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LECTURE PHOTOSYNTHESIS DR AKM SHAFIQUL ISLAM ELECTRON TRANSPORT PATHWAY • Occurs within the inner mitochondrial membrane • Electrons are removed from NADH and shuttled through a series of electron acceptors – Energy is removed from the electrons with each transfer • This energy is used to make ATP – NADH 3 ATP – FADH2 2 ATP – O2 is the terminal electron acceptor • ½O2 + 2H+ + 2e- H2O – Anaerobic respiration utilizes a molecule other than O2 as the terminal electron acceptor • e.g., NO3-, SO42-, CO2, etc. • Respiratory chain reaction NADH + H+ +1/2 O2 + 3 ADP + 3Pi NAD+ + 4 H2O + 3 ATP and FADH2 + ½ O2 + 2 ADP + 2Pi FAD + 3 H2O + 2 ATP Overall reaction Glucose + 6 O2 6 CO2 + 6 H2O DGo’= -696 kcal/mol Review of Cellular Respiration – one 6-C glucose oxidized to 6 CO2 molecules – 2 ATP from substrate level phosphorylation in glycolysis – 2 ATP from substrate level phosphorylation in citric acid cycle – Each NADH generates a maximum of 3 ATP • 10 NADH = maximum of 30 ATP – Each FADH2 generates a 2 ATP • 2 FADH2 = 4 ATP – TOTAL possible ATP = 38 Photophosphorylation • Only occurs in photosynthetic cells which contain light trapping pigments such as chlorophyll • Light causes chlorophyll to give up electrons • Energy released from the transfer of electrons (oxidation) of chlorophyll through a system of carrier molecules is used to generate ATP Photosynthesis • Photosynthesis is largely reverse of respiration • Energy in the form of light is captured and used for conversion of carbon dioxide to glucose and its polymers • Photosynthesis is the prime supplier of energy for biosphere • 6 CO2 + 6 H2O + light C6H12O6 + O2 PHOTOSYNTHESIS Who does photosynthesis? • Though plants can photosynthesize, microorganisms are responsible for the majority of the photosynthesis occurring on the planet CHLOROPLASTS • Chloroplasts are the site of photosynthesis – photosynthetic bacteria don’t have chloroplasts, they essentially are chloroplasts • Chloroplasts contain the pigment chlorophyll – Pigments absorb light – Multiple similar forms of chlorophyll exist – The light absorbed is ultimately used to reduce CO2 to glucose • In procaryctes, (cyanobacteria, green sulfur bacteria, purple sulfur bacteria) photosynthesis occurs in stacked membranes • While organelle called the chloroplast conducts photosynthesis for eucaryotes (algae, plants) • Both systems contain chlorophylls which strongly absorb visible light • Two different light-harvesting and reaction system. 1. Photosystem I – activated about 430 nm. 2. While phosphosystem II activated by light with wavelength below 680 nm. In the case of phosphosystem I, the electron used to reduce NADP+. Excited electron from phosphsystem II are passed to phosphosystem I with ADP phosphorylation accomplished once for every excited electron pair transported. Thus the overall stoichiometry for the photosynthetic eucaryotes is H2 O + 4hv + NADP+ + ADP + Pi NADPH + H+ +1/2 O2 + (ATP + H2O) Additional ATPs may be generated from absorbed light energy via cyclic photophosphorylation • Additional ATPs may be generated from absorbed light energy via cyclic photophosphorylation. Electron excited from P700 to P430 flow to cyt b563 and back to P700, causing ADP phosphorylation in the process. 3hv + ADP + Pi ATP + H2O Biosynthesis • The phenomenon that characterize the microbial process are – Substrate or nutrient utilization – Cell growth – Product release Biosynthesis influences all the three • Nutrient requirements are directed by cells need for precursor molecule, stored chemical energy and reducing power • The rate of cell growth is determined by the rate of biosysnthesis, the rate at which new cell materials are formed • Cell growth rate – varies widely. E. coli bacteria can double in 20 min. Synthesis of Small Molecules • Monomeric building blocks are constructed. • Approximately 70 different compounds are required in this purpose – – – – – 4 ribonucleotides 4 hydroxyribonucleotides 20 amino acid about 15 monosaccharides about 20 fatty acids and lipids • ATP, NAD, other carrier and coenzymes involved in the synthesis • Living cell assimilate nitrogen by incorporating it into the amino acids glutamic acid and glutamine First, glutamic acid is formed by reaction between ammonia and a-ketoglutamic acid, one of the TCA cycle intermediate HOOC(CH2)2COCOOH + NH4+ + NADH glutamate dehydrogenase HOOC(CH2)2CHNH2COOH + NAD+ + H2O Additional ammonia can be accepted by adding it to glutamic acid to give glutamine + glutamine synthase HOOC(CH2)2CHNH2COOH + NH4 + ATP HOOCNH2CH(CH2)2CONH2 + ADP + Pi + H+ The second reaction require metabolic energy and ammonia. In some bacteria direct amination of pyruvate to alanine occurs with consumption of NADH • Other amino acids are formed by conversion of glutamate or by transfer of its amino group to other carbon skeletons – Example, glutamate is converted to poline in a sequence of two enzyme-catalyzed reaction plus a spontaneous hydrolysis step C5NH8O4- + ATP + 2(NADP + H+) C5NH8O2- + ADP + Pi + 2NADP+ + H2O • Transpotation from glutamate to alanine and aspartate Glutamate + oxaloacetate a-ketoglutarate + aspartate Glutamate + pyruvate a-ketoglutarate + alanine