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Download Double-Chamber Plethysmographs Respiratory Systems Respiration
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respiratory systems Double-Chamber Plethysmographs PY2 73-2416 Differential Pressure Transducer DP45-14 or DLP2.5 CFBA/TAM-A HSE PLUGSYS • Specific airway resistance measurement together with the standard parameters tidal volume and respiratory rate, in conscious animals • Can also be used as head-out plethysmograph with aerosol challenge possibility • Aerosol challenge • Applications: – Toxicology – Environmental studies – Long term drug effects This plethysmograph box has been specially developed for the investigation of bronchospas-molytically active substances on the conscious animal. The awake animal is placed into the double chamber and is restricted so that the head protrudes into the front chamber. The neck is sealed with a soft diaphragm. The nasal air flow is measured in the front chamber, the thoracic respiratory flow in the rear chamber. Both measurements are made with measurement screens and differential pressure transducers. There is a phase shift between the two respiratory flows from which the specific airway resistance after PENNOCK is calculated by HSE respiratory software PULMODYN ‘PENNOCK’. The standard parameters like respiratory rate, tidal volume, peak inspiratory and expiratory flow, inspiratory and expiratory duration are evaluated from the flow measured in the thoracic chamber. The nasal chamber is connected to the bias flow venturi-jet tube (requires stable compressed air, we recommend use of the PLUGSYS module PRM PY2 73-0195 in a housing PY2 73-1520 ), and can be equipped with an aerosol nebulizer and oxygen flushing (supply required). To reduce the handling of valves and switches the PCU Module PY2 73-1748 and PY2 73-1749 can be used. The thoracic chamber can be removed and the animal can easily be placed into position. The chamber is available in two different sizes to suit different size animals. If only the thoracic chamber is used for measurement, the plethysmograph box is a head-out box with the possibility of aerosol application. Head-Out Application of Double-Chamber Plethysmograph • Measurement of standard respiratory parameters tidal volume and respiratory rate, in conscious animals • Breathing patterns evaluated: Calculation of Time of brake, Time of Pause, Airflow at 0.5VTE • Aerosol challenge • Applications: – Toxicology – Environmental studies – Long term drug effects respiratory systems Double-Chamber Plethysmograph for Restrained Rats/Guinea Pigs Respiration HSE PULMODYN Software This is a basic non-invasive method for measurement of standard respiratory parameters tidal volume and respiratory rate, in conscious animals. The guinea-pig (or rat) is placed in a Double-Chamber plethysmograph box. The thoracic respiratory flow is measured in the rear chamber with measurement screens and differential pressure transducer. A neck cuff seals the thoracic chamber. The nasal chamber can be left open by removing the front panel of the nasal box so that the animal breathes free air. The nasal chamber can also be connected to the bias flow venturi-jet tube (requires stable compressed air, we recommend use of the PLUGSYS module PRM PY2 73-0195 in a housing PY2 73-1520 ), and can be equipped with an aerosol nebulizer and oxygen flushing (supply required) for aerosol applications. To reduce the handling of valves and switches the PCU Module PY2 73-1748 and PY2 73-1749 can be used. The following parameters can be calculated from the measured pressure fluctuations in the thoracic chamber: • Peak Insp. Flow, Peak Exp. Flow, Tidal Volume, Minute Volume, Respiration Rate Optionally: • Duration of Inspiration, Duration of Expiration, Apnea Time, Time of brake, Time of pause, Airflow at 0.5TV Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com F88
