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TORTORA • FUNKE • CASE Microbiology AN INTRODUCTION EIGHTH EDITION Chapter 5, part C Microbial Metabolism Feeding methods Feeding methods Chemotrophs • Use energy from chemicals. – Chemoheterotroph Glucose NAD+ ETC Pyruvic acid NADH • Energy is used in anabolism. ADP + P ATP Chemotrophs • Use energy from chemicals. – Chemoautotroph, Thiobacillus ferroxidans 2Fe2+ NAD+ ETC 2Fe3+ NADH ADP + P ATP 2 H+ • Energy used in the Calvin-Benson cycle to fix CO2. Phototrophs • Use light energy. Chlorophyll ETC Chlorophyll oxidized ADP + P ATP • Photoautotrophs use light energy in the CalvinBenson cycle to fix CO2. • Photoheterotrophs use light energy and organic compounds. Metabolic Diversity Among Organisms Nutritional type Energy source Carbon source Example Photoautotroph Light CO2 Oxygenic: Cyanobacteria plants. Anoxygenic: Green, purple bacteria. Green, purple nonsulfur bacteria. Photoheterotroph Light Organic compounds Chemoautotroph Chemical CO2 Iron-oxidizing bacteria. Chemoheterotroph Chemical Organic compounds Fermentative bacteria. Animals, protozoa, fungi, bacteria. Metabolic Pathways of Energy Use • Polysaccharide Biosynthesis Figure 5.28 Metabolic Pathways of Energy Use • Lipid Biosynthesis Figure 5.29 Metabolic Pathways of Energy Use • Amino Acid and Protein Biosynthesis Figure 5.30a Metabolic Pathways of Energy Use • Amino Acid and Protein Biosynthesis Figure 5.30b Metabolic Pathways of Energy Use • Purine and Pyrimidine Biosynthesi s Figure 5.31 Amphibolic pathways • Are metabolic pathways that have both catabolic and anabolic functions. Figure 5.32.1 Amphibolic pathways Figure 5.32.2