
crosses
were
and Mitchell.
or
part
A. D.
Neurospora
observed.
30°C.
in
Technology,
Greenhill,
in
The
al-2
and
of
and
Asci
was
G-6174,
Institute
crosses
incubation
in which
at
25”
meiosis
to 30’.
was
pro-
Cytolo-
gical
examination
of
particularly
favourable
presence
of
laggards
segregation
the
was
scattered
late
random
of
the
The
Botany,
Howe,
University
H.B.,
isolates
were
Jr.
and
interesting
Neurospora,
features
is the
of temperature
to become
made
and
a double
25”
to 30’
from
of
homothollic
os (I result
tended
similar
to the previous
reported
above
ore
in the new
terrico1a.
Possibly
chromosome
parent,
Transfers
results
were
The phenomena
II.
shortly
after
transfer
to 30”
of chromosomes
segregating
scattered
from
the
mutant,
were
control
used
made
experiments
under
further
when
cross
the
J.
E.
Page.
would
no
obtained
from
American
induce
conidiating
The
myceliol
however,
four
and
future
have
with
UV
but
for
genetic
made
Collection,
irradiation.
Despite
in
trials.
(Mr.
1963).
ways.
that
8ackus
from
of
(Mycologia
terricola
with
(WSF
conidiation
Several
various
indicated
in
Neuro-
because
a transfer
with
eight-
named
unknown
of standard
however,
using
have
they
5000).
terricolo,
555-562)
o new
which
previously
A number
N.
to date
s:
soil
Neurospora
ho-m,
studies.
with
surmise
Fries;
Wertergoard-Mite
developed
by
was obtained
used to culture
Both
mycelia
might
this
of
WSF
media
marked
methods
and
5000
to
stability
of the
irradiated,
up to fourteen
methods,
further
Page
plated
followed
was
by
CI member
--Department
sorbose
of the
of
plating,
Nat.
growth
might
Sci.
Bacteriology,
of the
vigorously,
Genetics).
parents,
and
restricted
in classifying
or absence
of a modifier
expressions
of SC, as
-
two
was
different
growth,
these
to,
which
two
linked
indicated
made
for
on
but
Research
of
easily
Georgia,
of sc could
be
where
the
Table
gene
I.
pan-l
(5531)
in group
was
instead
in
for
College
mutant
SC (scumbo
the linkoz-testers
5801)
LTIA
future
Teachers
Georgia.
marker
either
one
in the
wild-type
IV seemed
con-
in --N. terriin the normolly
in our experiments
approach
Participancy
recognized
from,
kill,
of ascospores
fruitful
Athens,
os a centromere
detection
distinguishable
treatment
the
cent
plates
conidiation
method,
irradiated.
from
100 per
(fluffy)
mutants
conidia
occurring
the
of
first
harvested
of
port-irradiation
be a more
to
but bucto-peptone
purposes.
In the
previously
for
of
linkage
the
group
111
was
One
phenotype.
to
was
and
testers
progeny.
resembled
the phenotypic
expression
of the tester.
types
on solid
medium,
especially
if scoring
wets early.
to the
in
to
short
produce
and
specifically
of suspensions
then
just
the
yet
Foundation’s
linkage
iotion
scored,
The morphological
incorporated
into
phenotypes
closer
irrad
and
will
prove
University
morphological
crc~ssa.
In crosses
1962)
of these
media,
for irradiation
minutes,
efforts
LT2a
1959,
the
bacto-peptone;
l:li.
to score
for conidiation.
cultures
which
had not been
were
both
(NN
on any
mycelio
involved
times
that
hell;
Frost
view
of the occurrence
of o number
of nonconidiating
N.
crassa.
Any
mutations
to ability
to produce
-have
been
masked
by heterokaryosis.
Consequently,
Hsu, K.S.
A modifier
of the
mutant
scumbo
in Neurospora
gave
more
difficulty
a further
commenced.
Deportment
Wisconsin
prelimina~attempts,
bacto-peptone
from
stock
treatment
attempts
One
fragments,
Summer,
more
for
meioses
Laboratory,
and
true
genetic
results
were
conidiation
therefore
by plating
on
always
obtained
several
reasonably
of mycelial
tried:
media
No
was
and
Various
nonconidial.
as one
around
recombinant,
selected
of ascal
isolated
to promote
The
were
two
in two
differed
reirradiated.
used.
latter
conidiation.
growth
followed
were
method
media
The
fragments,
the mycelia
and
(Perkins
delimited
o wild-type
were
wave
species
studies
therefore
Culture
solidified
employed
cola,
however,
in
conidioting
species,
might
Type
yeast.
was
second
sistently
were
described,
most promise
in
We
mutants
following
Irrodiotion
were
species
having
conidia.
enchonce
Neurospora
gave
heaviest
mycelial
The
were
trait.
peptone
plater
25’;
--Genetics
recently
Neurospora
be unusable
produces
malt,
at
first
Gochenaur
Nonconidiation
species,
organism
nonconidiation
the
Bristol.
of this new
chorocteristic
the
in one
poles
and
chromosome
spores
parent,
(Table
I).
investigation.
spored
techniques
shock,
and
CI conidial
Neurospora
spora
revealed,
to the
material.
oscospore
crosses.
such a cross fixed
irregular
numbers
anaphare
abnormal;
(IS protoperithecial
batch
of
at
chromosomol
Single
used
material
from
preparation,
used
type
The
grew
other
There
was no
The presence
be responsible
for
the
dual