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Photosynthesis/respiration Living things use energy to maintain homeostasis Photosynthesis = the process by which autotrophs capture energy from the sun and store it in the chemical bonds of glucose. Occurs in chloroplast. Sun energy + 6 CO2 + 12 H2O C6H12O6 + 6 O2 + 6 H2O Respiration = process by which living things break down glucose. The energy from the chemical bonds in glucose are captured in ATP molecules for use by an organism to do work.. Occurs in mitochondria. C6H12O6 + 6 O2 6 CO2 + 6 H2O + ATP energy Redox RX Oxidation/reduction processes = transfer of energy by the loss/gain of electrons OILRIG Oxidation Is Losing Reduction Is Gaining Electrons moved from carrier molecule to carrier molecule Photosynthesis and respiration use redox Rx to convert the suns energy into energy that is usable by living things Photosynthesis Photosynthesis captures energy from the sun and stores the energy in the chemical bonds of glucose. Sun energy + 6 CO2 + 12 H2O C6H12O6 + 6 O2 + 6 H2O Occurs in chloroplasts in mesophyll cells in leaves. CO2 and O2 enter/exit leaves via stomata on leaf surfaces. Combined process of light dependant and light independent Rx Recall structure of chloroplast Inner thylakoid space = Phospholipid bilayer outer membrane pace inside thylakoid Grana = stacks of thylakoids Stroma = space outside thylakoids Chlorophyll = light sensitive pigment. Drives photosynthesis light Electromagnetic radiation (ER) = all the wavelengths () of electromagnetic energy Wavelength () = distance between peak peak of trough trough Higher frequency () = shorter () lower frequency () = longer ( Photon = ‘mass’ of energy contained by all of ER Visible light spectrum ROYGBIV 400-700 nm () Light reflected is what we ‘see’ Other are absorbed Plants use red and blue in photosynthesis Conversion of light energy into the chemical bonds of glucose occurs In the light dependant (photosystem II and photosystem I) and light independent (Calvin cycle) reactions Light dependant RX Generate: NADPH, ATP (by photophosphorylation) and O2 Photosynthetic pigments Photosystem I P700 Chlorophyll a Chlorophyll b carotenoids and Photosystem II P680 Noncyclic light dependant Rx 1. Light hits P680 in photosystem II. 2. Two excited electroms (e-) leave P680 (to a higher energy level) and are captured by a primary e- acceptor. 3. The e- are passed ‘down’ to photosystem I via carrier molecules Plastoquinone (Pq), a cytochrome complex, and plastocyanin. 4. Energy from e- is used to pump H+ from stroma into inner thylakoid space contributing to the production of ATP. 5. When e- reach photosystem I, they join P700 (fill in for missing e-) IN THE MEANTIME An enzyme was used to split water to form H+ ions in inner thylakoid space (2) e- to replace those lost (2) in P680 O2 Noncyclic light dependant Rx Now to continue our story…. 6.. Light hits P700 in photosystem I 7. Two excited electroms (e-) leave P700 (to a higher energy level) and are captured by a primary e- acceptor. 8. Electrons are passed from the primary acceptor to a carrier molecule Ferredoxin (Fd) 9. NADP+ reductase transfers the e- from Fd to NADP to form NADPH What happens to all the H+ building up in the inner thylakoid space? chemiosmosis H+ diffuse down [ ] gradient through ATP synthase To produce ATP (photophosphorylation) Cyclic light dependant Rx Produces ATP but no NADPH 1. Light hits P700 in photosystem I 2. Two excited electroms (e-) leave P700 (to a higher energy level) and are captured by a primary e- acceptor. 3. Electrons are passed ‘down’ and returned to P700 via carrier molecules Fd from photosystem I and cytochrome complex and Pc from photosystem II Energy is used to produce ATP via chemiosmosis ATP and NADPH are used in the carbon fixation pathway known as the light independent Rx or Calvin cycle Light independent Rx Calvin Cycle ATP and NADPH are used to produce glyceraldehyde 3-phosphate from CO2 (3) Carbon fixation rubisco 3 CO2 + 3 RuBP (ribulose biphosphate) 6 3-phosphoglycerate Reduction (6) 3-phosphoglycerate + ATP (6) 1,3-biphosphoglycerate (6) 1,3-biphosphoglycerate + NADPH (6) G3P (1) G3P become glucose or other compounds (5) G3P to regenerate RuBP Regeneration (5) G3P + ATP 3 RuBP Calvin cycle ready to begin again Evolution in photosynthesis C3 plants Carbon fixation involves rubisco Hot dry weather CO2 as stomata close to reduce water loss via transpiration O2 can combine with rubisco and be sent to Calvin cycle instead of CO2 Causing photorespiration = BAD Photorespiration = product broken down with no ADP formation Loss of G3P production No RuBP generated C4 plants C4 plants Bundle sheath cells arranged around veins of leaves Mesophyll cells between bundle sheaths and leaf surface PEP carboxylase In mesophyl : CO2 + PEP (phosphoenolpyruvate) oxaloacetate (4C) Efficient carbon fixation Fixed carbon brought to bundle sheaths and released to Calvin cycle Minimizes photorespiration and increases photosynthetic productivity CAM plants CAM plants = crassulacean acid plants succulents As in C4 plants, CO2 is fixed into intermediate organic molecules Carbon fixation takes place at night when stomata are open Carbon released into Calvin cycle during the day (stomata closed) when light is available for ATP, NADPH production