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Proceedings of the IV th International Congress of Ethnobotany (ICEB 2005), 2006, 397-406 Differences between written and archaeological record: The case of plant micro remains recovered at a Northwestern Argentinean Pipe CAPPARELLI, Aylen1, M. Lelia POCHETTINO2, Andreoni DIEGO1, and Rubén D. ITURRIZA3 1 Scientific Department of Archaeology, Natural Sciences Museum of La Plata, Paseo del Bosque s/n, (1900), La Plata, Argentina [email protected] 2 Laboratorio de Etnobotánica y Botánica Aplicada, 64 y 120, (1900) La Plata; Argentina [email protected] 3 Municipalidad de Londres de Quimivil, (4753) Londres, Argentina There are two main objectives of this paper. The first one is to identify fragmented and carbonised material stuck to the inner part of the tube of a pipe from the site La Puntilla in Catamarca Province. This pipe is characteristic of the Lower Formative Period (650 BC – 500 AD) of Northwestern Argentina. Two requirements are essential in the identification of this kind of material: 1-knowledge of those plants traditionally recorded (in documentary evidence) to have been used in such artifacts, and 2- an appropriate methodology for the identification of those residues that lack external morphology sufficient to allow them to be macroscopically referred to a specific plant. By means of a wide range of chemical treatments, several plant structures were distinguished from this pipe such as: papillae with thick walls, interlaced vegetal and animal fibers, crystals, trichomes of different morphology and function, different kind of epidermis and starch grains. These structures were thought to belong to fragmented and carbonised leaves and ground seeds of E. coca, leaves of Nicotiana sp., leaves of possibly Aloysia citriodora and Ilex sp., as yet unidentified glandular trichomes. Almost no evidence of smoking plants are mentioned in written evidence from the Argentinean NW, contrasting with the archaeological record. Just a few species are mentioned from the neighboring Chaco region, mainly belonging to the genus Nicotiana. Therefore, our second objective is to discuss the differences that appear between archaeological and written record. Key words: archaeological pipes, biodynamic plants, archaeobotany, written record, Northwestern Argentina Approximately 200 pre-Hispanic pipes were recovered from NW Argentina, specifically from the altiplano of Jujuy, Salta and Catamarca, the ravines of El Toro and Humahuaca, and the sub-Andean border of the basin of the San Francisco river; as well as from valleys, such as the Calchaquí, Yocavil, Hualfín, Ambato, Abaucán, Famatina, Andalgalá, Sanagasta and Vinchina (Fig. 1). Most of them belong to the Lower Formative (650 BC500 AD) according to Nuñez Regueiro (1975). All pipes recovered from NW Argentina are spread among official and private collections (Fernández, Ravina–, and Balesta 1999). The oldest pipes belong to the Pre-Ceramic -or Paleo- Indian- caves from the East of the Humahuaca ravine –Inca Cueva IC c7 and Huachichocana CHIII sites–. From the first one, two tubular bone-carved pipes were recovered, and dating from 2130 years BC (Fig. 2A). From the second site, four tubular stone carved pipes were estimated to belong to 2000 years BC (Fernández Distel 1980). It is thought that the pipe smoking costume of the aborigines of NW Argentina during the Pre-ceramic/PaleoIndian period may have come from the Chaco region (Pérez Gollán and Gordillo 1993) or from the Bolivian lowlands- western Paraguay (Dougherty 1972) and survived for several centuries. By the Lower Formative (650 BC-500 AD) the several pipes recovered are represented in different cultural bodies such as Tebenquiche, La Ciénaga, Saujil, Tafí, Condorhuasi, Las Cuevas, and Laguna Blanca, among others (Fernández, Ravina–, and Balesta 1999). Apparently, the large extension of the basins of the Pilcomayo, Bermejo, and Pasaje-Juramento rivers assisted in this diffusion (Pérez Gollán and Gordillo 1993). The 398 Proceedings of ICEB 2005 oldest pipe of this period was