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OPEN UNIVERSITY LIBRARY Title: PROGRAMME SEQUENCE LIST Science course unit 15. Producer: J i r n StevensonContributors: M . J . Pentz y Stephen Hurry> Steven Rose. Summary: s 100/15 (1972). Tape No. 6HT/70550Project No. 00521/2190. O.U. film no. Date Recorded. 10.2.1972 1st TX. 23.4.1972. Form VTR. Duration 24'03'V 591.8764 Class nos. 574.19254 cu Part 1: Some of the techniques of the salivary amylase home experiment are demonstrated. Part 2: The metabolism of glucose in isolated slices of rat liver is followed. m Seq. T i me. 1 • 02" Footage. 16 Mike Pentz introduces the programme. He points out that biological processes are chemical• 78 Stephen Hurry, with part of the S 1OO home experiment kit, goes through some of the procedures in the salivary amylase experiment which students may have had trouble with. He starts by running various solutions through the colorimeter to arrive at a standard curve. This is plotted on a large graph. 87 Hurry next demonstrates how a saliva sample is collected and prepared for the main part of the experiment. 1 5 * 20" 1| 5-55" Sequence List. 6«5O" 100 Hurry plots the values which were obtained from a salivary amylase experiment conducted off camera. 7'28" 108 Mike Pentz introduces Steven Rose. 126 For part 2 of the programme, Steven Rose will demonstrate techniques for studying reaction sequences of glycolysis and glucose oxidation. He begins by drawing the metabolic pathway by which glucose is converted during glycolysis to pyruvate which goes to C O 2 and lactate. Rose explains why a radio-active trace would be added to the glucose for a experiment. 143 Rose dissects out some liver tissue from a rat and prepares it for the experiment. 8«49" 1O'O7" Rose, with a Warburg manometer, explains how the apparatus works. He then explains how the COg produced in the experiment is absorbed. Sound Cue ....1 use it. By the way..• S 1OO/15 ( 1972) . . . 2. . . PROGRAMME SEQUENCE LIST OPEN UNIVERSITY LIBRARY Continuation Time 13 • OO" 15'2O" l8«42" 23 I 27" Sequence List Footage 180 Rose begins the experiment to measure oxygen uptake in the liver tissue. 2O8 Rose examines three manometers which had been incubating for some time. He then stops the reactions by destroying the enzymes with a dilute acid. 248 Rose explains and then demonstrates the use of a scintillation counter for measuring COj. puoduction from the reaction. 3O1 Rose explains and demonstrates a technique for analysing lactic acid production enzymically. He uses a graph which has plotted the absorbent spectra of NAD and NADHz. obtained from a spectra photometer. Rose looks at a curve which was obtained for a run lasting about ^ hour. Rose sums up. 24•O3" 3O8 Credits. Sound Cue ...of the tissue. Let's come back...