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Chapter 10 Autotrophs vs. Heterotrophs Photosynthesis: conversion of light energy into chemical energy (sugar) ◦ carbon dioxide + water + light ---> sugar + oxygen ◦ Energy converting process...not primary ATP producer for autotrophs ◦ Two reactions... Light reactions and Calvin cycle Chloroplasts. ◦ concentrated in leaves ◦ found in mesophyll ◦ within chloroplast are thylakoids, which increase surface area for reactions this is where the light reactions of photosynthesis take place ◦ Fluid surrounding thylakoids in chloroplast is Stroma. this is where Calvin cycle takes place Sunlight. ◦ electromagnetic energy that travels in waves. Visible spectrum most important to life. ◦ light organized into photons (particle with fixed quantity of energy) short wavelength = more energy long wavelength = less energy Photosynthetic pigments. ◦ substances that absorb visible light ◦ color we see is wavelength of light that is reflected and not absorbed. plants appear green because the pigments absorb red and violet-blue light and reflect green ◦ each pigment absorbs a slightly different wavelength, increasing the spectrum of colors to drive photosynthesis chlorophyll a chlorophyll b carotenoids Occurs in the thylakoid membrane and thylakoid space. Reactions are organized into two photosystems (protein complexes that absorb energy from sunlight) ◦ photosystem II and photosystem I In general... ◦ Use water and sunlight to produce oxygen and ATP ◦ transfer electrons from light energy to electron carriers. NADP+ (nicotinamide adenine dinucleotide phosphate) converted to NADPH How light reactions work... ◦ absorption of light excites electrons in chlorophylls (pigment) ◦ Photosystems transfer these electrons through ETC creates H+ gradient, ATP synthesized ◦ electrons are also used to create NADPH, carrying electrons to Calvin Cycle http://highered.mheducation.com/olc/dl/1200 72/bio13.swf Anabolic process. Builds carbohydrates and consumes energy. Occurs in the stroma of the chloroplast ◦ Takes carbon dioxide and produces a carbohydrate reaction catalyzed by enzyme rubisco only works with NADPH and ATP produced from the light reactions With photosynthesis, some water is lost via the stomata (where gas exchange takes place) ◦ On hot days, stomata close to prevent excess water loss…but it also limits gas exchange and photosynthetic activity ◦ C3 plants (normal photosynthesis) Produce less sugar on hot, dry days (rice, wheat) ◦ C4 plants (alternate photosynthesis) Unique leaf anatomy that allows for alternate pathway-able to synthesize CO2 Still able to produce sugar on hot, dry days (corn, sugarcane) ◦ CAM Plants (succulents) Open stomata at night…close during day Two steps of photosynthesis occur at different times CO2 is stored in other parts of plant (as organic compounds) Organisms that have the ability to synthesize organic compounds without sunlight or oxygen ◦ Take CO2 or CH4 with H2S and produce carbon compounds for energy ◦ Mostly these organisms are found on the ocean floor (bacteria) Carbon dioxide + water + hydrogen sulfide sugar + sulfuric acid (sulfur compound)