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Common Defects in Wine Presented by Jef L. Stebben Stebben Wine Consultants Stebben Wine Consultants Formed in 2002 to provide quality winemaking to small producers Consistent focus on quality and improvement at low cost to clients Now working with wineries from 9,000 cases to home winemakers Jef L. Stebben Phone (707) 287-5337 E-mail [email protected] www.stebbenwine.com (Notes from this presentation will be posted on the website.) Overview Reduction in Wine – Sulfides Oxidation in Wine – Volatile Acidity Brettanomyces / Dekkera Sulfides In wine H2S – The most common in wine – – – – – – – – Can form during fermentation Forms in stressed fermentations Low nitrogen musts Utilizes vineyard Sulfur (Residue on skins) Low levels of Vitamins in musts High levels of Cysteine Yeast strain Too much SO2 Mercaptans / Disulfide Mercaptans are stable molecules forming from large amounts of H2S and Ethanol and/or reduction of Amino Acids during storage. Dimethyl Disulfide and Diethyl Disulfide can form from mercaptans and need to be reduced before they will can be removed from wine. Prevention of Sulfides Vineyard Practices – No Sulfur post veraison Harvest sound balanced fruit Add VITAMINS to fermentations Add NUTRIENTS to fermentations Do not add Cu prior to fermentation Rack whites after settling Do not ferment in the presence of Bentonite Removal of Sulfides H2S can be removed by contact with Copper. (CuSO4 or other method) Often the addition of nutrients during fermentation will remove H2S Often racking or splashing the fermentation will remove H2S Removal of Disulfides Perform a test to determine the presence of Disulfides (Cd++, Cu++, and Ascorbic Acid) Use SO2 to reduce Disulfides to Mercaptans Treat with CuSO4 to remove H2S and Mercaptans Samples Hydrogen Sulfide Mercaptan / Disulfide Volatile Acidity Defined as those acids which may be removed from solution by steam distillation Generally taken to mean Acetic Acid May include Carbonic, Sulfurous, Sorbic, Lactic, Formic, Butyric, and Propionic Acids Sources of Volatile Acidity Yeast Lactic Acid Bacteria Acetic Acid Bacteria Conditions that promote VA Wines with residual sugar Wines made from Botrytis infected fruit Wines with Brettanomyces Stuck Fermentations ML fermentation in the presence of sugar High sugar fermentations Conditions that promote VA High temperature fermentations Wines stored with headspace “Cold Soaking” without SO2 “Cold Soaking” without gassing tank And many more… Yeast Kloeckara apiculata / Hanseniaspora uvarum will produce large amounts of Ethyl Acetate Brettanomyces will produce acetic acid Sacharomyces will produce VA in a normal fermentation (about 0.4 g/L) VA production is higher in the presence of Botrytis cineria Bacteria Lactic Acid Bacteria will utilize sugars during fermentation to make VA. They can out-compete yeast and stop a fermentation. Acetic Acid Bacteria can utilize sugars and oxidize ethanol to make VA. They are more common after fermentation. Prevention of VA Cleanliness and good cellar technique Good, clean, and sound fruit Keep up with topping in barrels Overflow barrels when topping, combined with direct addition of SO2 Minimize headspace in tanks Keep up with monthly SO2 additions Removal of VA Mechanical separation of VA from wine – Reverse osmosis, followed by ion exchange Blending Re-fermentation of contaminated wine is not very effective and may spread the contamination Tannin and/or Yeast lees may reduce perception of oxidation in wine Samples Elevated VA in wine Oxidation in wine Brettanomyces/Dekkera One of the most common spoilage yeasts Present in every wine region It has never been isolated in the vineyard Contributes unwanted phenols in wine – 4-ethylguaiacol (4EG) – 4-ethylphenol (4EP) Brettanomyces/Dekkera It can survive on minute amounts of sugars It can metabolize cellobiose (from cooperage) Thrives in wines with RS and low SO2 Prevention of Brett Cleanliness Maintain SO2 and Topping Fermentation Management Control Vectors – Used cooperage – Purchased wine – Pumice and winery waste Brettanomyces Notes SO2 levels over 25 ppm will inhibit enzymes which allow production of 4EP and 4EG SO2 will degrade thiamine required for Brettanomyces growth Removal of Brett Taint Blending of wine is not effective (sensory threshold for taint is 1.6 ppb) Mechanical removal – Reverse Osmosis combined with Ion Exchange Samples 2005 Cabernet Sauvignon 2006 Petite Syrah References Concepts in Wine Technology, Yair Margalit, PhD., The Wine Appreciation Guild, 2004 Production Wine Analysis, Bruce Zoecklein, Kenneth C. Fugelsang, Barry H. Gump, & Fred S. Nury, Van Nostrand Reinhold, 1990 Wine Analysis and Production, Bruce Zoecklein, Kenneth C. Fugelsang, Barry H. Gump, & Fred S. Nury, Aspen Publishers, 1999 Wine Microbiology, Kenneth C. Fugelsang, Chapman and Hall, 1997