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Review for BCT Landa, 2012 Six elements needed for life CHNOPS Remember these are in the Carbon (CO2) , water (H2O), nitrogen (N2 and NO2 and NO3) cycles. P an S are small Molecule Two or more ATOMS bonded together O2 and CO2 are molecules as is H2O When two or more DIFFERENT elements are bound together its called a compound. Organic Molecules In non-living things: C and H In living things: C, H , O Macromolecules (Organic) Class name Element Function s Carbohydrate C, H, O Monosaccharides: simple fast energy Polysaccharides: Short term energy storage Examples Structure: Carbs can be single carbon rings (mono.) or chains of many rings linked together (poly.) Lipid C, H, O Long term energy storage Insulation Waterproofing (plants) Mono: sugars (glucose, fructose) Poly: starch, fiber (cellulose) Fats, waxes, oils Protein C, H, O, N, S Speeds up chemical reactions, Fights infection Builds structures Sends chemical messages Enzymes Antibodies Cartilage Hormones NOT a chain of repeated units Does not form polymers Proteins are large chains of amino acids that need to be folded into a specific shape to work. Macromolecules (Organic) Nucleic C, H, O, Stores information Acids N, P on how to make proteins, Makes up our genes (single recipes for a protein) DNA – master copy of all genes RNA – temp copy of single gene Nucleic acids are chains of nucleotides Sequence of nucleotides (ATGC) determines information Enzymes What class of organic compounds? ◦ Proteins Job ◦ Speed up chemical reactions by lowering the amount of energy needed Conditions which affect an enzyme. ◦ Temperature, pH, concentration Enzymes and Structure Enzymes (folded chains of amino acids) must ◦ be folded in a specific way (correct 3D structure) in order for their substrate (the molecule they are breaking down/linking together) can fit together with the enzyme Processes requiring enzymes ALL chemical process in a living thing require enzymes Examples: photosynthesis, digestion, cell respiration, mitosis, meiosis. Replication, transcription, translation….. Homeostasis stable internal environment that is regulated and maintained regardless of what is going on outside the cells/body Three conditions that must be maintained ◦ Temperature, pH, chemical levels (glucose in the blood, sodium, etc) Functions of Water Temperature regulation – sweat Lubrication – joints Transportation of nutrients – blood Senses -in vision/taste/smell/hearing Medium – for reactions Regulating flow in/out of cell Cells regulate the flow of molecules in and out of the cell using the CELL MEMBRANE (PLASMA MEMBRANE). __DIFFUSION____ is the process that moves solutes from high concentration to low concentration. ___OSMOSIS____ is the process that moves water from hypotonic (low solute/high water) areas to hypertonic (high solute/low water) areas ____ACTIVE TRANSPORT______________ is a process that requires energy to move charged ions through the membrane. How does the membrane control flow? Depends on the size, charge and solubility (polar or nonpolar) of the substance. ◦ Small, uncharged and non-polar chemicals dissolve easily (diffusion/passive transport) ◦ Large, charged and polar molecules need help of a transport protein and/or energy (facilitated diffusion or active transport) How are different molecules used? Sugars are used by the ____mitochondria__ for cell respiration Proteins are broken down into subunits called ____amino acids______ and used to build new proteins Cells are made of: many small structures called ___organelles______________ each of which has a specific job. List several jobs performed by the parts of the cells: When do Cells Divide? Growth and reproduction Repair injury Replace dead cells Replication and DNA How do cells ensure that the new cells will have all the same information that the old cells do? ◦ Copy the DNA using DNA replication Identical DNA..but different cells???? If the DNA is identical, how do we get specialized cells? ◦ can use different sets of the genes (within the same DNA to make different proteins. ◦ Analogy: two people with the same cookbook can have different dinner parties just by making different recipes. The process by which cells become specialized is called _differentiation_ Why don’t cells get smaller as they divide? Because they GROW!!!!! After fertilization, what happens to a zygote? ◦ Must begin to divide through mitosis. How do cells differentiate? ◦ They “move around” cells in different chemical environments have different genes activated (turned on). What happens if early cells in the embryo separate? IDENTICAL TWINS Mitosis and Meiosis Mitosis ◦ Happens when we need more body cells ◦ Produces two IDENTICAL cells Meiosis: ◦ Happens when we make reproductive cells ◦ Produces different cells (due to crossing over/genetic recombination) with ½ as many chromosomes as a body cell. # of