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RECAP • Photosynthesis produces: – Oxygen – Sugar (glucose) – How will these substances be used to make ATP for the organism? Cellular Respiration • Chemical bond energy in food molecules is converted to a form that can be used by the cell (ATP) Energy stored in food is measured in calories – Fats store more energy per gram than do carbohydrates and proteins Cellular Respiration may be: • Anaerobic: doesn’t require oxygen • Aerobic: requires oxygen Anaerobic Respiration • Makes a small amount of usable energy without using oxygen • Reactions occur in cytoplasm • Produces 2 ATP per glucose molecule • Takes place in yeast, and bacteria • Can also occur in human muscle cells if no oxygen is present. Anaerobic Respiration Glycolysis: (sugar is “split”) Glucose is only partially broken down Not all of it’s stored energy is released C6H12O6 + 2 ATP → 2 pyruvic acid + 4 ATP (6-carbon) (3-carbon) Needed to start Net gain of energy is 2 ATP Are produced What Happens to Pyruvic Acid? What happens to Pyruvic Acid? • Fermentation of Alcohol – (Oxygen not present) C6H12O6 → Ethyl Alcohol + CO2 + 2ATP – Happens in Yeast – Pyruvic acid (from split glucose) is turned into ethyl alcohol and CO2 (carbon dioxide) – Useful in the brewing and baking industries • Produces alcohol • Produces carbonation • Bubbles of CO2 make bread rise What happens to Pyruvic Acid? • Fermentation of Lactic Acid: – (Oxygen not present) C6H12O6 → Lactic Acid + 2ATP – Happens in some bacteria • Pyruvic acid (from split glucose) becomes lactic acid • Used to make cheese, yogurt and buttermilk GLYCOLYSIS Alcohol Fermentation Lactic Acid Fermentation Let’s Try This • Activity: Finger “Crunches” – As fast as possible crunch your fingers 100 times Lactic Acid buildup in human muscle cells • During intense exercise, if oxygen is not present, human muscle cells use anaerobic respiration to produce some energy, yielding lactic acid • Lactic acid builds up • Muscles eventually cramp up (burning feeling) Oxygen Debt: • Eventually Lactic acid goes to your liver where it is changed back into glucose which can be used again. • In order to convert the lactic acid, your body needs oxygen When You Die Anaerobic Respiration • Glycolysis happens in cytoplasm • To produce more ATP, (if oxygen present) reactions must take place in the Mitochondria Mitochondria Structure Aerobic Respiration • Makes more ATP using oxygen • Occurs in mitochondria • Most eukaryotic organisms carry out aerobic respiration • Plants also carry out respiration!!! • Glucose gets completely broken down to release energy • Releases more energy than anaerobic respiration (more energy efficient) • Most of the CO2 and H2O you exhale is produced from aerobic respiration C6H12O6 + O2 → CO2 + H2O + 36 ATP Aerobic Respiration Summary 3 Steps of Aerobic Respiration Step 1: Glycolysis – Same as in anaerobic respiration – Doesn’t require oxygen – Takes place in cytoplasm – Glucose is split producing 3-carbon pyruvic acid • Also produces NADH from NAD+ • This “carries” some high energy electrons and H+ – Net production of 2 ATP Step 2: Kreb’s Cycle (Citric Acid Cycle) – Requires oxygen – Pyruvic acid from glycolysis enters mitochondria – Reactions occur in mitochondrial matrix – Produces high energy electron/hydrogen carriers • NADH and FADH2 (called coenzymes) • These molecules carry “H+” and high energy electrons • These will be used later to help produce more ATP – Produces CO2 gas – Produces 2 ATP Aerobic Respiration Summary Step 3: Electron Transport Chain – Requires oxygen – Occurs on inner membrane of mitochondria – Uses high energy electrons and H+ to convert ADP to ATP • Uses the NADH/FADH2 from glycolysis & Krebs cycle – Final electron & hydrogen acceptor is oxygen (which then becomes H2O which is released) – Produces 32 ATP!!! – Produces H2O • Animation of Electron Transport Chain • Note that Oxygen is the final electron acceptor and accepts 2 H+ to become water! • http://vcell.ndsu.nodak.edu/animations/etc/ movie-flash.htm • Respiration Animation • http://www.qcc.cuny.edu/BiologicalScience s/Faculty/DMeyer/respiration.html Anaerobic Phase Doesn’t need O2 Aerobic Phase Needs O2 Efficiency of Cellular Respiration Anaerobic: Yields 2 ATP per glucose – End products of fermentation still contain a lot of unused potential energy – Meets energy needs of simple organisms Aerobic: Yields 36 ATP per glucose – More efficient – 45% of the energy available in glucose is transferred to ATP where it can do further work for the cell – Note: Car engines only converts about 25% of the energy from gasoline Respiration of Fats and Proteins • Get broken down and converted into amino acids, glycerol and fatty acids • These can enter respiration pathway at different points – Fats yield twice as much ATP as glucose – Proteins yield about the same as glucose but are not the preferred energy source for the cell Evolution of Cellular Respiration • First life originated when there was hardly any oxygen in the atmosphere. (Probably prokaryotes with few organelles and no mitochondria) – Glycolysis was the first biochemical process to evolve – Happens in cytoplasm and no oxygen needed. Comparing Photosynthesis and Cellular Respiration • How are these two processes related to each other? • In a typical plant cell describe the way energy is obtained and utilized through both photosynthetic and respiration processes. – Include the materials that need to be taken in and released Comparing Photosynthesis and Respiration