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GTN INTERBIO EXCHANGE/TRAINING APPLICATION FORM
1. IDENTIFICATION OF INTERBIO PARTNERS AND CONTACT POINTS
Sender:
Interbio contact
person
Spain
Maite López
[email protected]
Recipient:
Interbio contact
person
Portugal
Alexandra Veiga
[email protected]
OTHER REGIONS INVOLVED: Identify
2. CONTACT DETAILS OF LABORATORIES/SERVICES/PLATFORMS INVOLVED
SENDER
RECIPIENT
Lab head
Carmen Muñoz-Almagro
Raquel Sá-Leão
Address
Molecular Microbiology
Department
Pº sant Joan de Deu nº 2
08950 Esplugues, Barcelona
Spain
[email protected]
Laboratory of Molecular
Microbiology – ITQB
Rua da Quinta Grande, 6, Oeiras
2780-156 Oeiras
Portugal
[email protected]
Contact details
3. DETAILS OF THE EXCHANGE
Name of visitior Carmen Muñoz-Almagro
Position Head of Pediatric Infectious Diseases Group-Sant Joan de Déu
Foundation
From 15-07-2011
to
30-07-2011
Workplan
Please provide a short description (~300 words) of the purpose of the visit and an outline of
the work plan
Collaboration between laboratory of molecular microbiology of human pathogens unit,
Instituto de tecnología química e biologíca (ITQB), Universidades nova de Lisboa (UNL),
Oeiras, Portugal and Pediatric infectious diseases research group (PIDRG-HSJD), Hospital
Sant Joan de Déu, University of Barcelona.
Dr. Raquel Sá-Leão from the Laboratory of Molecular Microbiology of ITQB and Prof.
Hermínia de Lencastre head of the Molecular Microbiology of Human Pathogens Unit are
worldwide recognized authorities in the field of pneumococcal infections. They have
important experience about genetic studies related to the diversity of pneumococcal
populations.
PIDRG-HSJD is an emergent research group in the field of pneumococci in Spain. This group
has a well characterized collection of invasive and carriage pneumococcal isolates among
children from Catalonia, during the period 2004-2009. In addition, the molecular
microbiology department of HSJD has wide experience in the use of molecular microbiology
techniques such as Real-Time PCR and sequence-based methods for the diagnosis and
characterization of main pediatric infections. In the field of pneumococci, Real-Time PCR is
applied for diagnosis of pneumococci in direct biological samples (plasma, cerebrospinal
fluid or pleural fluid among others) molecular capsular type detection of pneumococci and
pneumococcal bacterial load quantification. Automatic sequencing is applied for clonal type
detection of pneumococci by MLST.
The objective of this collaboration is to exchange information and know-how through handon experiments about:
1) Real-Time PCR applications for pneumococcal detection and molecular serotyping
2) Serotyping methods based on bacterial culture
3) Detection and characterization of atypical pneumococci
4) Application of Simpson’s diversity index and other coefficients to compare pneumococcal
collections
To develop this collaboration, Dra. Carmen Muñoz-Almagro, Head of PIDRG-HSJD and
responsible of the Molecular Microbiology Department of HSJD, will make a formative
internship next July 2011 in ITQB in Oeiras, Portugal.
This stage will serve to complete the study “ Invasiveness of pneumococcal serotypes and
clones circulating in Catalonia during heptavalent conjugate vaccine period” performed by
Carmen Muñoz-Almagro and the PIDRG-HSJD under the supervision and collaboration of Dr
Raquel Sá-Leão and Prof. Hermínia de Lencastre.
Budget estimation
Please provide an estimation of the expenses you may incur. Remember that you will have to
justify all expenses with the corresponding invoices.
