Download black sea ecosystem-an important target and source of

BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND
SOURCE OF BIOCHEMICAL COMPOUNDS AND
BIOPRODUCTS
PhD biochem. Daniela Mariana Rosioru
Head of Marine Biochemistry Laboratory
National Institute for Marine Research and Development “Grigore Antipa”,
300 Mamaia Blvd., RO-900581, Constanţa, Romania.
E-mail: [email protected]
31.05 - 2.06.2016
STANDArD-Murighiol, Romania
Marine biotechnology concerns the environment and marine organisms
evaluation, starting with their cultivation until their processing in order to
obtain useful bioproducts in various branches.
Based on the rich tradition and background in marine biochemistry, the
National Institute for Marine Research and Development (NIMRD)“Grigore
Antipa” Constanta, Romania, has developed in time many research
directions for the exploitation of marine living resources, which included the
main marine organisms from the Romanian Black Sea area.
The utilization of marine resources in the medical-pharmaceutical domain as
a result of originality of the methods for proceeding the raw material and the
good quality of obtained bioproducts already requested on the market,
represents one of the NIMRD concerns in this field.
31.05 - 2.06.2016
STANDArD-Murighiol, Romania
First applied biochemistry research in marine field
was started by GAVRILESCU in 1950:
-determination of vitamin D in oil extract from shark
liver, in order to know the antirachitic properties
-capitalization of fish fat for pharmaceutical purposes
Between 1969 and 1970 TELEMBICI et al. researched
the content in ascorbic acid (vitamin C), glucose and
glycogen in mollusks.
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Since 1970, the concerns of biochemistry have led to
investigations
on
the
identification,
isolation
and
characterization of active biological products from marine
organisms in the Black Sea.
•alginic acid and mannitol content in Cystoseira barbata,
proteolytic activity in mollusks (MARZA, 1972, 1976),
•elaboration of a process for obtaining sodium alginate from
seaweed (BOGHICI and STATE 1972),
•1971-1981- analysis of lipid fractions from mussels, Mya
arenaria and decapod crustaceans (MOLNAR, 1974; ROSOIU,
1975; MARZA et SERBAN, 1981)
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•determining of sulphomucopolysaccharide in mollusks (MOLNAR
et al.1974,1976)
•determining glycogen and amino acids in Mytilus galloprovincialis
(MARZA, 1972; MARZA et al.1976, 1981,1989)
•determining of amino acids and protein content in unicellular algae
(VOINESCU, 1976)
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STANDArD-Murighiol, Romania
Purification and biochemical characterization in terms of enzymatic
extracts from marine invertebrates and fish:
-amylase (ROSOIU, 1976-1978)
-glucosidase (MARZA si SERBAN, 1981)
-cellulase-hemicellulase (MARZA et al. 1977, 1981, MARZA 1979-1980)
-carbohydrases (MARZA si SERBAN, 1978)
- developing a process for extraction of amylase from mussel
hepatopancreas (IORDACHESCU et. al 1975, 1977)
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STANDArD-Murighiol, Romania
-study of the physical, chemical and pharmacological properties of
some products excreted by organisms in seawater (MARZA, 1988)
-obtaining of the natural inhibitors of pepsin, trypsin and chymotrypsin
from Rapana venosa (ROSOIU, 1981-1990)
-isolation, extraction and purification of hyaluronidase from the viscera
and gonads of marine fish followed by developing a method for
obtaining
the substances with an anti-hyaluronidase action,
anti-rheumatic and anti-inflammatory properties known as a
pharmaceutical product ALFLUTOP (ROSOIU, 1981-1990)
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STANDArD-Murighiol, Romania
- study of active principles in celenterate species (Mnemiopsis leidyi
and Aurelia aurita), in the crustacean Artemia salina from Techirghiol
Lake, in fish species Trygon pastinaca (CRASMARU et al., 1994, 1997)
and influence on the human body
TOMA et al. (1996), ZANOAGA et al. (1996) studied the content of
albumin, globulin and glutelin in mollusks flesh.
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Within the BIOTECH project “Biotechnological methods for utilisation of
some marine resources used in medical and industrial fields“ NIMRD
analysed the BLACK SEA offer - sea water and marine organisms to
process them as REMEDIES, ADJUVANTS, NUTRITIVE SUPPLEMENTS.
( S.C. LABORATOARELE MEDICA S.R.L. )
REMEDIES - represent resources and methods able to act under the most
favourable circumstances on living organisms in order to heal or prevent
an illness.
NUTRITIVE SUPPLEMENTS and COMPLEMENTARS - are the concentrated
sources of nutritive or other substances with nutritive and physiological
effects, alone or in combination
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The SEA WATER presence in natural sanogenic practices it is not a
novelty, as it is well known its healing, anti-inflammatory and depurative
effects in heliomarine cures.
