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Alternatives to Pesticides • Pheromone pest management • Attract-and-kill systems • Host plant resistance • Cultural control techniques Pheromone Pest Management Pheromone - A chemical substance secreted by an organism that affects the behavior of other organisms of the same species. Sex pheromones - Usually produced by females to attract males for mating, but they may also be produced by males to attract females Aggregation pheromones – Is responsible for the aggregation / congregation of insects at food sites or reproductive habitats. They are very common in bark beetles Trail-marking pheromones – These chemicals are produced by ants and termites which allow other members of the colony to follow or locate their position Uses of pheromones in pest management 1) To monitor populations of insect pests 2) They are used in attract-kill-programs 3) To prevent mating in orchards How does mating disruption works? Washington State Univ Commercially available disruption techniques 2) Aerosol emitters 1) Hand-applied dispensers Rate: 1-2 / acre Rate: 200-500 / acre 3) Sprayable microencapsulated formulations Photo credit: ISCA Tech. Stelinski 2007 Greatly magnified Mating disruption Advantages Specific Long Lasting Difficult for insects to develop resistance Non-toxic Disadvantages Mostly effective for low to moderate pest populations Can be costly Does not kill pest (immigration) Not a stand alone control method Methods to evaluate pheromone efficacy Rubber septum release device Baited sticky trap Trap-shut down Fruit injury counts Counting of pupal skins Dissecting females to determine whether or not they have mated Stelinski 2007 Mechanisms in mating disruption unresponsive receptors on antennae Sensory fatigue habituation in central nervous system False trail-following – male moths follow synthetic pheromone plume as opposed to the plume from a female moth Kairomones - chemical substances produced by one species and and received by a second species. - The chemical substance is beneficial only to the receiver. These chemical substances include attractants, excitants, and stimulants Advantage Promote host finding, oviposition and feeding Attract-and-Kill Systems A pest control device consisting mainly of an attractant and a toxicant Kairomone baited trap LastCallGRB® Newly applied versus 6 wk Contained 0.16% of the GRB pheromone and 6% Pyrocide 3 droplets per vine Experimental trial - Attract & kill • Malathion • GF 120 • SpinTor • Control Effects of conventional and reduced-risk insecticides on A. suspensa Laboratory Experiments a a a b + NuLure P = 0.0062 Captures of GRB in Florida Vineyards Host plant resistance Resistant cultivars: insects may avoid plants for a variety of reasons: Genetic Control Allelochemic nonpreference Diabrotica spp. avoiding cultivars that Non-preference lacks cucurbitacins (Antixenosis) Morphological non-preference Corn earworm, Helicoverpa zea avoid ovipositing in cotton that lacks hair Antibiosis – Plant characteristics that impairs an insect’s metabolic processes. Example pea cultivars with low amino acid levels (nutritional deficiencies) shows resistance to pea aphids Tolerance – The plant has the ability to give satisfactory yields in spite of fairly high injury levels Cultural techniques The use of mulches (reflective and living mulch) Population of natural enemies in cucurbits treated with different mulches Cultural techniques Managing field moisture/irrigation Population of twospotted spider mites in strawberries Soil Moisture levels Cultural techniques • Trap crops • Adjust planting dates • Clean cultivation • Pruning