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N.S. F. Grant, California Frost, L.C. spored Such used strains (albino), Some Every ot 48 30’; the undergo in all each observed meiosis at meiosis usually number spores completed the and crosses. & fewer than of spores were also the three in black spores one ascus No. Meiosis effect of temperature 24 on the 25’, crosses I, many asci found in 22 of which several asci only of normal and late the on the and protoperithecia reached. incubated at for 25’ about continuously At 25”, however, fifth day and the from 25’ of aberrant than eight asci black. Asci mis-shopen to 30’ asci did WCIS present spores. observed. Never more The highest with than fewer eight ascospores. asci. of osci:- 8-spored Multi-spored Less than 8-spored I. 8 25 0 0 I-14 7.2 4 7 8 IO 3. l-13 7.0 0 2 IO 13 4. 2-14 8. I I 6 IO 9 5. 4-15 9. I 5 8 13 4 Sample l-4. and Samples in some cr, cross: +, + 0 meioses at 30”. during highest Cross: 5. & Estimated The 23 2. * Samples ceeding 8.0 l-5 frequency of the during 25’ each bused when CT, al-2 (I) on cultures all 25’ and asci the +, al-2 x change to 30” of aberrant asci x occurred: (3) change + at 0 25’C. to 30° (2) and ot clt 30’. 25’, during (4) all change at 30’. 25” (5) at to 30” 25”, at 30’. 25 asci appeared were selected to occur transferred at random. in those from the day. transferred multi-spored aberrant I and steadily grossly Norma intervals continues sixth 3 were medium was observation. hours and Number Average batch Barratt, Eatches of of Westergoard incubated at incubation more contained W. from contained the R. transferred frequency clsclJs:- Range Biology, phenomenon eight spores of development a high all Dr. Catcheside. stage in crosses of any ascospores. of spores per the than stage were late at at commenced However, were wets In addition I- of by G. reproductive rest of the the crosses divisions Table D. taken for cytological begins after commenced at 30’. be seen were some hours, nuclear had can and Treatment )--Division the supplied the minimal while the place beyond this ultimately devoid 48 Frequencies Cross* of Professor to determine intervals growth next ascospores any GM-0965. kindly by on 25’C. 12 hours, at eight seen in frequent. H. investigation F945 treatment not take but were growth produce strain cytologically every Development did protoperithecia darkened protoperithecial the supplied temperature of these also were taken that ot 25” protoperithecial in kindly strains together incubated at and of asci such and both was from rapidly from 15300 crosses during NJ. “multi-spored”. re-isolated strain expand wave (crisp), hours. Provided During be termed cr stained protoperithecia first will G rant, on sexual reproduction in Neurosporo, of the occurrence of asci with more fixed, to 30’ and some It was found tl further osci were and P. H.S. California. Multi- of the 24 hours a U.S. crasso. made by inoculating Part of each batch perithecio by Pasadena, re-isolated&n crosses were and Mitchell. or part A. D. Neurospora observed. 30°C. in Technology, Greenhill, in The al-2 and of and Asci was G-6174, Institute crosses incubation in which at 25” meiosis to 30’. was pro- Cytolo- gical examination of particularly favourable presence of laggards segregation the was scattered late random of the The Botany, Howe, University H.B., isolates were Jr. and interesting Neurospora, features is the of temperature to become made and a double 25” to 30’ from of homothollic os (I result tended similar to the previous reported above ore in the new terrico1a. Possibly chromosome parent, Transfers results were The phenomena II. shortly after transfer to 30” of chromosomes segregating scattered from the mutant, were control used made experiments under further when cross the J. E. Page. would no obtained from American induce conidiating The myceliol however, four and future have with UV but for genetic made Collection, irradiation. Despite in trials. (Mr. 1963). ways. that 8ackus from of (Mycologia terricola with (WSF conidiation Several various indicated in Neuro- because a transfer with eight- named unknown of standard however, using have they 5000). terricolo, 555-562) o new which previously A number N. to date s: soil Neurospora ho-m, studies. with surmise Fries; Wertergoard-Mite developed by was obtained used to culture Both mycelia might this of WSF media marked methods and 5000 to stability of the irradiated, up to fourteen methods, further Page plated followed was by CI member --Department sorbose of the of plating, Nat. growth might Sci. Bacteriology, of the vigorously, Genetics). parents, and restricted in classifying or absence of a modifier expressions of SC, as - two was different growth, these to, which two linked indicated made for on but Research of easily Georgia, of sc could be where the Table gene I. pan-l (5531) in group was instead in for College mutant SC (scumbo the linkoz-testers 5801) LTIA future Teachers Georgia. marker either one in the wild-type IV seemed con- in --N. terriin the normolly in our experiments approach Participancy recognized from, kill, of ascospores fruitful Athens, os a centromere detection distinguishable treatment the cent plates conidiation method, irradiated. from 100 per (fluffy) mutants conidia occurring the of first harvested of port-irradiation be a more to but bucto-peptone purposes. In the previously for of linkage the group 111 was One phenotype. to was and testers progeny. resembled the phenotypic expression of the tester. types on solid medium, especially if scoring wets early. to the in to short produce and specifically of suspensions then just the yet Foundation’s linkage iotion scored, The morphological incorporated into phenotypes closer irrad and will prove University morphological crc~ssa. In crosses 1962) of these media, for irradiation minutes, efforts LT2a 1959, the bacto-peptone; l:li. to score for conidiation. cultures which had not been were both (NN on any mycelio involved times that hell; Frost view of the occurrence of o number of nonconidiating N. crassa. Any mutations to ability to produce -have been masked by heterokaryosis. Consequently, Hsu, K.S. A modifier of the mutant scumbo in Neurospora gave more difficulty a further commenced. Deportment Wisconsin prelimina~attempts, bacto-peptone from stock treatment attempts One fragments, Summer, more for meioses Laboratory, and true genetic results were conidiation therefore by plating on always obtained several reasonably of mycelial tried: media No was and Various nonconidial. as one around recombinant, selected of ascal isolated to promote The were two in two differed reirradiated. used. latter conidiation. growth followed were method media The fragments, the mycelia and (Perkins delimited o wild-type were wave species studies therefore Culture solidified employed cola, however, in conidioting species, might Type yeast. was second sistently were described, most promise in We mutants following Irrodiotion were species having conidia. enchonce Neurospora gave heaviest mycelial The were trait. peptone plater 25’; --Genetics recently Neurospora be unusable produces malt, at first Gochenaur Nonconidiation species, organism nonconidiation the Bristol. of this new chorocteristic the in one poles and chromosome spores parent, (Table I). investigation. spored techniques shock, and CI conidial Neurospora spora revealed, to the material. oscospore crosses. such a cross fixed irregular numbers anaphare abnormal; (IS protoperithecial batch of at chromosomol Single used material from preparation, used type The grew other There was no The presence be responsible for the dual