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A Biology and Engineering Cooperative Project James W. Stevens M. Karen Newell University of Colorado at Colorado Springs 30 April 2005 general background connection between cellular metabolic pathways and immune system response surface signals tied to mitochondrial activity and fuel cooperative effort biology – science driver – cell cultures growing conditions – cell counting and physical preparation engineering – data acquisition – temperature measurements – data reduction – data smoothing background-this project 2 mouse leukemia lines L1210 – not drug resistant L1210/ddp– drug resistant cultured to 10^6 to 10^8 cells stressed with bleach – measurable, repeatable differences – different culture conditions, metabolic states cumulative heat release L1210ddp L1210 14 12 10 joules 8 6 4 2 0 16:51:36 16:52:19 16:53:02 16:53:46 -2 -4 time 16:54:29 16:55:12 16:55:55 data analysis first law of thermodynamics: dE G Q W dt dT G mc hA(T T ) dt measured heat generation rate L1210ddp L1210 6 5 4 watts 3 2 1 0 16:52:05 16:52:10 16:52:14 16:52:18 -1 -2 time 16:52:23 16:52:27 16:52:31 1 bt* ae y (t*) dt* ce 0.5 0 0 0.2 0.4 0.6 0.8 1 data smoothing least square curve fit: minimize y(t*) yˆ 2 i measured heat generation rate L1210ddp, smoothed L1210, smoothed L1210ddp L1210 6 5 4 watts 3 2 1 0 16:52:05 16:52:10 16:52:14 16:52:18 -1 -2 time 16:52:23 16:52:27 16:52:31 summary leverage complementary capacities– specific to people and project involve students plunge into a new area other cooperative efforts at UCCS biomechanics –biology, mechanical engineering orthopedic mechanics –MERIC, biology, mechanical engineering feedback controls –MAE, biology microgravity – MAE assistive technologies – MATH, biology