Chromosomes Body (Somatic) Cells Reproductive (Gamete) Cells ◦ ½ as many chromosomes as body cells ◦ Will fuse with another reproductive cell during fertilization (embryo now has cells that has a complete set (2N) of chromosomes). If mistakes during cell division occur During meiosis, a mistake would lead to a defect in the entire body of an offspring. During mitosis, a single body part will probably be affected Stem cells vs. Regular body cells Stem cells are not specialized (undifferentiated) and have the capacity to differentiate into any type of cell. Body cells are already specialized and can only produce one type of cell Stem cells Mostly in embryonic tissue Some partially differentiated stem cells are found in body parts (ie bone marrow) Potential to cure/prevent disease ◦ if we can learn how to direct stem cells to become specific cell types we might be able to “grow” organs and repair spinal cord injuries. Disrupting Normal Body Functions The structure of the body is necessary to maintain proper function of the body. Damaging cells, organs and organ systems all have a significant impact on how the organism as a whole functions. For each of the following describe how it disrupts the normal functions of the body as well as possible causes. Disruption: Mutation change in the DNA; changes the recipe for the protein; protein may or may not work properly; Example ◦ : defective enzyme = lactose intolerance Pathogens bacteria and viruses; destroy cells or compete with our cells for nutrients in our body; throw off internal homeostasis Drugs/Poisons can alter the effectiveness of proteins in the body and prevent them from doing their job; can disrupt homeostasis Sample Problems Many diseases are caused when the body cannot digest particular molecules in our diet. For example, Lactose intolerance is caused when individuals cannot digest the sugar found in milk. What type of molecule is a person missing if they are lactose intolerant? ◦ ◦ ◦ ◦ Lipid Monosaccharides Nucleic Acids Proteins Problem 2 Which of the following is NOT made of a long chain of repeating units called monomers? ◦ ◦ ◦ ◦ Lipid Carbohydrates Nucleic Acids Proteins Problem 3 The information on how to make _____________________ is stored in the _______________________. ◦ ◦ ◦ ◦ Carbohydrates; Proteins Proteins; Nucleic Acids Nucleic Acids; Proteins Proteins; Carbohydrates Problem 4 The MOST essential inorganic molecule for all living things is _______________. ◦ ◦ ◦ ◦ CO2 Sugars Water Oxygen Problem 5 The human body can make certain amino acids, but has to get others from their diet. Lacking certain amino acids will interfere with your body’s ability to make which of the following? ◦ ◦ ◦ ◦ Lipid Carbohydrates Nucleic Acids Proteins Problem 6 The body can extract energy from several different types of molecules. Which one of the following provides SIMPLE FAST energy? ◦ ◦ ◦ ◦ a. Monosaccharides b. Lipids c. Polysaccharides d. Proteins Problem 7 Which of the following best explains why enzymes are necessary for many cellular reactions? ◦ A. Enzymes supply the oxygen necessary for the reactions. ◦ B. Enzymes change reactants from solid to liquid during the reactions. ◦ C. The reactions take up too much space in the cell if enzymes are missing. ◦ D. The reactions are too slow to meet the needs of the cell if enzymes are missing Problem 8. Michelle is working in a lab. She is trying to use an enzyme to digest starch into single monosaccharides. She adds starch to a tube and then adds the enzyme. She knows that heat helps chemical reactions so she places the tube on a hot plate and waits for a reaction. After 10 minutes nothing has happened. What went wrong? ◦ ◦ ◦ ◦ Michelle added the wrong enzyme Starch cannot be broken down into monosaccharides The heat probably denatured the enzyme 10 minutes is too short, you need to wait for hours for enzymes to work Problem 9. If an animal cell is placed in distilled water it may swell and burst. The bursting of the cell is a result of which process? ◦ ◦ ◦ ◦ Enzyme activity Active transport Osmosis Respiration Problem 10. Joe is studying different types of cells. He notices cells all have a unique shape and sometimes even have special structures that aren’t found in other cells. Which of the following would be the best conclusion for Joe to make about his observations? a. Some of the cells he is examining are mutated b. Different cell types are specially adapted to work for specific organisms c. Every different species has a different type of cell. d. He must be looking at prokaryotic and eukaryotic cells. Problem 11. The body as well as individual cells, is in charge of keeping homeostasis. Which of the following could be the result of the body failing to regulate a stable internal environment? ◦ ◦ ◦ ◦ Diabetes Dehydration Fever All of the above Problem 12. Which two processes must happen before a cell goes through mitosis? ◦ The DNA must replicate and the chromosomes must separate ◦ Two cells must form and the cells must grow ◦ Cells must grow and DNA must replicate ◦ Cells must divide and the DNA must replicate Problem 13. DNA is folded into ___________ during mitosis and then unraveled again at the end. ◦ ◦ ◦ ◦ Proteins Chromosomes The nucleus RNA Problem 14. Which of the following can happen if cell division is not regulated properly? ◦ ◦ ◦ ◦ Diabetes Cystic fibrosis Cancer Influenza Problem 15. Normal cell division is involved in all of the following EXCEPT: ◦ ◦ ◦ ◦ Repair of an injury Growing Making reproductive cells Protein synthesis Problem 16. Neurons, cells in the nervous system, and skin cells are very different. They have a different shape, different function and are even found in different places in the body. BUT upon close inspection, neurons and skin cells have the exact same set of DNA. What causes all the differences? ◦ In reality their DNA is different it only looks the same. ◦ They have the same DNA but access different genes to make different proteins. ◦ Neurons don’t use their DNA ◦ Skin cells are actually dead cells. Problem 17. Place the following stages in the order that they happen: a. Fertilization mitosis differentiation organ formation b. Fertilization differentiation mitosis organ formation c. Mitosis organ formation differentiation fertilization d. Fertilization differentiation organ formation mitosis. Problem 18. The body cell of a carrot has 18 chromosomes. That means that the carrot’s reproductive cells will each have _________ chromosomes and a carrot zygote will have _________________ chromosomes. a. 18; 18 b. 18; 36 c. 9; 9 d. 9; 18 Problem 19. A doctor performs amniocentesis on an embryo to find that it contains an extra chromosome. Which is a possible diagnosis for the baby? ◦ ◦ ◦ ◦ Cancer Down Syndrome Influenza Color blindness Problem 20 A young patient is diagnosed with the genetic disorder, Lactose Intolerance, which results in the inability to digests milk products due to a missing enzyme called lactase. What is most likely the cause of lactose intolerance in this patient? a. The patient is allergic to milk. b. The patient stopped consuming milk products. c. A disease destroyed the lining of the digestive tract. d. A mutation occurred in the gene that is responsible for producing lactase. Problem 21. Which of the following diseases/disorders is contagious? a. Cancer b. Strep throat c. Color blindness d. Down syndrome Problem 22. Which of the following diseases/disorders is heritable (inherited)? ◦ ◦ ◦ ◦ Cancer Strep throat Color blindness AIDS Problem 23. Disease, in general, can basically be defined as: a. When our bodies are infected with foreign pathogens. b. When mutations disrupt our ability to make proteins. c. When organs are not formed properly d. When an imbalance is created that prevents our body from functioning normally. MATTER AND ENERGY TRANSFER The atoms we are built out of cycle through the ecosystem constantly. Describe in words or labeled drawings how each of the following atoms/molecules cycles through our ecosystems: Pictures of each of these can be found in the book. Understand CONCEPTS don’t worry about specific chemical changes Cycles of matter (C, N, H, O) Carbon Nitrogen Water Once in an organism, what are these atoms used for? ◦ To build new cells Moving matter and energy How are these atoms passed from one organism to the next? ◦ When we eat a plant the atoms that make up the plant get digested and then our body uses them to build new cells for us. How can these atoms be returned to the non-living parts of our ecosystem? ◦ When we die all our atoms are returned to the soil through decomposition or released into the atmosphere (CO2) Can we make new matter NO….NO…..NO We just recycle the matter that is here. Living things today are made of the exact same atoms that living things were made of back in dinosaur times What about energy? The initial source of all energy is the SUN How is this true even for strict carnivores? ◦ The sun provides energy for the plants to photosynthesize. Herbivores eat the plant and energy gets transferred to them. Then the carnivores eat the other animals and the energy is transferred to them. The energy pyramid The shape of the pyramid implies there is “less” at the top. “Less” what and why is this necessary for success of the ecosystem? Less energy and less biomass (atoms!) WHY?: because it is the lower levels that feed and support the upper levels. Need to have more food than eaters or else you run out. What does a food web demonstrate? Flow of energy and matter from the producers through the apex consumers Impact of removal of a single species: Species that depend on the removed species (predator) for food will decline in population Species that the removed species consumes (prey) will become over populated causing a shortage of their (the