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Photosynthesis Main Idea • Light energy is trapped and converted into chemical energy during photosynthesis. Overview • Most autotrophs (only 10% of the Earth’s species) make organic compounds, like sugars, by photosynthesis. • Photosynthesis occurs in two phases. • Overall equation: – 6CO2 + 6H2O C6H12O6 + 6O2 Phase One: Light Reactions • The absorption of light is the first step in photosynthesis. • Two energy storage molecules – NADPH and ATP – are produced to be used in the light independent reaction. Chloroplasts • Large organelles that capture light energy in photosynthetic organisms. • Found mainly in the cells of leaves • Disc-shaped organelles that contain two main compartments essential to photosynthesis Chloroplasts • Thylakoids are flattened saclike membranes that are arranged in stacks. • The stacks are called grana. • Light-dependent reactions occur in the thylakoids. • The stroma is a fluid filled space outside the grana where the light-independent reactions take place. Pigments • Light-absorbing colored molecules called pigments are found in the thylakoid membranes of chloroplasts. • Different pigments absorb specific wavelengths of light. • Violet light has the shortest wavelength, while red light has the longest. • Chlorophylls are the major light absorbing pigments in plants. Pigments • Chlorophyll a and chlorophyll b are the 2 most common types of chlorophyll in autotrophs. • These types of chlorophyll absorb most wavelengths except green. • This means the green light is reflected; therefore, plants look green. Pigments • Other pigments are also found in leaves. • In the fall when the chlorophyll starts to break down, the other pigments are able to be seen. • Hence, the vibrant fall colors on the trees. Electron Transport • The thylakoid membranes have a large surface area, which provides the space needed to hold large numbers of electron-transporting molecules. • Photosystem I and photosystem II contain lightabsorbing pigments and proteins. • Photosystems are composed of photosynthetic pigments clustered together. • Each pigment inside the photosystem is capable of capturing photons, which are packets of energy. Electron Transport • 1. Light energy excites electrons in photosystem II. • 2. Light energy causes a water molecule to split, releasing an electron into the electron transport system, a H+ ion into the thylakoid space, and oxygen as a waste product. THIS SPLITTING IS ESSENTIAL TO PHOTOSYNTHESIS. Electron Transport • 3. The excited electrons move from photosystem II to an electron-acceptor molecule in the thylakoid membrane. • 4. The electron-acceptor molecule tranfers the electron along a series of electroncarriers to photosystem I. Electron Transport • 5. In the presence of light, photosystem I transfers the electron to a protein called ferrodoxin. The electrons lost by photosystem I are replaced by electrons shuffled from photosystem II. • 6. Ferrodoxin transfers the electrons to the electron carrier NADP+, forming the energy storage molecule NADPH. Chemiosmosis • ATP is produced in conjunction with electron transport by the process of chemiosmosis. • The breakdown of water is not only essential for providing the original electron, but for also providing the protons (H+) necessary to drive ATP synthesis during chemiosmosis. Chemiosmosis • Eventually H+ will accumulate in the thylakoid interior and diffuse down the concentration gradient to the stroma through ion channels spanning the membrane. • These channels are enzymes called ATP synthases. • As H+ moves through ATP synthase, ATP is formed in the stroma. What Happens If… • Some of the light-energized electrons may return to chlorophyll after they’ve moved around the electron transport chain, but most continue on with NADPH. • If these electrons are not replaced, the chlorophyll will be unable to absorb light and the light-dependent will stop and no ATP will be made. • To replace lost electrons, water molecules are split by a process call photolysis. Phase Two: The Calvin Cycle • Because NADPH and ATP are not stable enough to store chemical energy, there is a second phase of photosynthesis. • The Calvin cycle is the phase in which energy is stored in organic molecules such as glucose. • AKA the light-independent reactions. Phase Two: The Calvin Cycle • 1. Carbon fixation – 6 CO2 molecules combine with six 5-carbon compounds to form 12 3carbon molecules called 3-phosphoglycerate (3PGA). • 2. Energy in ATP and NADPH is transferred to the 3-PGA molecules to form high energy molecules called glyceraldehyde 3-phosphates (G3P). ATP supplies the phosphate group, while NADPH supplies the H+ ions and electrons. Phase Two: The Calvin Cycle • 3. 2 G3P molecules leave the cycle to be used for the production of glucose and other organic compounds. • 4. An enzyme called rubisco converts the remaining 10 G3P molecules into 5-carbon molecules called ribulose 1, 5biphosphates (RuBP). These molecules combine with new carbon dioxide molecules to continue the cycle. Phase Two: The Calvin Cycle • It takes a total of 6 cycles for one glucose to be formed.