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Photosynthesis Stored Energy What is Photosynthesis? plants convert the energy of sunlight into the energy in the chemical bonds of carbohydrates – sugars and starches. Requirements for Photosynthesis Carbon Dioxide - CO2 Water - H2O Energy – In the form of sunlight 3 Main Stages 1. Energy is captured from sunlight. 2. Light energy is converted to chemical energy (ATP & NADPH) 3. ATP and NADPH power the synthesis of organic molecules, using carbon from carbon dioxide. Where does Photosynthesis occur? Inside plant cells – specifically chloroplasts 1. DNA 2. Ribosomes 3. & 6. Outer Membrane 4. Grana 5. Stroma 7. Starch Grain Chloroplast Thylakoid – flattened, membrane bound sac Grana – stacks of thylakoids Stroma – fluid matrix Light Energy (Energy is captured by sunlight) Electromagnetic Spectrum Radiant Energy – energy that is transmitted in waves that can travel through a vacuum. Electromagnetic spectrum – complete range of radiant energy. PHOTONS! Tiny packets of radiant energy When Photons strike a surface….. 1 – reflected 2 – absorbed 3 – transmitted • Green plants are green because they absorb all of the colors of the visible spectrum except the green color (aka the green wavelengths). Pigments Molecules containing atoms that enable it to absorb light. Types of Pigments Chlorophyll – the primary lightabsorbing agent for photosynthesis Carotenoids – yellow & orange pigments Phycoerythrin – red and blue Photosytems- molecule clusters of pigments found in the thylakoid membranes Photosytem I boost electrons by absorbing light with a wavelength of 700 nm Photosystem II – boosts electrons by absorbing light with a slightly shorter wavelength than 680 nm Stage 2 Light energy is converted to chemical energy… a.k.a. Light – Dependent Reactions Electron Carriers Excited electrons – high energy Special carriers – electron carriers Electron transport chain NADP+ - accepts and holds 2 high energy electrons along with a hydrogen ion (H +) NADP+ + H + = NADPH Light-Dependent Reaction 4 Basic Processes Light absorption Electron transport O2 production ATP formation Light-Dependent Reaction cont. 1. Photons of radiant energy strike PSII Energy is passes to the chlorophyll molecule Excited electron (e-) is boosted to…. 2. A thylakoid membrane protein where… the e- is passed along a series of electron carriers called … the electron transport chain 3. At the end of the ETC, an ATP is released into the stroma 4. PS I gets the e- from PS II. It gets boosted to…. 5. Thylakoid membrane protein where… The e- is passed to the ETC… 6. The ETC passes the e- to the electron carrier NADP+ and is converted to NADPH 7. As e- move from chlorophyll to NADP+, more H+ ions are pumped across the membrane 8. The inside of the thylakoid membrane builds up with + charge 9. Outside the thylakoid is – charged. 10. H+ ions cannot exit without help. They use ATP synthase. 11. Protein channel rotates. 12. As it rotates, ADP binds with a phosphate to make ATP Why doesn’t the chlorophyll run out of e ? Enzymes on the inner side of the thylakoids break up water molecules into 2 electrons, H + ions , and 1 oxygen atom! Light-Dependent Reaction Uses Water ADP NADP+ Produces Oxygen ATP NADPH The Calvin Cycle – sometimes called Light Independent Reactions Plants use the energy that ATP and NADPH contain to build high-energy compounds that can be stored for a long time. Uses ATP and NADPH from the light dependent reactions to produce high-energy sugars. Steps to the Calvin Cycle 6 CO2 molecules enter the cycle from the atmosphere These combine with 6, 5 - carbon molecules to make 12, 3 - carbon molecules The 12 are converted into higher-energy forms (Energy from ATP & NADPH) Calvin Cycle cont. 2 of the 12, 3 – carbon molecules are removed from the cycle to make sugars, lipids, amino acids or other compounds The remaining 10, 3 – carbon molecules are converted back to 6, 5 – carbon molecules.