prey’s) food source. Amt of energy passed from one trophic level to the next 10% is passed from one level to the next What happens to the rest? ◦ Ultimately becomes thermal energy (heat) Biologically, how do we extract energy from food? Cellular respiration: ◦ Key enzymes in the cell along with the mitochondria break the glucose down into CO2 and Water and transfer the energy to ATP molecules. ◦ Other foods, like protein and fats are first converted into types of sugars that can go through the same cellular respiration process. Diagram of PS and Respiration Three forms of energy in an ecosystem Light – sun Chemical – glucose Thermal – body heat released after using energy Two purposes of food: Energy (we make ATP) Building materials ◦ We use atoms and rearrange them to make cells for “us”. ◦ (also specific nutrients that are used directly like vitamins, amino acids, minerals) Why does an organism NOT disintegrate when it is alive? A constant supply of energy keeps our body maintained When we die the energy supply ceases and body processes no longer are maintained. Interdependence of 2 or more Organisms Symbiosis ◦ Three forms Mutualism Commensalism Parasitism Problem 1. In an experiment, chickens were fed grain that contained a chemical marker in its proteins. The presence of the marker can be detected in organisms. Which of the following is the most reasonable prediction from this experiment? a. The marker will only be found in the grain. b. Both chickens and wolves will have the marker c. Wolves will have the marker, but chickens will not. d. The marker will only be found in the animals’ wastes. Problem 2 What two things are passed from one organism to another in the food chain? ◦ ◦ ◦ ◦ Energy and Heat Matter and Atoms Energy and Matter Water and food Problem 3 Which types of organisms assist in cycling atoms back into the soil? ◦ ◦ ◦ ◦ Producers Primary consumers Apex consumers Decomposers Problem 4 . Which hypothesis would most likely be tested using this setup? A. Green water plants release a gas in the presence of light. B. Roots of water plants absorb minerals in the absence of light C. Green plants need light for cell division. D. Plants grow best in the absence of light. Problem 5 At position Y, carbon is most likely to be in which of the following forms? A. protein B. carbon solid C. carbohydrate D. carbon dioxide Problem 6 Which of the following diagrams correctly represents an energy pyramid from this web? B Part C Interdependence Define the following terms in relationship to their position in the hierarchy of ecosystems Populations: all of one single species that lives in a region Communities: all of the living things in a region (multiple species combined) Ecosystems: All of the living things AND the non-living things they depend on Biomes: All the ecosystems at a particular climate Biospheres: The entire region of the planet in/on which life can exist Food Webs Explain how each organism in a food web can be dependent on eachother. They can depend on each other for food, reproduction, habitat selection; no organism can survive entirely on its own. What does the term symbiosis mean? Relationship between two organisms that is necessary for the survival of one or both the species. THREE TYPES Mutualism – win/win Commensalism – win/no effect Parasitism – win/lose Human Impact on Environment List five activities and explain HOW ◦ Excessive use of fossil fuels – build up of CO2; traps extra heat; global warming ◦ Over developing wooded areas – destroys habitats of organisms; impacts the food chain ◦ Deforestation – removes trees which reducing oxygen production and CO2 removal ◦ Excessive use of chemicals like CFC’s – depletion of the ozone layer; more harmful UV rays hitting earth ◦ Pollution of lakes and rivers – damages water life; contaminates water supply When left alone (naturally) How does an ecosystem balance itself to keep all the organisms at the top of the food chain from wiping out all the organisms below? ◦ As a consumer increases the food supply of that consumer decreases; eventually there will not be enough food and the consumers will die off; As the consumers die, the food supply is rebuilt and so on Problem 1 1. Which of the following is most likely to lead to an increase in the number of foxes over time? A. a decrease in owls B. an increase in hawks C. an increase in mountain lions D. a decrease in raspberry bushes Problem 2 If many trees are removed from a forest, what is the most immediate effect on the carbon cycle in that forest? A. increased rates of decomposition B. decreased use of atmospheric CO2 C. decreased combustion of fossil fuels D. increased production of organic compounds Heredity and Reproduction Organisms reproduce, develop, and have predictable life cycles. Organisms contain genetic information that influences their traits, and they pass this on to their offspring during reproduction. Heredity and Reproduction 1. Genes are segments of DNA molecules located in the chromosome of each cell. DNA molecules contain information that determines a sequence of amino acids, which result in specific proteins. 