Download TI: Biosystematic Study of Genus Nicotiana in Egypt Based on

TI: Biosystematic Study of Genus Nicotiana in Egypt Based on Fingerprints of
RAPD and Isozyme.
AU: Ahmed Mohamed Fawzy and Wafaa Mahrous Amer
La: English
Da: October (2005)
PU: Egyptian Journal of Biotechnology 21:187-202.
ABSTRACT: The loss of biodiversity has become an issue of great global concern,
especially the wild relatives of the cultivated economic species. Genus
Nicotiana(Solanaceae) is among the genera of high potentiality in the
Egyptian flora. The genus represented in Egypt by sections; each of them
represented by one species: three wild Nicotiana species namly: N.glauca
Graham, N. plumbaginifolia Viviani and N. rustica L., while N.tabacum L.
(tobacco) is the only cultivated species. Sixteen RAPD markers and two
isozymes were used to assess the genetic variation and inter-specific
relationships among tobacco and its wild relatives in Egypt. The
polymorphism in RAPD and isozyme bands were used to construct the
phylogenetic dendrogram. The inter-specific relationship reflects that: (1) The
genetic improvement of the cultivated N.tabacum can be achieved using its
closely wild relative (N. plumbaginifolia). (2) Conservation of wild croprelatives is an urgent topic to secure the gene pool of the economic species.
Key wards: Nicotiana, RAPD,Isozyme, Solanaceae