Nasal washable solutions – instillation:
• NASOMER - marine solution, aseptic filtrate, with rectified pH;
• NASOMER PROP - marine solution, aseptic filtrate, with rectified pH and
propolis;
• NASOMER FORTE - marine solution, aseptic filtrate, with rectified pH
and Echinacea extract;
• THINNING AGENTS and DEPURATIVE for nasal mucous membrane for
allergic rhinitis, acute and chronic rhino-sinusitis.
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All products are recommended for mouth cavity affections, aphta (ulcer in
the mouth), pharynges - amygdalitis, gingivitis - stomatitis, acute on
chronic.
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Gargles
•APIMER - marine solution with propolis tincture;
•APIMER FORTE - marine solution with propolis and Echinacea tincture.
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MARINE ALGAE-organic deposits, containing marine macroalgae on
beaches, after storms.
ALGE MARINE- for medical
treatment of lung, heart, thyroid
gland,
circulatory
diseases,
physical anaemia and physic
asthenia.
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MARINE EPIBIOSIS- represents a life community formed by organisms
which are fixed on submerged alive or not alive structures (mollusks ,
shellfish, algae )
BIOMER, EPIBIOZA MARINA- for recovery, extended intellectual and
physical effort, prevention and control of the aging phenomena.
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MUSSELS (Mytilus galloprovincialis)
Ions of calcium, magnesium
and iron have were extracted
from marine mollusk shells
and fixed as chloride, lactate,
levulinate and, together with
other bioactive components,
embedded
in
a
food
supplement called RĂDUCU,
useful in the treatment of
anemia, rickets and tetanus
related to lack of calcium.
RHEUMAMER-protects
the
conjunctive tissues, having, in
the
same
time,
antiinflammatory properties.
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STANDArD-Murighiol, Romania
MARINE FISH
Marine resources like goby (Gobius miger) or hanus (Neobius
cephalanga - nostomus) are common species along Romanian
seaside of the Black Sea, with recognised nutritive value.
ARTHROMER-adjuvant in arthrosis, spondylosis, rheumatic polyartrits
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STANDArD-Murighiol, Romania
SHARK CARTILAGE
CARTILAJ DE RECHIN-arthritis,
diabetic retinopathy, adjuvant in
chemotherapy,
osteoporosis,
immunosuppression, psoriasis
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BIOTECH project
“Biotechnology for new derma-cosmetic products based on peptideprotein complex from marine resources which are unused for food chain”
• obtaining of derma- cosmetic products from roe and viscera of fish from
the Black Sea and unusable raw materials in the food industry.
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The intermediate products obtained in the research project are of two
categories:
- DERMOMAR 1 (peptide - protein complex)
- DERMOMAR 2 (lipid-protein complex),
• further serve to diversify the range of ointments by introducing
components and additives at user choice and availability.
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NIMRD - ”NUCLEU” PROGRAM - ONGOING PROJECT
The research on the biotechnological
exploitation of some marine resources from
Romanian Black Sea Coast
Project director: PhD biochem. Daniela Mariana Rosioru
31.05 - 2.06.2016
STANDArD-Murighiol, Romania
“Ovidius” University Constanta
• Recovery of seaweed biomass in the Black
Sea to obtain new nano - bio- pharmaceutical
formula of innovative therapies for diseases
of the soft tissues of the oral- facial system
31.05 - 2.06.2016
STANDArD-Murighiol, Romania
Patents for pharmaceuticals
•
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•
•
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PRODUS HEPATOPROTECTOR, PRODUS HIPOCOLESTEROLEMIANT OSIM
80321
AMILAZE OSIM 68328/75140
CELULAZE HEMICELULARE OSIM 65398-70071
MEDICAMENT ANTIULCEROS OSIM 82876/90968/96784
COMPLEX ENZIMATIC PROTEOLITIC OSIM 98420
HIALURONIDAZA SI MEDICAMENT TROMBOLITIC OSIM 86312/90969/102703
COMPLEX CU ACTIVITATE ANTIHIALURONIDAZICA SI MEDICAMENTE
CONDITIONATE GAMA ALFLUTOP-INJECTABIL, UZ EXTERN, APA DE GURA
OSIM 82273/92641/98316/106239/108165
PRODUS ANTISCLEROTIC OSIM 8023
ULEIURI FARMACEUTICE OSIM 88044/166333
Monografia SUBSTANTE BIOLOGIC ACTIVE DIN ORGANISME MARINE,1992
N.Rosoiu, M.Serban
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STANDArD-Murighiol, Romania
FURTHER?
 the permanent evaluation of the availability and free access to raw
natural material from Black Sea
 the sustainable utilization of the marine offer
 the developing of the informational and organizational
infrastructure in order to improve an accurate connection man –
ecosphere
31.05 - 2.06.2016
STANDArD-Murighiol, Romania
31.05 - 2.06.2016
STANDArD-Murighiol, Romania