2. Inserting, deleting, or substituting DNA segments can alter the genetic code 3. An altered gene may be passed on to every cell that develops from it. The resulting features may help, harm, or have little or no effect on the offspring’s success in its environment. 4. Sorting and recombination of genes in sexual reproduction result in a great variety of possible gene combinations in the offspring of any two parents Basic Information According to the central dogma of all living things: _DNA__contains the master copy of all our information. This information is broken up into sections, or recipes, called ___genes__. Each gene contains the directions to make a single __protein_____ by linking together the proper sequence of _amino acids__. Before building a protein, a temporary copy of the single recipe must be made. This copy is called __RNA_. Ultimately it is the __protein_ that gives you the traits that we have and the __genes/DNA_ that teaches us how to make them. DNA Kept in the NUCLEUS Wound up DNA: ◦ Chromosomes Mutation Change in the DNA What problems does it cause? ◦ Since the DNA contains the directions to make proteins, changing the DNA could change the protein; Can cause diseases, can have no effect or can improve the function of a protein; What types of changes are considered mutations? Insertions – add a nucleotide; Deletions – remove a nucleotide; Substitution – swap one nucleotide for another ANY CHANGE IN DNA IS A MUTATION! Effect of a mutation on future cells Since all the DNA is copied before cell division, a mutation in the parent cell will be copied and passed to the new cells. Mutations do not have to be negative. They can be helpful or have no effect. What is genetic variation and why is it important? Genetic variation is diversity within or between species because of their DNA differences. IMPORTANCE: It is necessary to have variation so that natural selection can occur. Differences give individuals different ability to survive and reproduce. This allows for evolution Three things that increase genetic variation in a population Mutation Sexual reproduction – mixing the DNA of two individuals Recombination/crossing over – mixing up your own DNA to make various different reproductive cell Migration – introduction of new alleles into a population from a different population Genetic Variation Which process listed above also explains how one set of parents can seemingly have an infinite number of different children? Recombination Problem 1 Fireflies produce light inside their bodies. The enzyme luciferase is involved in the reaction that produces the light. Scientists have isolated the luciferase gene. A scientist inserts the luciferase gene into the DNA of cells from another organism. If these cells produce light, the scientist knows that which of the following occurred? A. The luciferase gene mutated inside the cells. B. The luciferase gene was transcribed and translated. C. The luciferase gene destroyed the original genes of the cells. D. The luciferase gene moved from the nucleus to the endoplasmic reticulum Problem 2 Which of the following best describes the result of a mutation in an organism's DNA? ◦ ◦ ◦ ◦ A. The mutation may produce a zygote. B. The mutation may cause phenotypic change. C. The mutation causes damage when it occurs. D. The mutation creates entirely new organisms. Problem 3 The diagram shows the positions of the genes for flower color and stem length in a pea plant. For these two genes, what is the maximum number of different allele combinations that can be formed normally in gametes produced from this cell? A. 2 B. 4 C. 6 D. 8 PT Pt pT pt Problem 4 If the DNA is mutated which of the following statements is true? ◦ ALL other cells in the body will have the same mutation. ◦ All proteins in the cell will be mutated ◦ Any proteins, RNA or cells that come from the mutated DNA will have the mutation ◦ The cell will fix the mutation and nothing will happen. Problem 5 Which of the following populations is likely to have a high degree of genetic diversity? ◦ An asexual population of bacteria with a very low mutation rate (asexual = NO VARIATION) ◦ Sexually reproducing species where crossing over is frequent ◦ A population of sheep created by cloning (NO VARIATION) ◦ A very small population of sexually reproducing species. (SMALL POPULATIONS HAVE LOW NUMBER OF VARIATIONS) EVOLUTION AND DIVERSITY Sometimes, differences between organisms of the same kind provide advantages for surviving and reproducing in different environments. These selective differences may lead to dramatic changes in characteristics of organisms in a population over extremely long periods of time. KEY