found in the San Francisco culture, and was dated at 620 years BC (Dougherty 1972). Finally, just a few pipes are represented in the archaeological record of the Upper Formative (500-900 AD), specifically in La Aguada culture (Fig. 2E). Interestingly, pipes were no longer to be found in NW Argentina after the Formative Period, being replaced in the archaeological record apparently by the “rape” complex costume that might have come from the Tiwanaku cultures (Pérez Gollán and Gordillo 1993). Outside the NW, some pipes were recovered in Argentina from the Cuyo valleys of Jachal (Punta de Barro) and Uspallata, as well as from the Mesopotamia littoral (Fernández, Ravina–, and Balesta 1999:11) (Fig. 1). Pipes from Argentina were made from different raw materials: bone, stone and ceramic. The oldest ones from Inca Cueva were made from large -apparently human- bones (two pipes) and from stone (four pipes). Meanwhile, from 620 years BC onwards ceramic pipes were the most abundant, although stone pipes were also present. Their shapes vary, ranging from tubular ones, which present a shape of simple tubes (Fig. 2A), ethnographically common in the Mesopotamia and Chaco-; to those called “monitor”, bearing a boatshape base in whose center raises the bowl –ethnographically common between Mapuches, Huiliches and Tehuelches, and sites of the SE Bolivia, although it was also recovered from NW Argentina- (Fig. 2C); to the angular forms (Fig. 2B,D,E), with a horizontal and vertical branches that ends in a burner or bowl –more common in the East of Brazil, N Argentina, Patagonia and Chile-. Some ceramic pieces feature an articulate vertical branch (Fig. 2D). The decoration applied to these objects may include different techniques, such as incisions, painted, shaped or a combination of several of them. Decoration is usually applied to the vertical branch, to the bowl and seldom to the horizontal branch. In the pipes bearing sculpture decoration, anthropomorphic and zoomorphic motives prevail. The faces have very prominent noses with well-defined nostrils and they may also present prominent jaws. Eyes differ between almond-shape eyes to rounded holes. They all show open mouths with very prominent teeth (Fernández, Ravina–, and Balesta 1999:23). Fig. 1 Map of Argentina showing the location of La Puntilla site, in the Catamarca Province. Major divisions correspond to the different archaeological areas of the country (sensu González 1977). White words show the main valleys and ravines where pipes were recovered. Since the beginning of the last century, the function of these pipes has been subject to intense debate. Some considered them as receptacles where certain substances were burnt for ritual purposes, therefore labeling them as “incensarios” (censers) (Debenedetti in Bregante 1926). Others argue that they were used to smoke such vegetal substances, thus calling them “pipas” (pipes) (Debenedetti 1931; Márquez Miranda 1946). This second hypothesis seems to be confirmed today, because different kinds of archaeological information indicate thus. Such is the case of the Rupestrian art’s representation of human figures smoking a pipe (petroglyphs) of Laguna Blanca (Catamarca), as well as smoking animal motives –probably monkeys- found in pottery from La Ciénaga cemeteries Capparelli et al.: Differences between written and archaeological record 399 (Catamarca). The latter displays three figures with zoomorphic bodies and anthropomorphic heads smoking pipes with the bowl at an angle (Fernández, Ravina–, and Balesta 1999:33) (Fig. 3). The hypothesis mentioned before, about the Chaco/jungle provenience of the pipe-smoking custom would endorse the presence in La Ciénaga cemeteries of other traits proceeding from those places, such as children burials in urns (Fernández, Ravina–, and Balesta 1999:33). With respect to the chronicles and ethnographic information, there are several sets of data from the Mesopotamia, Chaco, and Patagonian Argentinean regions, that registered how pipes were and are use by aborigines, and which plants are involved in this