The estimate cost ( room hotel accommodation, meals and transport costs) is 3000€
Date: 22-02-2011
Annex: 1 page cv of applicant
CURRICULUM VITAE
FAMILY NAME, FORENAMES, TITLES: Carmen Muñoz-Almagro, MD, PhD
DATA OF BIRTH: 18-07-1964
POSITION:
-
Head of Molecular Microbiology Department, University Hospital Sant Joan de Déu since
1997
Head of Pediatric Infectious Diseases Group, Sant Joan de Deu Foundation since 2006
WORK ADRESS: Molecular Microbiology Department
University Hospital Sant Joan de Deu
Pº Sant Joan de Deu nº 2
08950 Esplugues, Barcelona
Work phone: +34932805569/ Work FAX: +34932803626
MAIL: [email protected]
ACADEMIC HISTORY
Medical Doctor by Navarra University 1988
PhD by University of Barcelona, 2005
PREVIOUS OCCUPATIONAL EXPERIENCE
1988-1990. Pediatric Medical Practitioner.INSALUD. Ciudad-Real
1991-1993. Clinical training in Microbiology and Infectious Disease Specialist (MIR) University
Hospital Sant Joan de Déu.
1994-1997. Microbiologist at Microbiology Department. Hospital Sant Joan de Deu.
SELECTED 10 PUBLICATIONS
Muñoz-Almagro C, Selva L, Pallares R.Influence of pneumococcal vaccine on the incidence of
empyema. Curr Opin Pulm Med. 2010 Jul;16(4):394-8
This paper is a review about the influence of pneumococcal vaccine on the increase of empyema
observed in the vaccine period
Selva L, Esteva C, Gené A, de Sevilla MF, Hernandez-Bou S, Muñoz-Almagro Direct detection of
Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction. C.Diagn
Microbiol Infect Dis. 2010 Feb;66(2):204-6
This is an article about the usefulness of Real Time for rapid detection of S.pneumoniae. This
technique will be use for direct detection of pneumococci from dried spot in the present study
Muñoz-Almagro C, Esteva C, de Sevilla MF, Selva L, Gene A, Pallares R Emergence of invasive
pneumococcal disease caused by multidrug-resistant serotype 19A among children in Barcelona..J
Infect. 2009 Aug;59(2):75-82
The study compares the incidence of serotype 19A before and after the introduction of conjugate
pneumocccal vaccine. The conclusion of the study reported that in our geographical area there was
an increase of IPD caused by S. pneumoniae serotype 19A which was mainly related with the
emergence of preexisting clones several of them closely related with international multidrug-resistant
clones.
Hernández-Bou S, García-García JJ, Esteva C, Gené A, Luaces C, Muñoz-Almagro C. Pediatric
parapneumonic pleural effusion: epidemiology, clinical characteristics, and microbiological diagnosis.
Pediatr Pulmonol. 2009 Dec;44(12):1192-20
The objective of the study was to describe the epidemiology and clinical characteristics of
parapneumonic pleural efussion by using Real-Time PCR during 2003-2006. One of the conclusions
was that The use of Real-Time PCR to detect S. pneumoniae substantially increases etiologic
diagnosis.
Obando I, Muñoz-Almagro C, Arroyo LA, Tarrago D, Sanchez-Tatay D, Moreno-Perez, D, Dhillon SS,
Esteva C, Hernandez-Bou S, Garcia-Garcia JJ, Hausdorff WP, Brueggemann AB. Pediatric
parapneumonic empyema, Spain. Emerg Infect Dis. 2008; 14(9):1390-7.
This is a collaborative study which report the emergence of empyema in Spain is mainly due to an
epidemic clone of serotype 1. Serotype 1 is not included in the 7-valent conjugate vaccine (PCV7)
Muñoz-Almagro C, Jordan I, Gene A, Latorre C, Garcia-Garcia JJ, Pallares R. Emergence of invasive
pneumococcal disease caused by nonvaccine serotypes in the era of 7-valent conjugate vaccine. Clin
Infect Dis.2008; 46(2):174-82.
This is a major article with more than 80 citations. It is one of the first studies worldwide about
replacement phenomenon. We reported that since the introduction of PCV7 for children, there has
been an emergence of IPD caused by virulent clones of non-PCV7 serotypes that has been associated
with significant clinical changes and a decrease in antibiotic resistance in our geographical area.
