Download System Superior Reproducibility for Complex Gradients

Waters Alliance System
961169
Superior Reproducibility for
Complex Gradients
Amino Acid
Complex gradients are often required for
difficult separations. The unique design and
control software of the Waters Alliance
Separations Module provides capabilities to
accurately and reproducibly generate the most
complex gradient profiles, such as the AccQTagTM amino acid separation demonstrated
below. Seventeen hydrolysate amino acids
are separated with a five step gradient profile
and the results show excellent reproducibility.
Note that this performance is a clear
improvement over any traditional HPLC
systems. Standard deviations are reported in
seconds.
Std Dev
(seconds)
AMQ
Asp
Ser
Glu
Gly
His
NH3
Arg
Thr
Ala
Pro
Cys
Tyr
Val
Met
Lys
Ile
Leu
Phe
Waters Alliance System consisting of:
Waters Alliance Separations Module
Waters 474 Scanning Fluorescence
Detector with 5uL Flow Cell
1.3
1.8
1.5
1.8
1.7
2.0
1.8
0.8
0.7
0.7
0.9
1.1
1.0
1.0
1.0
0.8
0.8
0.9
1.3
Refer to AccQ-Tag brochure for eluent
descriptions.
N=10
Phe
Val
Arg
Ile
Thr
200
Leu
Met
Ala
Tyr
mV
Lys
100
Glu
Ser
Asp
His
Gly
Pro
NH2
Cys
AMQ
0
10
20
Minutes
30
Copyright 2003 Waters Corporation
Fast Gradient Chromatography
Retention Time Reproducibility
800
Symmetry C18, 3.9 x 50 mm
Flow Rate: 1.5 mL/min
N=17
Std Dev Rt <0.3 sec
Leu
Ile
Phe
(Br)2Tyr
(Int Std)
600
mV
200
Orn Lys
Met
Tyr Val
400
Cys
0
6.00
7.00
8.00
9.00
10.00
Fast gradients often challenge an instrument's ability to deliver the
rapid eluent changes necessary for a reproducible separation. The
low volume, fast cycling time of the Waters Alliance Separations
Module's gradient proportioning valve, coupled with advanced control
software and efficient mixing provides capability for generating fast
gradients. Several rapid gradient separations have been developed
for the analysis of selected amino acids. In the top example the flow is
increased to 1.5 mL/min, and the reproducibility remains excellent.
The bottom example is a fast gradient developed to perform a three
minute gradient at 4 mL/min. Both of these examples illustrate the
superior gradient performance of the Waters Alliance Separations
Module for all gradient profiles.
120
Symmetry C18, 3.9 x 50 mm
Flow Rate: 4 mL/min
N=10
Std Dev Rt <0.6 sec
60
40
80
mV
40
Tyr
Ile
Val Met
Orn
Leu
Phe
Lys
Int. Std.
0
%B
20
0
1.00
2.00
3.00
Minutes
Waters, Alliance and Symmetry are registered trademarks of Waters Corporation. AccQ.Tag is a trademark of Waters
Corporation. Copyright 2003 Waters Corporation Printed in the U.S.A.
WPP204
720000285EN