process. However, only scarce information is registered from NW Argentina. This fact may be related to the absence of pipes in the archaeological record since 900 AD onwards, what might imply both, the possible absence of their use during this period and the omission of their mention by chroniclers. The taxa mentioned by ethnographers as used for smoking in pipes in Argentina include: Anadenanthera, Nicotiana, Dortenia brasiliensis, Berberis and Ilex (Table 1). Nevertheless there is no Fig. 2 Different kind of pipes recovered in Argentina. A. Pre-ceramic tubular bone pipes from the Humahuaca ravine (Inca Cueva site). B. Lower Formative (La Ciénaga culture) stone angular pipe from the Pipanaco basin (La Puntilla site). C. Lower Formative monitor stone pipe from the Pipanaco basin (Tuscamayo). D. Angular (ang.) and angular articulated pipes (art.). E. Upper Formative (La Aguada culture) ceramic angular pipe from the Ambato valley (Rodeo Grande site). Fig. A was taken from Fernández Distel (1980), while C,D, E were taken from Fernández, Ravina–, and Balesta (1999). Fig. 3 Incised smoking animals –possibly monkeys- on La Ciénaga cemetery pottery. Taken from Fernández, Ravina–, and Balesta (1999). Wichís Wichís Tehuelches Argentina Patagonia Paraguay Chaco Boreal Maká Argentina Patagonia Argentina Patagonia Nicotiana tabacum Nicotiana sp. Ilex sp. Nicotiana sp. Berberis sp. Nicotiana sp. Mapuches y Huiliches Mapuches y Huiliches Tehuelches Ranqueles Argentina Patagonia finak Tabaco yerba mate Tabaco calafate Tabaco Nicotiana sylvestris Tabaco, yu’kwas chaguar Tabaco, ‘qoyik coro, koro Tabaco Tabaco Tabaco yerba paraguaya coro, koro Argentina NW Argentina Patagonia Family Solanaceae Solanaceae Aquifoliaceae Solanaceae Berberidaceae Solanaceae Solanaceae Moraceae Solanaceae Solanaceae Bromeliaceae Solanaceae Solanaceae Solanaceae Solanaceae Aquifoliaceae jataj (vilca, cebil) Fabaceae Common name Dortenia brasiliensis Higuerilla Nicotiana langsdorffi Nicotiana tabacum Several species Nicotiana sp. Nicotiana sp. Nicotiana sp. Ilex paraguariensis Nicotiana? Trichocline? Nicotiana? Trichocline? Nicotiana tabacum Scientific name Anadenanthera sp. Argentina Mesopotamia Argentina Mesopotamia Argentina Chaco Argentina Chaco Argentina Chaco Chorotes Matacos Argentina Chaco Chaco Chaco Chaco Chaco Chaco Tobas, Matacos Tobas, Matacos Tobas Argentina Argentina Argentina Argentina Argentina Archaeological Ethnicity area Argentina Chaco Matacos Country leaves leaves stem leaves bark leaves leaves leaves fibrovascular bundle root leaves leaves root leaves leaves leaves leaves root seeds Part used A substitute of tobacco is mixed with sawdust and Ilex paraguariensis stems mixed with Polygonum, Tessaria, Celtis or Sapium when the tobacco is strong or scarce mixed with tobacco mixed with tobacco mixed with Calafate bark used as a filter for cleaning the smoke grinded sometimes mixed with other species mixed with tobacco dried, grinded and mixed with tobacco mixed with flavouring bark mixed with jataj (cebil) mixed with “coro” Processing Arenas (1982:223-4) Gancedo (1973) Mansilla in: Pérez Gollán and Gordillo (1993) Musters in Gancedo (1973) Cooper (1949) Serrano (1934) Suggested by the Botanist Parodi in: Serrano (1934) Suggested by the Botanist Parodi in: Serrano (1934) Cooper (1949) Arenas (2003:343-4) Arenas (2003:344) Arenas (2003:343) Califano (1976:47) Pérez Gollán and Gordillo (1993) Dobrizhoffer (1967) Alanis in: Pérez Gollán and Gordillo (1993) Serrano (1934) Califano (1976:47) Sources Table 1. Plants used in pipes in Argentina and neighboring countries according to ethnographic record (except Parodi in Serrano 1934) 400 Proceedings of ICEB 2005 Chaco Boreal Chaco Boreal Chaco Boreal Chaco Boreal Chaco Chaco Chaco Chaco Chaco Chaco Paraguay Paraguay Paraguay Paraguay Paraguay Paraguay Paraguay Paraguay Paraguay Paraguay Nicotiana rustica? Nicotiana tabacum ? Nicotiana undulata ? South America South America Aristotelia maqui South America Araucanos Chile Eugenia pitra Nicotiana paniculata? Araucanos Chile South America Araucanos