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BIOCHEMISTRY the chemistry of life Chpt. 3 Matter, energy & organization 1. Water 2. Carbon compounds 3. Molecules of life WATER The molecules that make you you are NOT water… (Proteins, Carbohydrates, Lipids & Nucleic Acids) why then is water important for life? Why will you die w/out it? • Somehow, it has properties that other molecules don’t… properties that are vital for life. • But what is it about a water molecule that makes it so special??? • LET’s FIND OUT TODAY!!!! Water molecule? Or… A: WATER IS A POLAR MOLECULE… it has a partial positive & partial negative side to it… call them “poles”. This gives it some unique properties. Polarity means separation of + & - charges • Oxygen is a more electronegative element than hydrogen- it’s pull on the electrons is greater. • The shared electrons spend more time around the OXYGEN than they do around the HYDROGENS. Water is formed via • OXYGEN is partially polar covalent bonds between the hydrogen • HYDROGENS are partially + and oxygen atoms. Oxygen gas Is O=O This happens when one atom is more electronegative than another in the covalent bond. Because of it’s polarity… water is a great solvent! Polar substances will readily dissolve in water. • Sugars • Proteins • Ionic compounds ex. Table salt (Na+Cl-) Watch the salt disappear… IT’S MAGIC!!! No matter what the charge is on the solute, it can be completely surrounded by water molecules. The partially negative OXYGEN is attracted to the positive ion OR the partially positive HYDROGENS are attracted to the - Cl Salt crystals dissolve in water when Na+ and Cl- ions are separated and surrounded by water The polarity of water makes hydrogen bonding possible • A HYDROGEN Bond extends from the + hydrogen atom in one molecule to the partially negative atom in another molecule (N, O, S). • N (nitrogen) O (oxygen) and S (sulfur) are all electroneg. • Shown as a dotted line. Hydrogen bonds are weak bonds that hold water molecules together or to other charged substances. This results in COHESION between water molecules. (water-water H bonds) • Jesus lizard • Water Strider The high surface tension of water allows for these two miracles. Increased habitat for organisms. Water resists being pulled apart from other water molecules. Surface tension is molecules resisting being pulled apart because of their hydrogen bonds! BIOLOGICAL MOLECULES LIKE DNA ARE HELD TOGETHER BECAUSE OF HYDROGEN BONDS ADHESION attractive force between unlike polar substances Adhesion of water molecules to the xylem in these Giant Sequoia trees lets it rise 275 ft.by capillary action. Capillarity or capillary action is the rising of water up small tubes w/out the use of Energy (this happens in XYLEM tubes). TRANSPIRATION • When water climbs through Xylem tubes in plants, from soil to the roots to the leaves to the atmosphere this is called transpiration. • A continuous water chain is “yanked” up w/ each evaporated water molecule from the leaf. • This is called the COHESIONTENSION MODEL and it explains how plants transport water and minerals without using any ATP/energy. BECAUSE OF IT’S HYDROGEN BONDS, WATER IS A GREAT TEMPERATURE MODERATOR Where would you like to be on a hot day? Water absorbs energy into it’s HYDROGEN BONDS before it starts gaining energy itself. Important for life/cells which are 90% water. Helps to maintain homeostasis. Water is excellent at preventing temperature swings (getting too hot or too cold under normal circumstances)because it has an extremely high SPECIFIC HEAT. =‘s the amount of energy needed to change 1 g of the substance by 1 degree celcius. WATER BOILS AT A HIGHER TEMPERATURE THAN ALCOHOL. WHY? Water is MORE polar & has more hydrogen bonds. Because of hydrogen bonding WATER IS LESS DENSE AS A SOLID… • Hydrogen bonding between water molecules at low energy levels places them farther apart than as a liquid or gas. • This means ice (solid water) floats. • An important reality for aquatic organisms. RECAP OF THE PROPERTIES OF WATER THAT MAKE IT ESSENTIAL FOR LIFE 1. Polarity: good solvent. 2. Hydrogen Bonding: surface tension, cohesion, adhesion, capillarity. 3. Hydrogen Bonding: temperature moderation & expands upon freezing. THE WATER LAB 1. Magic Salt Disappearing Trick 2. Drops on a Penny 3. 