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Feed Inputs to Animals Bob von Bernuth Considerations in a Ration/Diet Energy Proteins & Amino Acids Minerals Vitamins Non-nutritative additives Energy Energy is produced when organic molecules undergo oxidation. C6H12O6 + 6 O2 => 6 CO2 + 6 H2O + energy Proteins and Amino Acids Proteins are amino acids There are limiting amino acids Lysine Methionine A lot of others! They should be balanced Amino acids are the main source of N Limiting amino acids results in reducing N excretion. Animals generally have a dietary requirement for Calcium Chlorine Copper Iodine Iron Magnesium Manganese Phosphorus Potassium Selenium Sodium Sulfur Zinc Importance of Phosphorus • One of the most important minerals required by livestock and poultry. • Complexes with calcium to give rigidity to bones. • An integral part of many organic compounds – plays important roles in energy and protein metabolism. • Almost every biochemical reaction that occurs in muscle, blood and other soft tissues involves phosphorus. • Affects protein synthesis, lean deposition in growing animals. Phosphorus Requirements • • • Best source of unbiased information on requirements is the nutrient requirement publications of the National Research Council (NRC). NRC is part of the National Academy of Sciences – a private organization established 140 years ago by President Abraham Lincoln to advise the nation on issues of science and technology. The Committee on Animal Nutrition of the NRC has established guidelines for feeding animals for past 75 years. Swine versus Dairy Swine are a monogastric Dairy cattle are ruminants Ruminants have a rumen (large stomach) Rumen contains bacteria which produce phytase enzyme—breaks down phytate feedstuffs Nonruminants don’t Swine Considerations Since they don’t have phytase—we have to overfeed P in order to ensure adequate available P. Or—we can add phytase—it works in swine, it just isn’t produced there (it also works for poultry) Or—we can use low phytate corn Phosphorus Essential for skeletal system development Generally low availability Feedstuffs—60-75% is phytate (not available) Corn grain—14% Soybean meal—23-30% What happens to the rest? Nitrogen and Phosphorus in Animal Manures N Manure from: Swine Poultry Beef Dairy Sheep Sweeten (1992). P (% of DM) 4.71 5.13 3.96 3.75 3.89 2.97 1.62 1.07 0.79 0.56 Swine and Poultry Manure High in Phosphorus - Why? • Diets (especially swine diets) generally • • • tend to be oversupplemented with phosphorus. Grains, oilseed meals in swine and poultry diets are high in phytate phosphorus. Pigs and poultry are unable to degrade phytase and utilize the phosphorus - no phytase in their digestive tract. As a result, most of dietary phosphorus from the natural ingredients is excreted in the feces. Phytic Acid – The Culprit O P O O- - O P O O O- - O Phytate Phosphorus O P O O- - O P O O O- - O Phytate Phosphorus Content of Cereal Grains, Byproducts, and Oilseed Meals Phytate Phosphorus % of total P % of total P Barley Oats Corn Wheat Grain sorghum Nelson et al. (1968). 56 56 66 67 68 Wheat bran 70 Wheat middlings 74 Soybean meal 61 Cottonseed meal 70 Sesame meal 81 Nutritional Strategies to Reduce Phosphorus in Swine Manure • Feed diets that are not excessive in • • • • phosphorus. Formulate diets on an “available phosphorus” basis. Use feedstuffs that are low in phytate or that have endogenous phytase. • Wheat, wheat byproducts, triticale, barley. Reduce dietary phosphorus and supplement with phytase. Use low-phytate cereals and oilseed meals. Model-Predicted P Excretion (kg) in Growing-Finishing Pig Fed Corn-Soy Diet from 20 to 120 kg Kilograms of P 1.2 1.0 79% More P Excreted 1.25 0.8 0.6 0.70 0.4 0.2 0.0 NRC (1998) + 0.2% Extra P Phytase – What Does it Do? • Increases phytate digestibility - increases bioavailability of P in cereal grains and oilseed meals. • Reduces the amount of supplemental inorganic P needed to maximize growth and bone mineralization. • Markedly reduces fecal P excretion. • Increases the absorption of Ca, Mg, Zn, and other divalent cations. • May improve the utilization of dietary protein Low-Phytate Corn Forms of Phosphorus in Germ of Normal and Mutant lpa1 Corn 0.5 P in germ, % 0.4 Other Organic P 0.3 Inorganic P 0.2 Phytic Acid P 0.1 0 Normal Raboy et al. (1990) Mutant lpa1 Soluble Carbohydrates in Soybean UDP Sucrose UDP-Glu UDP-Gal Myo-Inositol ADP 1 Sucrose 2 Fructose Glucose + Fructose UDP 3 1 Glu6P ATP 2 Myo-Inositol, 1P Synthase Galactinol Synthase 3 Raffinose Synthase 4 Stachyose Synthase Galactinol Myo-Inositol Phytic Acid Raffinose Galactinol 4 Myo-Inositol Stachyose Dairy Considerations A dairy cow has phytase. Perhaps because of the large amount of feed she eats (50-60 lb/day) some phytase might help. However, we tend to overfeed P. Why Do We Overfeed P? Safety margin Not needed Increased milk yield Doesn’t work Hypophasphatemia Doesn’t help Improves reproduction Data ??? P in feed unknown No excuse You Get Back What You Put In! P has no gaseous phase It can’t escape to the air If you fed it, it’s in the animal, the milk or the excretion. If you manure sample doesn’t show it, you better look for it. Dairy Phosphorus Balance 90 Total Intake Unavailable Potentially Available 9 81 Truly Absorbed Milk 63 29 34 9 + 18 + 34 = 61 Nutrients retained by animals (Elemental) Animal N P K Beef 0.016 0.007 0.002 Dairy 0.012 0.007 0.002 Pork 0.023 0.007 0.002 Layers 0.022 0.006 0.002 Broilers 0.026 0.006 0.002 Turkeys 0.021 0.006 0.002