LEARNINGS 1 New traits may result from new combinations of existing genes or from mutations of genes in reproductive cells within a population 2. Molecular evidence (e.g., DNA, protein structures, etc.) substantiates the anatomical evidence for evolution and provides additional detail about the sequence in which various lines of descent branched 3. The principles of evolution (including natural selection and common descent) provide a scientific explanation for the history of life on Earth as evidenced in the fossil record and in the similarities that exist within the diversity of existing organisms Key Learnings 4. Evolution occurs as a result of a combination of the following factors: A. Ability of a species to reproduce B. Genetic variability of offspring due to mutation and recombination of genes C. Finite supply of the resources required for life D. Natural selection, due to environmental pressure, of those organisms better able to survive and leave offspring Evolution is defined as: How a population changes over long periods of time What key scientist is credited with the bulk of our understanding about evolution? ◦ DARWIN Physical or molecular features used to support evolution and shared common ancestors: DNA/Protein structures (molecular) – more similar the sequences or structure the more closely related two organisms are Anatomy – similar body structures can provide information about relationships and ancestry Embryonic development – the stages we go through during development can be informative Fossils – can tell us approximately how long ago an organism lived How does natural selection work? Requires presence of variation! Environmental stress (limited food and/or other resources) must be present Competition for resources occurs - and because they are different some are better at competing that others Survival of the fittest – the organisms best adapted win the competition and increase their chances of surviving and reproducing Descent with modification – winners reproduce more and the next generation consists of more individual’s with the “winner’s” traits that the “loser’s” traits. How does genetic variation promote evolution? Allows organisms to be on different playing fields as far as competition goes. Increases the chances that there is a “super” trait out there than will give the organism a survival advantage Does evolution = new species? No. EVOLUTION = a shift in the frequency of certain alleles (variations of genes) or traits A population does NOT have to become a separate new species (or split and become two species) in order for it to be “evolving”. Problem 1 Thousands of years ago, giraffes with short necks were common within giraffe populations. Nearly all giraffe populations today have long necks. This difference could be due to: A. giraffes stretching their necks to keep their heads out of reach of predators B. giraffes stretching their necks so they could reach food higher in the trees C. a mutation in genetic material controlling neck size occurring in some skin cells of a giraffe D. a mutation in genetic material controlling neck size occurring in the reproductive cells of a giraffe. Problem 2 Scientists have concluded that snakes evolved from an ancestor with legs. Which of the following statements provides the best evidence for this conclusion? A. Most species of snakes live on land. B. Snakes move extremely fast to catch their prey. C. Snakes have a well-developed backbone and muscular system. D. Some species of snakes have limb buds during their embryonic development ◦ The limb buds indicate that ancestors may have had limbs Problem 3 Which of the following best explains how the fossil record provides evidence that evolution has occurred? (need to look for an answer that talks about change over time = def of evolution) ◦ A. It indicates that forms of life existed on Earth at least 3.5 billion years ago. ◦ B. It indicates the exact cause of structural and behavioral adaptations of organisms. ◦ C. It shows how the embryos of many different vertebrate species are very similar. ◦ D. It shows that the form and structure of groups of organisms have changed over time Problem 4 On island chains like the one shown above, animal populations that spread from the main island to the other islands can evolve into separate species. CHOOSE THE STATEMENT ON THE NEXT PAGE THAT best explains what favors speciation in these situations? Problem 4 (cont) ◦ A. Predators on the main island can easily migrate to follow the populations to the other islands. ◦ B. Lack of disease on the other islands enables the populations to grow and change without limit ◦ C. The physical separation of the islands limits gene flow and interbreeding between the populations. ◦ D. The climatic conditions of the islands allow the populations to breed all year and produce several generations WE ARE FINISHED! Problem 1 answers: Problem 6