Chile Anadenanthera Araucanos Chile Nicotiana sp. Deinacanthon urbanianum Sapium haematospermum Celtis pallida Polygonum hispidum, P. punctatum Ruprechtia triflora Nicotiana tabacum Sapium haematospermum Celtis pallida, C. spinosa Tessaria dodoneaefolia Polygonum paraguayense Species Scientific name Chile Araucanos Chile Lengua-Maskoy Lengua-Maskoy Lengua-Maskoy Lengua-Maskoy Lengua-Maskoy Lengua-Maskoy Maká Maká Maká Maká Archaeological Ethnia Area Country cevil maqui Pitra molle Papa silvestre tabaco Tàmom’a tooting amhe Winak yaamìt waata heena setuk qanaxataqakuk cofok Cahaq tituwey Common name Solanaceae Solanaceae Solanaceae Solanaceae Fabaceeae Eleocarpaceae Mirtaceae Anacardiaceae Solanaceae Bromeliaceae Euphorbiaceae Celtidaceae Polygonaceae Polygonaceae Solanaceae Euphorbiaceae Celtidaceae Asteraceae Poligonaceae Family leaves leaves leaves leaves seeds leaves leaves lleaves fibrovascular bundle leaves leaves leaves leaves leaves leaves leaves leaves leaves Part used Arenas (1981:82-3) Arenas (1981:82-3) Arenas (1981:82-3) Arenas (1981:82-3) Arenas (1981:82-3) Arenas (1981:82-3) Arenas (1982:223-4) Arenas (1982:223-4) Arenas (1982:223-4) Arenas (1982:223-4) Sources mixed with tobacco mixed with tobacco mixed with tobacco mixed with tobacco Cooper (1949) Cooper (1949) Cooper (1949) Cooper (1949) Dobrizhoffer (1967) Serrano (1934) Serrano (1934) Serrano (1934) Serrano (1934) mixed with papa silvestre, Serrano (1934) maqui, pitra or molle used as a filter for cleaning the smoke mixed with tobacco mixed with tobacco mixed with tobacco mixed with tobacco mixed with Polygonum, Celtis, Sapium when tobacco is scarce mixed with tobacco mixed with tobacco mixed with tobacco mixed with tobacco Processing Table 1. (cont.) Plants used in pipes in Argentina and neighbouring countries according to ethnographic record (except Parodi in Serrano 1934) Capparelli et al.: Differences between written and archaeological record 401 Proceedings of ICEB 2005 402 Table 2. Chemical evidence provided by NW Argentinean archaeological pipes Artifact Akcaloids Species Family Sources Bone pipes IC c7 N,N dimethiltryptamine (=Bufotenine) Anadenanthera sp. Fabaceae Fernández Distel 1980:65 None evidence of N,N dimethiltryptamine was found Stone pipes CH III The alkaloids that might have been represented by the main peak of the gass chromatography were: N-Methylpavine Norcoralydine Tetra hydropalmanine Xylopine O-Methyl-caseadina Argemonine Fumaritine Glaucine Hunnemannine micro, nor macro, remains analysis of any Argentinean NW pipe as there have been performed with other artifacts (Pochettino and Capparelli 2004), and only the deposits of the tube of just two pipes (the oldest ones) were selected to look at alkaloids by gas chromatography. One of them demonstrated the presence of N,N dimethiltryptamine, which is also known as Bufotenine, characteristical of Anadenanthera (Table 2). In order to contribute to the interpretation of these elements, there are two main objectives of this paper. The first one is to identify fragmented and carbonized material stuck to the inner part of the tube of a pipe from the site La Puntilla. Two requirements are essential for the identification of this kind of material: 1-knowledge of those plants traditionally recorded (in documentary evidence) to have been used in such artifacts, and 2- an appropriate methodology for the identification of those residues that lack external morphology sufficient to allow them to be macroscopically referred to a specific plant organ or taxon. The second objective of this paper is to discuss the differences between the archaeological versus ethnographical record. La Puntilla site belongs to the Lower Formative Period (650 BC-500 AD) of Northwestern Argentina. A rescue excavation was carried out by one of the authors of this paper during the year 1996, previously to the construction of a large pool to retain water for irrigation. A mortuary context of a man in genuflexion was registered together indeterminated Mendonça 1980:79 with domestic features. Findings also included La