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AltSplice AltTrans Makes gene-EST alignments. Delineates introns/exons, splice events, splice patterns, peptide variants, etc. Annotates for: Splice signals, SNP, expression states, humanmouse conservation, etc. Passes on the data of splice patterns, confirming ESTs, and alignments to AltTrans Examines the ESTs for polyA cleavage site and signal motif. Groups the ESTs (that could be annotated for the above) based on splice sites and terminating polyA site to form Transcript Patterns. AltPAS Detects polyA sites gene-wide independent of splicing patterns. Integration pipeline Maps AltPAS polyA sites onto AltTrans genes. Consolidates the (AltTrans & AltPAS) polyA sites. Annotates the transcript patterns for skipped polyA sites using the combined list. Integrated AltTrans-AltPAS data AltSplice :splice patterns/events/introns/exons. AltTrans: transcript patterns/polyA events/integrated set of polyA sites. Download of AltTrans data Single-box query (gene symbol/keyword/cross references Advanced Query Page Distributed annotation onto Ensembl system