4. Sprinkle a spoon-full of salt into a beaker/glass of water. Stir it around and make it “disappear”. Where did it go? How do you explain this using your knowledge of water chemistry? Place as many drops of water on the face of a penny as you can without them spilling over the side. Explain how you can make this snow-globe shaped dome at the top! Can you Break these Bonds Take two flat pieces of glass (microscope slides work well) and hold them flat sides together. Pull them apart. Now put a film of water between them and try to pull them apart. Can you? Explain what’s going on. Rainbow Tree Simulation Take a piece of chromatography paper and use a vis-à-vis (water soluble ink) marker to draw a line near the bottom of the strip that is horizontal to the paper. Put the paper in a shallow cup of water with the end of the paper w/ the line in the water. The water should be lower than the drawn line. Watch as water creeps up the paper and see what it does to the ink!!!! Explain this in terms of cohesion and adhesion. Matter, energy & organization 1. Water 2. Carbon compounds 3. Molecules of life II. CARBON COMPOUNDS ORGANIC COMPOUNDS are: • Molecules formed by the actions of living things. • Molecules containing CARBON & HYDROGEN. • Molecules with a carbon backbone-carbon atoms covalently bonded to other carbon atoms & to other elements as well. • The other clusters of atoms attached to the carbon backbone are called functional groups. • Ex. Alcohol functional group Organic molecules must be made of carbon. Why carbon? Carbon (atomic #6) • Abundance on Earth. • Makes 4 covalent bonds because 2 electrons in first shell and 4 in the 2nd shell… needs 4 more e• Versatility of carbon allows for complex shapes & arrangements -Straight chains -Branched chains -Rings • Single, double, or triple bonds too!!!! some carbon compounds glucose Ethyne benzene Glycogen GLUCOSE FUNCTIONAL GROUPS are clusters of atoms attached to the carbon skeleton that influence the properties of the molecules they compose. FUNCTIONAL GROUPS: know this for the SAT subject test • Hydrogen (-H) polar or nonpolar; involved in condensation and hydrolysis. • Amino (-NH2) Basic; involved in peptide bonds • Phosphate (-H2PO4) acid; links nucleotides; energy carrier group ENERGY • Methyl (-CH3) nonpolar, makes molecules hydrophobic CONTROL OF GENE EXPRESSION • Carboxyl (-COOH) acidic (carboxylic acids) BESIDES WATER…WHAT MOLECULE IS INVOLVED IN ALL CHEMICAL REACTIONS??? THIS IS THE ENERGY MOLECULE ASSISTING “coupled to” ALL BIOCHEMICAL REACTIONS??? ATP adenosine tri-phosphate • Energy molecule • Energy is stored in the bonds between the phosphate groups. • Energy is released when the phosphatephosphate bond is broken!!!!! ATP --> energy + ADP + Pi Adenosine TRI phosphate can be broken down into Adenosine DI phosphate… When this happens, energy is released to power ENDERGONIC reactions. NAD+ + H+ + e- + e- = NADH WHAT TYPES OF MOLECULES ARE LIVING THINGS MADE OUT OF ??? (hint: there’s 4 categories) THERE ARE 4 TYPES OF LARGE MOLECULES… each is made from specific kind of smaller, “building block” . 1. PROTEIN 2. CARBOHYDRATES 3. NUCLEIC ACIDS 4. LIPIDS (POLYMERS) 1. 2. 3. 4. amino acids glucoses nucleotides glycerol + fatty acids (MONOMERS) Monomers & Polymers MONOMERS are simple molecules “chemical building blocks” used to construct larger carbon compounds called POLYMERS. Ex. Nucleotides form DNA amino acids form PROTEIN How do we build polymers from monomers? How do we break down polymers into monomers? CONDENSATION REACTION (dehydration synthesis) BUILDS LARGER MOLECULES monomer in, water outyou get a polymer!!! HYDROLYSIS or DISSOCIATION splitting of a polymer by adding a water molecule. - Means “to CUT w/ water”. Macromolecule (polymer) Chemical building block (monomer) carbohydrate s simple sugars (ex. glucose) proteins Amino acids (20 types) lipids Glycerol & fatty acids nucleic acids (DNA, RNA) Nucleotides (A,T,G,C) #1 CARBOHYDRATE functions: 1. Short term energy storage & 2. Provide structure for cell walls cellulose Plants- Starch ex.amylose Animals- glycogen • • • • CARBOHYDRATES Made of C, H, & O. Ratio is 1 Carbon:2 hydrogens:1 Oxygen The number of carbons varies. Can exist as monomers or polymers. 1. Monosaccaride (single sugar) ex. Glucose- metabolized to make ATP Fructose- fruit sugar Galactose- milk sugar 2. Disaccharide (double sugar) ex. Sucrose = fructose + glucose Maltose = glucose + glucose Lactose = galactose + glucose 3. Polysaccharide (3 or more) ex. Glycogen = millions of glucoses in animal cells Starch = millions of glucoses in plant cells Cellulose = millions of glucoses in plant cell walls ISOMERS are molecules with the same molecular formula but different shapes. •C 6 H 12 O6 • Glucose (used for cell energy) • Fructose (fruit sugar) • Galactose (milk sugar) #2 LIPIDS FUNCTIONS: 1)long-term, light weight, energy storage 2) communication & 3) structure #1 triglyceride #2 Steroidhormone #3 phospholipid