Ciénaga II (or Güisyschi) and ordinary pottery, a bronze plaque, “suri” egg and Dassypodidae fragments, and a pipe bowl with anthropomorphic shape, among others. The last remain mentioned was thought at the beginning to be articulated to the pipe analyzed here (Pochettino and Capparelli 2004), but this assumption was after discarded because the association of ceramic with stone material in the same pipe is not usual. Materials and methods The inner part of the horizontal branch of the pipe was scratched by means of a metallic instrument that was previously sterilized with sodium hypochlorite. Methodology used for identification of plant remains depends on the sample state and recovery conditions. Problems arise when the material is highly fragmented or powdered (for instance, material recovered from pipes and inner parts of receptacles, and often further obscured by desiccation or charring, with fragment size less than 500 μm. Preservation of diagnostic tissue organization under these conditions is unlikely. Investigations have shown that diagnostic results can however be obtained, if such samples are first treated with a range of reagents or solvents. Strong oxidizing agents, such as 100v hydrogen peroxide, sodium hypochlorite, lactic acid, potassium permanganate-hydrochloric acid mixtures Capparelli et al.: Differences between written and archaeological record 403 Fig. 4 Coca (Erythroxylum coca). A,B. Starch from seeds. A. Archaeological, B. Reference material. C,D. Papillae in leaves. C. Archaeological. D. Reference. E,F. Crystals in middle rib. E. Archaeological. F. Reference. and reducing agents such as phenol and chloral hydrate, were tested. In most cases samples were washed with sodium hydroxide after treatment. Partial discoloration was thus attained, enabling observation of some histological features, and consequently the identification of the sample by light microscopy. For scanning electron microscope observations the material was firstly washed with alcohol and then with hydrochloric acid to eliminate carbonates, which are common in samples that were embedded in substratum or ashes. Results By means of carrying out this wide range of chemical treatments, several plant structures were distinguished from this pipe such as: – Single or 2-3 compound starch grains, isolated or in amilaceous parenchyma (Fig. 4 A, B), rounded papillae with thick walls (Fig. 4 C, D), presence of cubic crystals by the veins (Fig 4 E, F). They were thought to belong to fragmented and carbonised leaves and ground seeds of E. coca. – Stomata and hairs (Fig. 5 A-B), pluricellular branched trichomes (Fig. 5 C-F) similar to those depicted, for Nicotiana epidermis and named type B1 and B2, by Goodspeed (1954) (Fig. 5 G). – Adpressed hairs similar, but not exclusive, of Aloysia citriodora (Fig. 6 A), trichomes of leguminous morphology and multicelular hairs yet not identified (Fig. 6 D). – Two different kind of epidermis: with festooned cells similar, but not exclusive, of Aloysia citriodora (Fig. 6 B) and with striate cuticle that is similar (but not exclusive) to the one of Ilex paraguariensis (Fig. 6 C). – interlaced vegetal and animal fibres (commonly dyed) (Fig. 6 E). Discussion and conclusion The variety of species recovered from the tube of the pipe may involved two possibilities. On the one hand, the different species might have been smoked/burnt simultaneously under the form of a mixture. On the other hand, the identified plant diversity might have been the result of successive uses of a single plant, which was a different plant in any case. The first possibility implies the use of a mixture made from some substances close to coca and tobacco or a close related species of Nicotiana, together with other different plant parts 404 Proceedings of ICEB 2005 Fig. 5 Nicotiana sp. A,B. Stomata and hairs from leaves. A. Archaeological, B. Reference material. C,D,E. Hairs type B1, B2 and B3 from leaves and petioles. C,D. Archaeological, E. Reference material sensu Goodspeed (1954). for smoking. In this way, just a few species are mentioned in