Tarragó D, Fenoll A, Sánchez-Tatay D, Arroyo LA, Muñoz-Almagro C, Esteva C, Hausdorff WP, Casal J,
Obando I. Identification of pneumococcal serotypes from culture-negative clinical specimens by
novel real-time PCR. Clin Microbiol Infect. 2008 Sep; 14(9):828-34.
The development and clinical evaluation of real-time Multiplex PCR assay to detect and serotyping
S.pneumoniae is reported in this paper. It was possible to differentiate serotypes 1, 3, 5, 4, 6A, 6B,
7F/A, 8, 9V/A/N/L, 14, 15B/C, 18C/B, 19A, 19F/B/C, 23F and 23A from direct samples. This technique
will be used for direct serotyping of dried spot samples in the present study
Soriano F, Perez-Trallero E, Pallares R, Meseguer MA, Fleites A, Gene A, Gonzalez A, Linares J,
Esteban J, Baquero F, Valdes E, Munoz-Almagro C; Spanish Pneumococcal Infection Study Network.
Streptococcus pneumoniae endophthalmitis: a study of 36 cases with special reference to antibiotic
resistance and treatment options. Clinical Microbiological and Infection. 2006 Jun12(6):519-26.
This is a collaborative study about pneumococcal endophthalmitis performed by the Spanish
pneumococcal network. The conclusion of the study was “ pneumococcal endophthalmitis is a medical
emergency because of the often aggressive clinical course, poor visual outcome and need for
evisceration in a large proportion of patients”
del Campo R, Morosini MI, de la Pedrosa EG, Fenoll A, Munoz-Almagro C, Maiz L, Baquero F, Canton
R; Spanish Pneumococcal Infection Study Network Population structure, antimicrobial resistance, and
mutation frequencies of Streptococcus pneumoniae isolates from cystic fibrosis patients.. Journal
Clinical Microbiology. 2005 May;43(5):2207-14.
This is a collaborative study about pneumococcal infection in cystic fibrosis patients performed by the
Spanish pneumococcal network. We report a long-term clonal persistence and variability, high
frequency of antibiotic resistance, and hypermutability of S. pneumoniae in lung of cystic fibrosis
patients
Latorre C, Gene A, Juncosa T, Munoz-Almagro C, Gonzalez-Cuevas A. Characterisation of invasive
pneumococcal isolates in Catalan children up to 5 years of age, 1989-2000. Clinical Microbiological
and Infection. 2004 Feb;10(2):177-81.
Resistance to penicillin, cefotaxime tetracycline, chloramphenicol and erythromycin as well as
serogroup/type, were related to age and pathology of a cohort of pediatric patients attended to our
Hospital. Strains from children aged < 2 years showed the highest penicillin resistance rate.
PARTICIPATION IN SELECTED RESEARCH PROJECTS
PROJECT TITLE: Influencia de la deficiencia de MBL (Mannose-Binding Lectin) en la enfermedad
neumocócica invasiva y asociación con los diferentes serotipos en población adulta e
infantil.FINANCIAL ENTITY: Fondo Investigaciones Sanitarias (FIS)
LENGHT: January 2011 from
hasta: 31 diciembre 2013 PRINCIPAL INVESTIGATOR: Carmen MuñozAlmagro
PROJECT TITLE: Support laboratory for molecular surveillance of Bordetella pertussi in
Catalonia.FINANCIAL ENTITY: Departament de salut. Generalitat de Catalunya
LENGHT: Since January 2011--- PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Support laboratory for molecular surveillance of meningococcal disease in
Catalonia.FINANCIAL ENTITY: Departament de salut. Generalitat de Catalunya
LENGHT: Since January 2011--- PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Grupo Emergente de Investigación en Enfermedades Infecciosas Pediátricas
FINANCIAL ENTITY: AGAUR, Generalitat de Catalunya, LENGHT .January 2009 from 31 December
2013. PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Support laboratory for molecular surveillance of invasive pneumococcal disease in
Catalonia.FINANCIAL ENTITY: Departament de salut. Generalitat de Catalunya
LENGHT: Since December 2008--- PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Epidemiología Molecular de la infección neumocócica en población
pediátrica.FINANCIAL ENTITY: Caja de Navarra.LENGHT: January 2007 from 31 December 2010.
PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Evaluación de la efectividad de la vacuna antineumocócica conjugada 7-valente en la
prevención de la enfermedad neumocócica invasora en niños menores de 5 años. Estudio de casos y
controles.FINANCIAL ENTITY: Fondo Investigaciones Sanitarias (FIS)
LENGHT: January 2007 from
hasta: 31 diciembre 2009 PRINCIPAL INVESTIGATOR: Ángela
Domínguez
PROJECT TITLE: Detección de la enfermedad invasiva severa pediátrica por Streptococcus
pneumoniae mediante Real-Time PCR. Criterios de gravedad y relación con la carga bacteriana en
los pacientes ingresados en la unidad de cuidados intensivos pediátricos.
FINANCIAL ENTITY: Academia de Ciencias Médicas de Cataluña y Baleares
LENGHT: January 2006 from december 2006. PRINCIPAL INVESTIGATOR: Carmen Muñoz-Almagro
PROJECT TITLE: Estudio de la infeccion y colonizacion por streptococcus pneumoniae: aspectos
epidemiologicos, clinicos, microbiologicos, moleculares e inmunologicos. (Red Española de estudio
de la infección Neumocócica). FINANCIAL ENTITY: Fondo de Investigaciones sanitarias. LENGHT:
January 2004 from december 2006. PRINCIPAL INVESTIGATOR: Román Pallarés
STAYS IN INTERNATIONALLY RECOGNIZED CENTRES
CENTRE: Michigan State University
PLACE: Molecular Microbiology Department
COUNTRY: USA
YEAR: 2002
LENGTH: 1 month
TOPIC: Molecular Techniques
KEY: Student
OTHER PUBLICATIONS IN THE LAST 3 YEARS
A (Article); R (Review); B (Book)
Launes C, García-García JJ, Jordán I, Martínez-Planas A, Selva L,
Muñoz-Almagro C. 2009 INFLUENZA A H1N1 INFECTIONS: DELAYS IN STARTING TREATMENT WITH
OSELTAMIVIR WERE ASSOCIATED WITH A MORE SEVERE DISEASE. Pediatr Infect Dis J. 2010 Dec 30.
[Epub ahead of print].
Fortuny C, Noguera-Julian A, Alsina L, Bellido R, Sánchez E, Muñoz-Almagro C,Ruiz L, Jiménez R.
IMPACT OF CD4 T CELL COUNT ON THE OUTCOME OF PLANNED TREATMENT INTERRUPTIONS IN
EARLY-TREATED HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CHILDREN. Pediatr Infect Dis J. 2010
Oct 27.
Muñoz-Almagro C, Gala S, Selva L, Jordan I, Tarragó D, Pallares R. DNA
bacterial load in children and adolescents with pneumococcal pneumonia and empyema. Eur J Clin
Microbiol Infect Dis. 2011 Mar;30(3):327-35. Epub 2010 Oct
Gonzalez-Bosquet E, Selva L, Sabria J, Pallares L, Almeida L, Muñoz-Almagro C,Lailla JM. Predictive
factors for the detection of CIN II-III in the follow-up of women with CIN I. Eur J Gynaecol Oncol.
2010;31(4):369-71.
Muñoz-Almagro C, Selva L, Sanchez CJ, Esteva C, de Sevilla MF, Pallares R, Orihuela CJ. PsrP, a
protective pneumococcal antigen, is highly prevalent in children with pneumonia and is strongly
associated with clonal type. Clin Vaccine Immunol. 2010 Nov;17(11):1672-8.
