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Dissections External Anatomy: Identify nares, eyes, ears, whiskers, and genitalia. Determine the sex of the mouse. What type of tissue is visible from external view? Let’s cut something Lay mouse on dorsal side (back); ventral side (belly) up. Use scalpel to make a small incision above the genitalia. Use scissors and pull away from mouse cutting towards the head up to the mouth. Remove arms and legs. Remove the skin and examine the skeletal muscle tissue. Remove the skeletal muscle and view the connective tissue that makes up the bone. Observe the ball-andsocket joint of the hip and the hinge joint of the knee. “Gimme an “I” Use scissors to make an incision from arm to arm and then from leg to leg. (Should look like a capital I) Examine layers of epithelial tissue (skin) and skeletal muscle tissue. Identify the liver and diaphragm (skeletal muscle tissue). Goodbye liver, hello digestive system Remove the liver identify the stomach, spleen, esophagus (pointer), small intestine, and large intestine. (Identify smooth muscle tissue and epithelial tissue) Cut the esophagus above the stomach and above the rectum and remove the digestive tract. stomach small intestine rectum In the female, identify the uterus (smooth muscle tissue) and fallopian tubes. Stretch out the intestine and measure in cm. Identify the kidneys and ureters. Locate adipose tissue on the ureters. Remove the kidneys. Cut the kidney in half and examine. Thoracotomy Time Open thoracic cavity by using scissors to cut midline through sternum, then clear ribs without cutting heart/lungs. Examine the heart (cardiac muscle tissue), lungs (epithelial tissue), trachea (connective tissue), and aorta (smooth muscle and connective tissue). You’re a, heart breaker… Remove the heart and identify the atria and ventricles. Left Atrium ventricle Flip mouse over to view dorsal side (back). Use scissors to remove skin from back. Locate spinal column in center of back. Remove mouse head and locate spinal cord (nervous tissue) in spinal column. Bonus points Remove the eye. Follow the spinal cord, use scissors to remove brain and identify nervous tissue. Use scissors to cut tail and identify nervous tissue in spinal cord. Clean-up Put mouse remains in trash bucket. Wash all dissecting tools and place on paper towel to dry. Wash out dissecting pan. Wipe down counters. Wash hands