written evidence from Argentina to have been used in pipes, from which tobacco (Nicotiana sp.), cebil (Anadenanthera) and koro (Trichocline? Nicotiana?) are the most frequently reported for the North (Pérez Gollán and Gordillo 1993). As is evident from this work, coca is not mentioned for smoking uses in Argentina. Just two references, both reported by Jussieu (2001, website), were found about the mixing of coca and tobacco. One comes from the Amazon Nonoyu aborigines who mixed tobacco and coca leaves for chewing. The other comes from the Witotos Boras aborigines and several other tribes of northwest Amazonas where ambil –a thick syrup made from tobacco- is “…applied to the gums with a finger or a stick and is swallowed very slowly with saliva and coca powder…”. However, an unconscious mixed of these two species may have been due, if the person who is going to smoke tobacco is/was chewing coca, and carried out a pre-treatment of chewing to the Nicotiana leaves before putting them into the pipe. This pretreatment was reported, for example, by Arenas (1982: 223-224) between the Maká aborigines of Paraguay. Capparelli et al.: Differences between written and archaeological record 405 Fig. 6 Other archaeological remains founded in the pipe. A. Adpressed hair (cedrón/Aloysia citriodora?). B. Festooned epidermis (Aloysia citriodora?). C. Multicelular hairs indet. D. Epidermis with stomata and striate cuticle (yerba mate/ Ilex sp.?). E. Vegetal fibers and wool. The presence of Nicotiana does not imply the use of N. tabacum. Other species were reported to have been used in South America (see for example Cooper 1949). In the Catamarca province grow nowadays several wild species of this genus, as for example: N. cavanilliesi, N. longibracteata, N. corymbosa, N. pauciflora, N. acuminata, N. miersii, N. glauca, N. sylvestris, N. longiflora, N. noctiflora and N. petunioides (Goodspeed 1954). The presence of interlaced plant and animal fibres may be the consequence of two situations. On the one hand the plant dried mix used for smoking in this pipe could have been previously placed within a woven container. For example, the Whichís commonly used bags for putting on the tobacco leaves while smoking (Arenas 2003:344). On the other hand, a filter could have been made from fibers for retaining the solid fraction when smoking. This situation was usual also between the Whichís in Argentina (Arenas 2003:344) or the Lengua-Maskoy in Paraguay (Arenas 1981: 141-42), which preferred the Bromeliaceas fibers. A third possibility explaining the presence of these fibers could have been done by the use of an instrument for cleaning the inner tube of the pipe after their use. Some of the other species identified are seldom mentioned by written record. For example Gancedo (1973) reported the used of Ilex paraguariensis (yerba mate, yerba paraguaya), mixed with tobacco, between the Tehuelches of the Patagonia (Table 1). Dobrizhoffer (1967:206-9) also mentioned that a Spaniard captain that share many journeys with him, smoke “yerba paraguaya” when he lacked tobacco. He said that no pipe was need for that, instead, the man make a cigarette rolling a leave within other rectangular one, by the same manner they used to do with tobacco. In Mississippi cultures of U.S.A. the use of other species of Ilex, I. cassine, was registered by Swanton (1911). The cedrón, also known as hierba luisa (Aloysia citriodora) is not mentioned in the ethnographic data. However, flavoring plants seldom were used for tobacco, as for example the Berberis and other barks (Table 1). Besides unidentified structures are not coincident with any of the ethnographically mentioned taxa. Therefore it seems that only written record is not enough, and that further archaeobotanical studies are still necessary, to reach the correct interpretation of this kind of remains. 406 Proceedings of ICEB 2005 Literature Cited Arenas, P. 1981. Etnobotánica Lengua-Maskoy. Fundación para la Educación, La Ciencia y La Cultura, Buenos Aires. ———. 1982. 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