Noguera A, Morén C, Rovira N, Sànchez E, Garrabou G, Nicolàs M, Muñoz-Almagro C, Cardellach F,
Miró O, Fortuny C. Evolution of mitochondrial DNA content after planned interruption of HAART in
HIV-infected pediatric patients. AIDS Res Hum Retroviruses. 2010 Sep;26(9):1015-8.
Launes C, Rives S, Catala A, Berrueco R, Toll T, Camos M, Muñoz-Almagro C, Garcia-Garcia JJ, Estella J.
Pandemic influenza A (2009 H1N1) in children with acute lymphoblastic leukaemia. Br J Haematol.
2010 Mar 21 (A)
Sánchez Torrent L, Triviño Rodriguez M, Suero Toledano P, Claret Teruel G, Muñoz Almargo C,
Martínez Sánchez L, Jordán García I, García García JJNovel Influenza A (H1N1) encephalitis in a 3
month-old infant.Infection. 2010 Jun;38(3):227-9. (A)
Muñoz-Almagro C, Martin A. Despistaje de infecciones oportunistas e indicaciones de profilaxis
primaria. En Recomendaciones para el seguimiento del paciente pediátrico infectado por el virus de
la inmunodeficiencia humana (VIH). Ministerio de Sanidad y POLITICA Social Junio 2009. Gobierno de
España. (CB)
Hernández-Bou S, García-García JJ, Esteva C, Gené A, Luaces C, Almagro CM Pediatric
parapneumonic pleural effusion: epidemiology, clinical characteristics, and microbiological diagnosis.
Pediatr Pulmonol. 2009 Dec;44(12):1192-200 (A)
Fernández de Sevilla M, Alayeto J, Fernández Y, Muñoz-Almagro C, Luaces C, García-García JJ [Low
prevalence of Mycoplasma pneumoniae infection in children with acute pharyngitis].Enferm Infecc
Microbiol Clin. 2009 Aug-Sep;27(7):403-5. (A)
Jordán I, Esteva C, Esteban E, Noguera A, García JJ, Muñoz-Almagro C.Severe enterovirus disease in
febrile neonates. Enferm Infecc Microbiol Clin. 2009 Aug-Sep;27(7):399-402. (A)
Selva L, Gonzalez-Bosquet E, Rodriguez-Plata MT, Esteva C, Suñol M, Muñoz-Almagro C . Detection of
human papillomavirus infection in women attending a colposcopy clinic.. Diagn Microbiol Infect Dis.
2009 Aug;64(4):416-21 (A)
Munoz-Almagro C, Rodriguez-Plata MT, Marin S, Esteva C, Esteban E, Gene A, Gelabert G, Jordan
I.Polymerase chain reaction for diagnosis and serogrouping of meningococcal disease in children.
Diagn Microbiol Infect Dis. 2009 Feb; 63(2):148-54. (A)
Muñoz-Almagro C, Muñoz-Fernandez MA. Parâmetros de laboratório para guiar El tratamiento
antiretroviral. Em recomendaciones SPNS/CEVIHP/SEIP/AEP respecto AL tratamiento antiretroviral
em niños y adolescentes infectados por El VIH. Ministerio de Sanidad y Consumo. Marzo 2008.
Gobierno de España. (CB)
Muñoz-Almagro C, Jordan I, Cambra FJ, Esteban E, Urrea M, Garcia-Garcia JJ, Palomeque A.