phospholipid bilayer Cell membrane LIPIDS are: large, nonpolar, organic molecules that don’t dissolve in water. • C,H, 0 but with a higher ratio of carbons & hydrogen than carbohydrates. • Carbon-Hydrogen bonds store energy. • Monomers of LIPIDS are: 1) glycerol & 2) fatty acid(s) Water fearing FATTY ACIDS 2 SIDES: An unbranched carbon chain (12-28 carbons) at one end That is HYDROPHOBIC water fearing Water loving And a -COOH carboxyl group at the other end That is HYDROPHILLIC water loving COMPLEX LIPIDS TRIGLYCERIDE 3 fatty acids + 1 glycerol Saturated ones have a high melting points & solid at room temperatureshortening. Unsaturated ones have lower melting points, are liquid at room temp and are found in plant seeds and fruits. saturated vs. unsaturated Saturated means each carbon is “full of hydrogens”. BAD FOR YOUR HEALTH (beware of hydrogenated products like margarine, shortening and CRISCO) Unsaturated means the carbons have some double bonding and fewer hydrogens. Best = polyunsaturated fats These may lower your PHOSPHOLIPIDS Two fatty acids joined by a molecule of glycerol. A double layer of phospholipids provide a stable and effective barrier for cells. WAXES Long fatty acid joined to a long alcohol chain. Highly waterproof Protective coating in STEROIDS • Four fused carbon rings with various functional groups attached. • Not made of fatty acids. • Many animal hormones are steroids. • Ex. Cholesterol, testosterone, progesterone etc. #3) NUCLEIC ACIDS function is to store and transmit genetic information and use that information to direct the synthesis of new protein DNA RNA NUCLEOTIDES are the basic building block of Nucleic Acids NUCLEOTIDE STRUCTURE: All contain a 1. sugar (ribose or deoxyribose) a 2. phosphate… & a 3. nitrogen containing base . - Adenine - Cytosine - Guanine - Thymine (Uracil in RNA nucleotides) DNA’s sugar is DEOXYRIBOSE RNA’s sugar is RIBOSE DNA… stores genetic information. • THE sugars and phosphates make the “uprights” of the ladder. (joined by covalent bonds) • The nitrogenous bases make the “rungs” of the ladder. (joined by hydrogen bonds) • STORES information used in the manufacture of proteins. • RNA molecules are assembled based on a “gene” (1 side of DNA) Two polymer chains, twisted ladder, spiral staircase, double helix RNA does the work of building proteins!!! • mRNA is the messenger (directions) • tRNA brings the amino acids (transfer RNA) • rRNA builds the RIBOSOME (huge enzyme where the polypeptide chains are built) #4) PROTEINS provide structure & hormonal control and they transport materials like oxygen or materials across cell membranes. PROTEINS POLYPEPTIDE Amino Acid (monomer) • organic compounds made of carbon, hydrogen, and nitrogen. • Ex. Skin, muscles, hair, enzymes, and some hormones. • Made from Amino Acids joined by PEPTIDE bonds. • 20 types • AMINO ACIDS consist of: a central CARBON w/ 4 fun groups 1. hydrogen -H 2. amino group -NH2 3. carboxylic acid -COOH 4. R group (variable x 20) 4 levels of STRUCTURE OF PROTEINS are due to interactions of the amino acids. 1) PRIMARY STRUCTURE Amino acid chain (peptide bonds) 2) SECONDARY STRUCTURE Alpha helix, Beta pleated sheet (due to hydrogen bonds) 3) TERTIARY STRUCTURE 3-D clump (R group interactions) 4) QUATERNARY STRUCTURE Two or more 3D proteins. ENZYMES • • • • Are catalytic PROTEINS Names end in -ase. Biological Catalyst Lower the amount of required activation energy to start RXN. • Thus… speeds up reactions! • Unlike inorganic catalysts which are consumed in the RXN, enzymes are RECYCLED (don’t get used up). Enzymes are catalytic proteins… the protein’s unique primary sequence & the environment of the solution (pH, salts, polarity) results in a unique tertiary globular structure or shape of the protein. • The SUBSTRATE fits into the ACTIVE SITE of the enzyme. • Shape dependent- very specific • LOCK & KEY model means one enzyme works w/ one substrate. • INDUCED FIT model says the enzyme “hugs” the substrate once it binds. • Ex. CATALASE breaks HYDROGEN PEROXIDE into WATER and OXYGEN Temperature Effects rate Of RXN. Think about kinetic energy. • Work best under specific conditions of temperature, pH, and salt concentration. • High temperature or changes in pH, solvent polarity, or Salt concentration can DENATURE the enzyme (now it won’t work) • DENATURE = CHANGE SHAPE Which enzyme works best at acidic pH? • Ubiquitous! • they are everywhere • every reaction has a specific enzyme to catalyse it! 3 DIGESTIVE ENZYMES • Amylase- breaks carbs to sugar • Peptidasebreaks peptide bonds… breaks proteins down into amino acids. • Lipase- breaks lipids apart.