Quantitative real-time PCR in paediatric patients with herpes simplex infections of the central
nervous system. J Virol Methods. 2008;147(2):297-300. (A)
Kee C, Palladino S, Kay I, Pryce TM, Murray R, Rello J, Gallego M, Lujan M, Muñoz-Almagro C, Waterer
GW. Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus
pneumoniae in patients with community-acquired pneumonia. Diagn Microbiol Infect Dis. 2008;
61(1):72-5. (A)
Alsina L, Gala-Peralta S, Noguera A, Pons M, Séculi JL, Muñoz-Almagro C.Neonatal pulmonary
tuberculosis evolving to a destroyed lung.Int J Tuberc Lung Dis. 2008; 12(5):573-5. (A)
Claret G, Noguera A, González-Cuevas A, García-García JJ, Fortuny C, Muñoz-Almagro C. The
prevalence of GB virus C/hepatitis G virus RNA among healthy and HCV-infected Catalan children. Eur
J Pediatr. 2008 Sep; 167(9):991-4. (A)
Pino-Ramírez RM, Pertierra-Cortada A, Iriondo-Sanz M, Krauel-Vidal X, Muñoz-Almagro C.Neonatal
echovirus 30 infection associated with severe hepatitis in twin neonates. Pediatr Infect Dis J. 2008
Jan;27(1):88. (A)
Camps M, Ricart S, Dimova V, Rovira N, Muñoz-Almagro C, Garcia JJ, Pons-Odena,M, Marcos MA,
Pumarola T. Prevalence of human metapneumovirus among hospitalized children younger than
1year in Catalonia, Spain.J Med Virol. 2008 Aug;80(8):1452-60. (A)
Gonzalez-Bosquet E, Esteva C, Muñoz-Almagro C, Ferrer P, Pérez M, Lailla JM. Identification of
vaccine human papillomavirus genotypes in squamous intraepithelial lesions (CIN2-3).Gynecol
Oncol. 2008 Oct; 111(1):9-12. (A)
Gonzalez-Bosquet E, Esteva C, Muñoz-Almagro C, Lailla JM. Comparison of conization and limited
excision of the transformation zone (LETZ) in the treatment of squamous intraepithelial lesions (SIL)
of the uterine cervix. Eur J Gynaecol Oncol. 2008; 29(2):123-5. (A)
Gonzalez-Bosquet E, Torres A, Busquets M, Esteva C, Muñoz-Almagro C, Lailla JM. Prognostic factors
for the development of vaginal intraepithelial neoplasia.Eur J Gynaecol Oncol. 2008; 29(1):43-5. (A)
Muñoz-Almagro C, Esteva C, Rodriguez-Plata MT, Jordan I, Garcia-Garcia JJ.Pneumococcal Invasive
Disease in Children: Effect of Vaccination Clin Pulm Med: 2008;15(4):197-200 (R)
HONOURS AND AWARDS
Award to the best publication year 2009. Hospital Sant Joan de Déu “Emergence of invasive
pneumococcal disease caused by multidrug-resistant serotype 19A among children in Barcelona.
Journal of Infection 2009
Award to the best publication year 2008. Hospital Sant Joan de Déu “Emergence of invasive
pneumococcal disease caused by nonvaccine serotypes in the era of 7-valent conjugate vaccine”
Clinical Infectious Diseases 2008
Award to the besto oral presentation “Derrame pleural paraneumónico: epidemiología,
características clínicas y diagnóstico microbiológico” IV Congreso de la Sociedad Española de
Infectología Pediátrica. Marbella: 6- 8 marzo, 2008
Award to the best oral presentation. 56 Congress of Spanish Pediatric Association, year 2007.
“Utilidad de las técnicas de amplificación molecular en el diagnóstico de la enfermedad invasiva
meningocócica”
Award to the best oral presentation. 58 Congress of Spanish Pediatric Association, year 2009.
Enfermedad neumocócica invasiva pediátrica: serotipado y sensibilidad antibiótica en relación con la
vacuna antineumocócica conjugada heptavalente.
Award to the best poster presentation, year 2006 . Hospital Sant Joan de Déu. C.Muñoz-Almagro.
Increase of invasive infections caused by vaccine-related and non-vaccine serotypes of streptococcus
pneumoniae hmong children in Barcelona, Spain.
Award of Catalonian Society of Infectious Diseases and Clinical Microbiology, year 2006 “Detección
de la enfermedad invasiva severa pediátrica por Streptococcus pneumoniae mediante Real-Time PCR.
Criterios de gravedad y relación con la carga bacteriana en los pacientes ingresados en la unidad de
cuidados intensivos pediátricos”
Young Investigator Award of the Interdepartmental Research and Technological Innovation
Commission (CIRIT), 1991